Mark J Solomon PhD
Professor of Molecular Biophysics and Biochemistry
Cell cycle; Ubiquitin-mediated proteolysis; Anaphase-promoting Complex
My lab seeks to understand the eukaryotic cell cycle at a biochemical level. To get there, we use a variety of approaches ranging from genetics and “pseudo genetics” in yeast to biochemistry and enzyme assays using cell extracts and purified proteins from yeast and mammalian cells. We focus on two broad areas: Post-translational modification and activation of the cyclin-dependent kinases (CDKs) that control the cell cycle, and ubiquitin-dependent proteolysis of cyclins and other proteins by the Anaphase-Promoting Complex and how this proteolysis is controlled by cellular checkpoints. Progressing from genetic identification of components through their study in vitro we hope to achieve a more complete understanding of these macroscopic cellular behaviors.
- Ostapenko, D., J.L. Burton, and M.J. Solomon. (2012). Identification of Anaphase Promoting Complex Substrates in S. cerevisiae. PLoS ONE 7(9): e45895. doi: 10.1371/journal.pone.0045895.
- Wang, R. and M.J. Solomon. (2012). Identification of She3 as an SCFGrr1 substrate in budding yeast. PLoS ONE 7(10): e48020. doi: 10.1371/journal.pone.0048020.
- Burton J.L., Y. Xiong, and M.J. Solomon. (2011). Mechanisms of Pseudosubstrate Inhibition of the Anaphase Promoting Complex by Acm1. EMBO J., 30: 1818-1829.
- Ostapenko, D. and M.J. Solomon. (2011). Anaphase Promoting Complex-dependent Degradation of Transcriptional Repressors Nrm1 and Yhp1 in S. cerevisiae. Mol. Biol. Cell, 22: 2175-2184.
- Solomon, M.J. and J.L Burton. (2008). Securin’ M-phase Entry. Nat. Cell Biol., 10: 381-383.
- Ostapenko, D., J.L. Burton, R. Wang, and M.J. Solomon. (2008). Pseudosubstrate Inhibition of the Anaphase-Promoting Complex by Acm1: Regulation by Proteolysis and Cdc28 Phosphorylation. Mol. Cell. Biol., 28: 4653-4664.
- Cheng, A. and M.J. Solomon. (2008). Speedy/Ringo C Regulates S and G2 Phase Progression in Human Cells. Cell Cycle, 7: 3037-3047.
- Burton, J.L. and M.J. Solomon. (2007). Mad3p, a Pseudosubstrate Inhibitor of APC-Cdc20 in the Spindle Assembly Checkpoint. Genes & Dev., 21: 655-667.
- Burton, J.L., V. Tsakraklides, and M.J. Solomon. (2005). Assembly of an APC-Cdh1-Substrate Complex is Stimulated by Engagement of a Destruction Box. Mol. Cell, 18: 533-541.
- Cheng, A., S. Gerry, P. Kaldis, and M.J. Solomon. (2005). Biochemical Characterization of Cdk2-Speedy/Ringo. BMC Biochem., 6: 19 (1-17).