The Yale Genome Editing Center (YGEC) creates (and through cryopreservation, maintains) genetically engineered models for Yale investigator research. The YGEC has employed nuclease-mediated genome editing (CRISPR/Cas) technology to create precise genome changes, and continues to provide transgenic and knockout services as well to ensure the ability to create the best mouse model for your experiment. It offers mouse sperm and embryo cryopreservation as economical alternatives to maintaining genetically unique colonies of mice. The YGEC has brought together individuals with many years of experience in mouse embryo development, microinjection, ES cell derivation and culture, and cryopreservation. This allows users to enjoy a high success rate for their research projects and obviates the duplication of equipment and training that would occur without such a resource.
Specific services of the YGEC Resource include CRISPR/Cas microinjection of mouse zygotes to create genome-edited strains; pronuclear microinjection of DNA constructs to create transgenic strains; “traditional” embryonic stem (ES) cell based gene targeting; cryopreservation of 2.5d mouse embryos and of sperm.
Investigators can use YGEC, especially with the precision of genome editing, to create direct analogs of mutations involved in human disease, thus providing models that can be studied in a way that human subjects cannot. The ability to create such induced mutants allows investigators to effectively, and more rapidly than ever, use mouse genetics to dissect and analyze complicated developmental pathways, biochemical processes, and biomolecular interactions in a mammalian system.
Timothy Nottoli, PhD, Co-Director