2022
Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells
Kshitiz, Afzal J, Suhail Y, Chang H, Hubbi M, Hamidzadeh A, Goyal R, Liu Y, Sun P, Nicoli S, Dang C, Levchenko A. Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells. Cell Systems 2022, 13: 1048-1064.e7. PMID: 36462504, PMCID: PMC10012408, DOI: 10.1016/j.cels.2022.11.003.Peer-Reviewed Original ResearchConceptsHIF-1α activityActivation of genesChaperone-mediated autophagyCancer cellsCell divisionIndividual cancer cellsRegulated processPatient-derived cancer cellsSubset of cellsMolecular mechanismsFluorescent reportersSingle-cell responsesCancer cell linesCell linesGenesHypoxic tumor cellsHIF-1αHypoxic conditionsCellsBroad suppressionAggressive growthTumor cellsHypoxic cellsOscillatory activityExtracellular lactate
2002
The IκB-NF-κB Signaling Module: Temporal Control and Selective Gene Activation
Hoffmann A, Levchenko A, Scott M, Baltimore D. The IκB-NF-κB Signaling Module: Temporal Control and Selective Gene Activation. Science 2002, 298: 1241-1245. PMID: 12424381, DOI: 10.1126/science.1071914.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell NucleusChemokine CCL5Chemokine CXCL10Chemokines, CXCComputer SimulationCytoplasmDNA-Binding ProteinsElectrophoretic Mobility Shift AssayFeedback, PhysiologicalGene Expression RegulationHumansI-kappa B ProteinsMiceMice, KnockoutModels, BiologicalNF-kappa BNF-KappaB Inhibitor alphaProto-Oncogene ProteinsSignal TransductionTranscriptional ActivationTumor Cells, CulturedTumor Necrosis Factor-alphaConceptsTranscriptional activator NF-kappaBSelective gene activationKnockout cell linesTemporal controlNF-kappaB inhibitor proteinNF-kappaB responseSignaling modulesCoordinated degradationGene activationMammalian cellsNuclear localizationInhibitor proteinGene expressionIkappaB proteinsSignal-processing characteristicsEpsilon functionNF-kappaB activationCell linesNF-kappaB
2000
Evaluation of 11C-colchicine for PET imaging of multiple drug resistance.
Levchenko A, Mehta B, Lee J, Humm J, Augensen F, Squire O, Kothari P, Finn R, Leonard E, Larson S. Evaluation of 11C-colchicine for PET imaging of multiple drug resistance. Journal Of Nuclear Medicine 2000, 41: 493-501. PMID: 10716325.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 1Carbon RadioisotopesColchicineDrug Resistance, MultipleFlow CytometryHumansMiceMice, Inbred BALB CModels, TheoreticalNeuroblastomaRatsRats, NudeTissue DistributionTomography, Emission-ComputedTransplantation, HeterologousTumor Cells, CulturedConceptsResistant tumorsPET imagingMultiple drug resistance phenotypeDrug-resistant variantsQuality of lifeCell linesMultiple drug resistanceResistant cell linesC cell lineP-gp actionDrug resistance phenotypeEarly diagnosisNude ratsFDG scansTumorsP-glycoproteinDrug resistanceBiodistribution experimentsCytotoxic agentsChemotherapeutic interventionResistant strainsCancer cellsIntracellular accumulationVivo experimentsScans