1998
A single amino acid substitution in a WW‐like domain of diverse members of the PDGF receptor subfamily of tyrosine kinases causes constitutive receptor activation
Irusta P, DiMaio D. A single amino acid substitution in a WW‐like domain of diverse members of the PDGF receptor subfamily of tyrosine kinases causes constitutive receptor activation. The EMBO Journal 1998, 17: 6912-6923. PMID: 9843497, PMCID: PMC1171039, DOI: 10.1093/emboj/17.23.6912.Peer-Reviewed Original ResearchMeSH KeywordsAlanineAmino Acid SequenceAmino Acid SubstitutionAnimalsBinding SitesCell Line, TransformedCloning, MolecularDimerizationEnzyme ActivationHumansInterleukin-3LigandsMiceMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphorylationPolymerase Chain ReactionReceptor Protein-Tyrosine KinasesReceptor, Platelet-Derived Growth Factor betaReceptors, Platelet-Derived Growth FactorSequence Homology, Amino AcidStructure-Activity RelationshipTyrosineValineConceptsTyrosine kinase activityKinase activityTyrosine kinasePlatelet-derived growth factor beta receptorCytoplasmic juxtamembrane domainPDGF receptorSignal transduction proteinsWW-like domainTransmembrane receptor tyrosine kinaseProtein-protein interactionsBa/F3 cellsGST fusion proteinSingle amino acid substitutionConstitutive receptor activationGrowth factor beta receptorAbsence of ligandReceptor tyrosine kinasesAmino acid substitutionsSequence PPXYTransduction proteinsWW domainsCellular functionsJuxtamembrane regionTyrosine phosphorylationAlanine substitutions
1995
The HPV16 E5 Protein: Expression, Detection, and Stable Complex Formation with Transmembrane Proteins in COS Cells
Hwang E, Nottoli T, Dimaio D. The HPV16 E5 Protein: Expression, Detection, and Stable Complex Formation with Transmembrane Proteins in COS Cells. Virology 1995, 211: 227-233. PMID: 7645215, DOI: 10.1006/viro.1995.1395.Peer-Reviewed Original ResearchConceptsE5 proteinHPV16 E5 proteinStable complex formationTransmembrane proteinGrowth factor receptorE5 genePlatelet-derived growth factor beta receptorViral proteinsVesicular stomatitis virus glycoproteinComplex formationFactor receptorCOS monkey cellsGrowth factor beta receptorStable growth transformationCultured cell systemsEpidermal growth factor receptorFactor 1 receptorTransforming proteinCoimmunoprecipitation analysisCOS cellsExpression vectorMonkey cellsBiochemical propertiesProteinE5 expression
1994
Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes
Kegler-Ebo D, Docktor C, DiMaio D. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes. Nucleic Acids Research 1994, 22: 1593-1599. PMID: 8202358, PMCID: PMC308034, DOI: 10.1093/nar/22.9.1593.Peer-Reviewed Original Research
1993
Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells.
Andrews E, DiMaio D. Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells. Journal Of Virology 1993, 67: 7705-10. PMID: 7901430, PMCID: PMC238246, DOI: 10.1128/jvi.67.12.7705-7710.1993.Peer-Reviewed Original ResearchAnimalsAntisense Elements (Genetics)Base SequenceBovine papillomavirus 1Cell Transformation, ViralCells, CulturedCloning, MolecularConsensus SequenceDNA, ComplementaryMiceMolecular Sequence DataMutagenesisPoly APolymerase Chain ReactionRegulatory Sequences, Nucleic AcidRNA Processing, Post-TranscriptionalRNA, MessengerSequence Analysis, DNA
1992
A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase.
Goldstein D, Kulke R, Dimaio D, Schlegel R. A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase. Journal Of Virology 1992, 66: 405-13. PMID: 1370089, PMCID: PMC238300, DOI: 10.1128/jvi.66.1.405-413.1992.Peer-Reviewed Original ResearchConceptsK proteinGrowth factor receptorE5 oncoproteinGlutamine residuesRandom hydrophobic sequencesSpecific amino acid residuesMembrane-associated domainMajor transforming proteinK protein componentCarboxyl-terminal domainEndoplasmic reticulum membraneFactor receptorBovine papillomavirus type 1Colony-stimulating factor 1 receptorTransformation-defective mutantsAmino acid residuesPotential binding sitesPlatelet-derived growth factor receptorAmino acid substitutionsPapillomavirus type 1Hydrophilic amino acidsE5 dimerE5 mutantsFactor 1 receptorProtein complexes
1991
Biological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor.
Kulke R, DiMaio D. Biological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor. Journal Of Virology 1991, 65: 4943-9. PMID: 1651413, PMCID: PMC248956, DOI: 10.1128/jvi.65.9.4943-4949.1991.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, NorthernCell Transformation, ViralCloning, MolecularDeerDNAGene ExpressionGenes, ViralIn Vitro TechniquesMiceMolecular Sequence DataOncogene Proteins, ViralPapillomaviridaePlatelet-Derived Growth FactorReceptors, Cell SurfaceReceptors, Platelet-Derived Growth FactorRNA, ViralViral Structural ProteinsConceptsMouse C127 cellsE5 proteinC127 cellsE5 genePlatelet-derived growth factor beta receptorPDGF receptorBovine papillomavirus type 1 E5 proteinConstitutive tyrosine phosphorylationDNA synthesisGrowth factor beta receptorBovine papillomavirus type 1Platelet-derived growth factor receptorTransformation of fibroblastsPapillomavirus type 1Sequence similarityGrowth factor receptorTyrosine phosphorylationBiological activityShort regionFoci formationProteinFactor receptorReceptor formsB chainGrowth transformation
1990
[45] Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil
Horwitz B, Dimaio D. [45] Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil. Methods In Enzymology 1990, 185: 599-611. PMID: 2166223, DOI: 10.1016/0076-6879(90)85047-r.Peer-Reviewed Original Research
1986
Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity.
DiMaio D, Guralski D, Schiller J. Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1797-1801. PMID: 3006073, PMCID: PMC323171, DOI: 10.1073/pnas.83.6.1797.Peer-Reviewed Original ResearchConceptsMouse C127 cellsC127 cellsTransformation defectGenetic mapping experimentsCorrect reading frameStrong genetic evidenceBovine papillomavirus type 1Series of mutationsViral DNAPapillomavirus type 1Putative genesGenetic evidenceHydrophobic proteinsReading frameFrameshift mutantsPolypeptide domainsBovine papillomavirus DNAOncogenic transformationDifferent mutantsORFSecond mutationSequence changesMutantsMapping experimentsFrameshift mutation
1981
Directed Mutagenesis
Shortle D, DiMaio D, Nathans D. Directed Mutagenesis. Annual Review Of Genetics 1981, 15: 265-294. PMID: 6279018, DOI: 10.1146/annurev.ge.15.120181.001405.Peer-Reviewed Original Research