Darin Dolezal, MD, PhD
InstructorCards
About
Research
Overview
The goal of one ongoing research project, which is supported by an intradepartmental Chair’s Challenge grant, is to detect driver and resistance mutations in pleural fluid-derived circulating tumor DNA from patients with lung adenocarcinoma. The aim of this project is to use an error-suppressed multiplex deep sequencing platform, originally designed to detect low abundance tumor-specific mutations in plasma, to detect mutations with high sensitivity in the supernatants of malignant pleural effusions.
Publications
2025
Comprehensive Molecular Profiling of Metastatic Pancreatic Adenocarcinomas
Antony V, Sun T, Dolezal D, Cai G. Comprehensive Molecular Profiling of Metastatic Pancreatic Adenocarcinomas. Cancers 2025, 17: 335. DOI: 10.3390/cancers17030335.Peer-Reviewed Original ResearchMetastatic pancreatic ductal adenocarcinomaPancreatic ductal adenocarcinomaPrimary pancreatic ductal adenocarcinomaMolecular profilingGene mutationsGene copy number alterationsAdvanced-stage pancreatic cancerInsufficient tumor cellsOncomine Comprehensive AssayRate of TP53Copy number alterationsMetastatic diseasePDAC casesPIK3CA mutationsPancreatic cancerPoor prognosisTreatment optionsDuctal adenocarcinomaTumor cellsTumor progressionMolecular alterationsStudy cohortMolecular testingComprehensive assayTherapeutic target
2024
BSLM-10 MOLECULAR AND HISTOLOGICAL CHARACTERIZATION OF NSCLC PROGRESSION TO LEPTOMENINGEAL METASTASIS WITH COMORBID INTRAPARENCHYMAL DISEASE
Kandigian S, Chande S, Dolezal D, Tang T, Wang D, Arnal-Estapé A, Cheok S, McGuone D, Liu Y, Goldberg S, Blondin N, Chiang V, Nguyen D. BSLM-10 MOLECULAR AND HISTOLOGICAL CHARACTERIZATION OF NSCLC PROGRESSION TO LEPTOMENINGEAL METASTASIS WITH COMORBID INTRAPARENCHYMAL DISEASE. Neuro-Oncology Advances 2024, 6: i7-i7. PMCID: PMC11296776, DOI: 10.1093/noajnl/vdae090.020.Peer-Reviewed Original ResearchNon-small cell lung cancerLeptomeningeal diseaseCentral nervous systemLeptomeningeal metastasesParenchymal metastasesCerebrospinal fluidTumor cellsTyrosine kinase inhibitor treatmentCell lung cancerKinase inhibitor treatmentCerebrospinal fluid of patientsCell linesCerebral lateral ventriclesIntra-arterial injectionTGF-b signalingIn vivo passageIntraparenchymal diseaseMechanisms of progressionTumor microenvironmentMultiplex immunofluorescenceAggressive treatmentLeptomeningeal infiltrationPerivascular invasionIntraparenchymal metastasesMurine modelThe World Health Organization Reporting System for Lung Cytopathology–A Review of the First Edition
Dolezal D, Kholová I, Cai G. The World Health Organization Reporting System for Lung Cytopathology–A Review of the First Edition. Journal Of Clinical And Translational Pathology 2024, 4: 18-35. PMID: 38736711, PMCID: PMC11086742, DOI: 10.14218/jctp.2023.00068.Peer-Reviewed Original ResearchClinical management recommendationsRisk of malignancyCytomorphologic criteriaLung cytopathologyImprove patient careAncillary testsEstimated risk of malignancyReporting SystemDiagnostic categoriesPatient carePotential diagnostic pitfallsGroup of lesionsPractice settingsManagement recommendationsPractice recommendationsDiagnostic pitfallsEstimated riskCytopathology specimensCytopathology practiceLungCytopathologyLesionsMalignancyCytopathologistsSpecimen types
2023
Breast Carcinoma With Tubulopapillary Features Has a Distinct Immunophenotypic and Molecular Signature: A Report of Two Tumors and Literature Review
Carrasco-Tenezaca F, Moreira-Dinzey J, Manrai P, Bearse M, Burela S, Podany P, Singh K, Pareja F, Zheng J, Muscato N, Liang Y, Zhan H, Krishnamurti U, Dolezal D, Wang J, Harigopal M. Breast Carcinoma With Tubulopapillary Features Has a Distinct Immunophenotypic and Molecular Signature: A Report of Two Tumors and Literature Review. International Journal Of Surgical Pathology 2023, 32: 1037-1045. PMID: 37908113, DOI: 10.1177/10668969231209780.Peer-Reviewed Original ResearchBreast carcinomaTubulopapillary featuresHuman epidermal growth factor 2 (HER2) expressionBreast cancer histologic subtypesNonmetastatic breast carcinomaTriple-negative profileHigh-grade nuclear featuresTriple-negative carcinomasHigh-grade featuresMetastatic breast carcinomaGrowth factor 2 expressionFactor 2 expressionReports of tumorsMicropapillary projectionsNeoadjuvant therapyHistologic subtypeWorse prognosisFirst tumorPoor prognosisAdditional tumorsInvasive carcinomaEstrogen receptorCarcinomaPathogenic variantsTumorsS2209 Pancreatic Insulinoma: A Hidden Challenge
Mathialagan K, Dolezal D, Nagar A. S2209 Pancreatic Insulinoma: A Hidden Challenge. The American Journal Of Gastroenterology 2023, 118: s1585-s1585. DOI: 10.14309/01.ajg.0000958476.17153.c1.Peer-Reviewed Original Research