1988
Resolution of Acanthamoeba castellanii chromosomes by pulsed field gel electrophoresis and construction of the initial linkage map
Rimm D, Pollard T, Hieter P. Resolution of Acanthamoeba castellanii chromosomes by pulsed field gel electrophoresis and construction of the initial linkage map. Chromosoma 1988, 97: 219-223. PMID: 3219918, DOI: 10.1007/bf00292964.Peer-Reviewed Original ResearchConceptsChromosome-sized DNA moleculesMyosin II geneGel electrophoresisDNA moleculesField gel electrophoresisNon-allelic genesProtozoan Acanthamoeba castellaniiDifferent chromosomal bandsLower eukaryotesLinkage mapSmall chromosomesLarge chromosomesLinkage groupsElectrophoretic karyotypeChromosomal bandsChromosome bandsMyosin IBChromosomesAcanthamoeba castellaniiGenesBiochemical researchElectrophoresisEukaryotesDictyosteliumFungi
1986
Characterization of alpha‐actinin from Acanthamoeba
Pollard T, Tseng P, Rimm D, Bichell D, Williams R, Sinard J, Sato M. Characterization of alpha‐actinin from Acanthamoeba. Cytoskeleton 1986, 6: 649-661. PMID: 2948678, DOI: 10.1002/cm.970060613.Peer-Reviewed Original ResearchConceptsCross-linking proteinsActin filamentsActin filament cross-linking proteinCross-link actin filamentsCross-links actin filamentsAmino acid compositionAmoeba proteinApparent molecular weightActin polymerizationGlobular domainElectrophoretic variantsNative proteinCell extractsActin monomersPure proteinProteinGel electrophoresisAmoebaeCytoplasmic matrixStokes radiusMolecular weightPolypeptideAcid compositionIndirect fluorescent antibody stainingAntibody staining