2021
Selective multiplexed enrichment for the detection and quantitation of low-fraction DNA variants via low-depth sequencing
Song P, Chen SX, Yan YH, Pinto A, Cheng LY, Dai P, Patel AA, Zhang DY. Selective multiplexed enrichment for the detection and quantitation of low-fraction DNA variants via low-depth sequencing. Nature Biomedical Engineering 2021, 5: 690-701. PMID: 33941896, PMCID: PMC9631981, DOI: 10.1038/s41551-021-00713-0.Peer-Reviewed Original Research
2002
The splicing of U12‐type introns can be a rate‐limiting step in gene expression
Patel AA, McCarthy M, Steitz JA. The splicing of U12‐type introns can be a rate‐limiting step in gene expression. The EMBO Journal 2002, 21: 3804-3815. PMID: 12110592, PMCID: PMC126102, DOI: 10.1093/emboj/cdf297.Peer-Reviewed Original ResearchConceptsU12-type intronsGene expressionDrosophila melanogaster S2 cellsProtein-coding genesU12-type spliceosomePost-transcriptional regulationHuman tissue culture cellsU2-type intronsMetazoan genomesTissue culture cellsS2 cellsU12-typeIntron removalIdentical mRNAIntronsFluorescent proteinQuantitative RT-PCR assayMinigene constructsCulture cellsRate-limiting stepSpliceosomeMRNAMinor classExpressionRT-PCR assays