2023
The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection
McCollum C, Didychuk A, Liu D, Murray-Nerger L, Cristea I, Glaunsinger B. The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection. PLOS Pathogens 2023, 19: e1011163. PMID: 37068108, PMCID: PMC10138851, DOI: 10.1371/journal.ppat.1011163.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusGene expressionViral transcriptionKSHV gene expressionCellular RNA polymerasesHerpesvirus gene expressionTranscriptional activator proteinViral DNA packaging motorsPotential gene expressionTranscriptional activation complexLate gene expressionDNA packaging motorRNA polymeraseProximity labelingHost transcriptionSiRNA screenSarcoma-associated herpesvirusLate genesTranscription complexLate proteinsCatalytic subunitActivator proteinPackaging motorORF29Viral packaging
2020
Conserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34
Didychuk A, Castañeda A, Kushnir L, Huang C, Glaunsinger B. Conserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34. Journal Of Virology 2020, 94: 10.1128/jvi.01299-19. PMID: 31578296, PMCID: PMC6955276, DOI: 10.1128/jvi.01299-19.Peer-Reviewed Original ResearchConceptsViral preinitiation complexKaposi's sarcoma-associated herpesvirusC-terminal domainCysteine-rich motifHost transcription machineryPreinitiation complexGene promoterTranscription machinerySarcoma-associated herpesvirusLate genesGene transcriptionViral late gene expressionZinc finger motifsLate gene transcriptionSequence-specific bindingTranscriptional regulatory activityLate gene expressionLate gene promotersInfectious virionsProduction of capsid proteinsRelease of infectious virionsViral replication cycleFinger motifPromoter occupancySequence-specific