Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly
Didychuk A, Montemayor E, Brow D, Butcher S. Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly. Nucleic Acids Research 2015, 44: 1398-1410. PMID: 26673715, PMCID: PMC4756825, DOI: 10.1093/nar/gkv1374.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding, CompetitiveKineticsModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationProtein BindingProtein Structure, TertiaryRibonucleoprotein, U4-U6 Small NuclearRibonucleoproteins, Small NuclearRNA, FungalRNA, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsU6 RNADi-snRNPRemodeling of RNA structureAnnealing in vitroTri-snRNP complexLarge-scale remodelingStable ternary complexRNA bindingRNA structureRibonucleoprotein complexU6 snRNPU6 snRNABase pairsPrp24RNATernary complexU4/U6Electropositive characterRate enhancementAssemblyStructural requirementsSnRNASnRNPLsm2Annealing rateStructural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA
Cornilescu G, Didychuk A, Rodgers M, Michael L, Burke J, Montemayor E, Hoskins A, Butcher S. Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal Of Molecular Biology 2015, 428: 777-789. PMID: 26655855, PMCID: PMC4790120, DOI: 10.1016/j.jmb.2015.11.026.Peer-Reviewed Original ResearchConceptsU4/U6 di-snRNAScattering dataTri-snRNPAnalysis of RNA structureCryo-electron microscopy structureResonance energy transfer dataX-ray scattering dataMagnetic susceptibility anisotropyRNA structureAssembled spliceosomeRibonucleoprotein complexTertiary interactionsU4/U6Energy transfer dataAlignment mediaSusceptibility anisotropyCryo-electronAlignment tensorStructural investigationsRNAX-rayNucleic acidsStructural analysisWAXS dataY-shaped structure
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