2007
Loss of T cell responses following long-term cryopreservation
Owen RE, Sinclair E, Emu B, Heitman JW, Hirschkorn DF, Epling CL, Tan QX, Custer B, Harris JM, Jacobson MA, McCune JM, Martin JN, Hecht FM, Deeks SG, Norris PJ. Loss of T cell responses following long-term cryopreservation. Journal Of Immunological Methods 2007, 326: 93-115. PMID: 17707394, PMCID: PMC2065759, DOI: 10.1016/j.jim.2007.07.012.Peer-Reviewed Original ResearchConceptsT cell IFN-gamma responsesT cell responsesHuman immunodeficiency virusIFN-gamma responsesPeripheral blood mononuclear cellsCell responsesLong-term cryopreservationPeptide poolsHIV-negative individualsBlood mononuclear cellsLoss of responseT cell apoptosisPhenotypic marker expressionDecrement of responseOvernight restingEffector cellsImmunodeficiency virusExogenous antigensMononuclear cellsShort-term cryopreservationHIV peptidesFuture vaccinesImpact of cryopreservationPathogenesis studiesMarker expression
2006
IL-2 production correlates with effector cell differentiation in HIV-specific CD8+ T cells
Nomura LE, Emu B, Hoh R, Haaland P, Deeks SG, Martin JN, McCune JM, Nixon DF, Maecker HT. IL-2 production correlates with effector cell differentiation in HIV-specific CD8+ T cells. AIDS Research And Therapy 2006, 3: 18. PMID: 16859558, PMCID: PMC1562434, DOI: 10.1186/1742-6405-3-18.Peer-Reviewed Original ResearchHIV-specific CD8HIV-positive subjectsHIV-specific T cellsCMV-specific T cellsEffector cell differentiationT cellsIL-2Effector cellsIL-2 production correlatesMemory/effector phenotypeTerminal effector cellsAntigen-specific CD8Cytokine flow cytometryHIV-negative subjectsIL-2 productionCD27-CD28HIV-negativeCytokine profileProgressive diseaseEffector phenotypeHIV-specificEffector differentiationCD8Cell differentiationCell frequency