2019
How Complementary Targets Expose the microRNA 3′ End for Tailing and Trimming during Target-Directed microRNA Degradation
Pawlica P, Sheu-Gruttadauria J, MacRae IJ, Steitz JA. How Complementary Targets Expose the microRNA 3′ End for Tailing and Trimming during Target-Directed microRNA Degradation. Cold Spring Harbor Symposia On Quantitative Biology 2019, 84: 039321. PMID: 32019864, PMCID: PMC9161719, DOI: 10.1101/sqb.2019.84.039321.Peer-Reviewed Original ResearchTarget-directed miRNA degradationMiRNA 3' endCellular enzymesMiRNA seed sequencesMiRNA complementarityMiRNA decayArgonaute proteinsMicroRNA degradationMiRNA degradationMiRNA 5Posttranscriptional regulationMiRNA targetsCertain transcriptsSeed sequenceExtensive complementarityMolecular mechanismsMessenger RNATranscriptsEnzymeRegulationEnzymatic attackRecent advancesTargetMicroRNAsRNA
1996
A mammalian gene with introns instead of exons generating stable RNA products
Tycowski K, Shu M, Steitz J. A mammalian gene with introns instead of exons generating stable RNA products. Nature 1996, 379: 464-466. PMID: 8559254, DOI: 10.1038/379464a0.Peer-Reviewed Original ResearchConceptsProtein-coding genesRibosomal subunit assemblyRibosomal RNA transcriptionSmall nucleolar RNAsMammalian genesSnoRNA genesEukaryotic cellsProtein codingMature rRNANucleolar RNAsHost genesSubunit assemblyDifferent intronsRNA transcriptionRNA productsExtensive complementarityIntronsGenesSnoRNAsExonsRNARRNATranscriptionPolysomesMaturation1
1980
Are snRNPs involved in splicing?
Lerner M, Boyle J, Mount S, Wolin S, Steitz J. Are snRNPs involved in splicing? Nature 1980, 283: 220-224. PMID: 7350545, DOI: 10.1038/283220a0.Peer-Reviewed Original ResearchConceptsSmall nuclear RNA speciesProminent nuclear proteinsRNA-protein complexesSmall RNA moleculesSmall nuclear ribonucleoproteinNuclear RNA speciesSnRNA speciesSnRNAs U1RNA speciesU6 RNASnRNA moleculesNuclear proteinsNuclear ribonucleoproteinNucleotide sequenceU1 RNARNA moleculesSnRNPsExtensive complementaritySplice junctionsNuclear locationLines of evidenceAnti-Sm seraCell nucleiHnRNA moleculesMouse Ehrlich
1978
Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells
Hagenbüchle O, Santer M, Steitz J, Mans R. Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells. Cell 1978, 13: 551-563. PMID: 77738, DOI: 10.1016/0092-8674(78)90328-8.Peer-Reviewed Original ResearchConceptsE. coli 16S rRNAE. coli ribosomesPurine-rich regionDNA sequencing methodsRRNA moleculesEucaryotic speciesStrong homologyCDNA transcriptsCDNA copyPolynucleotidyl transferasesRNA moleculesRRNA sequencesEucaryotic cellsEucaryotic mRNAsExtensive complementarityColi ribosomesRRNAPrimary structureEnzyme ATPSequencing methodsOligonucleotide primersAMV reverse transcriptaseTerminusReverse transcriptaseSequence