2024
IL-1 receptor 1 signaling shapes the development of viral antigen-specific CD4+ T cell responses following COVID-19 mRNA vaccination
Park H, Shin M, Shin J, Kim H, Kang B, Par-Young J, Unlu S, Afinogenova Y, Catanzaro J, Young J, Kim M, Lee S, Jeon S, You S, Racke M, Bucala R, Kang I. IL-1 receptor 1 signaling shapes the development of viral antigen-specific CD4+ T cell responses following COVID-19 mRNA vaccination. EBioMedicine 2024, 103: 105114. PMID: 38640835, PMCID: PMC11041015, DOI: 10.1016/j.ebiom.2024.105114.Peer-Reviewed Original ResearchConceptsCD4<sup>+</sup> T cellsCOVID-19 mRNA vaccinesAntigen-specific CD4<sup>+</sup> T cell responsesT cell responsesPrimary antibody deficiencyCD4<sup>+</sup> T cell responsesT cellsIL-1R1MRNA vaccinesIL-1IgG antibodiesAntigen-specific CD4<sup>+</sup> T cellsCD4+ T cell responsesLevels of IL-1R1Human CD4<sup>+</sup> T cellsIL-1 receptor 1Healthy individualsDose of COVID-19 mRNA vaccineAntigen-specific CD4IL-1R1 expressionT cell immunityRepetitive antigenic stimulationCytokines interleukin (IL)-1Immune response to virusesExpression of IL-1R1
2023
Aging gene signature of memory CD8+ T cells is associated with neurocognitive functioning in Alzheimer’s disease
Young J, Park H, Kim M, Par-Young J, Bartlett H, Kim H, Unlu S, Osmani L, Shin M, Bucala R, van Dyck C, Allore H, Mecca A, You S, Kang I. Aging gene signature of memory CD8+ T cells is associated with neurocognitive functioning in Alzheimer’s disease. Immunity & Ageing 2023, 20: 71. PMID: 38042785, PMCID: PMC10693128, DOI: 10.1186/s12979-023-00396-y.Peer-Reviewed Original ResearchPeripheral bloodT cellsAlzheimer's diseaseEM CD8Memory CD8Gene signatureAge-related immune changesIL-7 receptor alphaEffector memory CD8Strong risk factorT cell expansionAD genesAge-associated expansionImmune changesRisk factorsCD8Dementia patientsIL-7RNeuropsychological testingReceptor alphaNeurocognitive functionRT-qPCR resultsDisease severityPatientsNormal personsModulating IL-1β and its receptors shapes spike-specific CD4 +T cell responses to mRNA vaccination
Park H, Shin M, Shin J, Kim H, Kang B, Par-Young J, Unlu S, Afinogenova Y, Catanzaro J, Young J, Kim M, Lee S, You S, Racke M, Bucala R, Kang I. Modulating IL-1β and its receptors shapes spike-specific CD4 +T cell responses to mRNA vaccination. The Journal Of Immunology 2023, 210: 159.20-159.20. DOI: 10.4049/jimmunol.210.supp.159.20.Peer-Reviewed Original ResearchCOVID-19 mRNA vaccinesT cellsIL-1βIL-1R2MRNA vaccinesIL-1 receptor 1IL-1R1 expressionIL-1R2 expressionSpike-specific CD4Vaccine-induced CD4T cell immunityT cell responsesSpecific antibody productionMRNA vaccinationCell immunityDurable immunityIL-1R1IL-1BCD4Healthy individualsQuest DiagnosticsAntibody productionCell responsesReceptor 1Receptor system
2022
Defining Clinical and Immunological Predictors of Poor Immune Responses to COVID-19 mRNA Vaccines in Patients with Primary Antibody Deficiency
Shin JJ, Par-Young J, Unlu S, McNamara A, Park HJ, Shin MS, Gee RJ, Doyle H, Afinogenova Y, Zidan E, Kwah J, Russo A, Mamula M, Hsu FI, Catanzaro J, Racke M, Bucala R, Wilen C, Kang I. Defining Clinical and Immunological Predictors of Poor Immune Responses to COVID-19 mRNA Vaccines in Patients with Primary Antibody Deficiency. Journal Of Clinical Immunology 2022, 42: 1137-1150. PMID: 35713752, PMCID: PMC9203263, DOI: 10.1007/s10875-022-01296-4.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, ViralCD8-Positive T-LymphocytesCommon Variable ImmunodeficiencyCOVID-19COVID-19 VaccinesHumansImmunity, CellularImmunoglobulin AImmunoglobulin GMRNA VaccinesPrimary Immunodeficiency DiseasesRNA, MessengerSARS-CoV-2Spike Glycoprotein, CoronavirusVaccinationVaccinesVaccines, SyntheticConceptsCommon variable immune deficiencyT cellsImmune responseIgG responsesCVID patientsMRNA vaccinesB cellsCoronavirus disease 2019 (COVID-19) mRNA vaccinesCOVID-19 mRNA vaccinesBaseline immune profileHistory of autoimmunityPrimary antibody deficiencyT cell responsesCellular immune responsesPoor immune responseVariable immune deficiencyMemory B cellsSARS-CoV-2 spike proteinBaseline IgGCVID diagnosisEM CD8Immunological predictorsPAD cohortSpecific CD4Immune profile
2021
ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus
Yao J, Leng L, Fu W, Li J, Bronner C, Bucala R. ICBP90 Regulates MIF Expression, Glucocorticoid Sensitivity, and Apoptosis at the MIF Immune Susceptibility Locus. Arthritis & Rheumatology 2021, 73: 1931-1942. PMID: 33844457, DOI: 10.1002/art.41753.Peer-Reviewed Original ResearchMeSH KeywordsAllelesApoptosisCCAAT-Enhancer-Binding ProteinsCell LineFibroblastsGene Expression RegulationGenetic LociGenetic Predisposition to DiseaseGenotypeGlucocorticoidsHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsPromoter Regions, GeneticReceptors, GlucocorticoidUbiquitin-Protein LigasesConceptsMacrophage migration inhibitory factorAutoimmune disease severityMIF -794 CATTRheumatoid synovial fibroblastsMIF expressionGlucocorticoid receptorGlucocorticoid sensitivitySynovial fibroblastsT cellsDisease severityPeripheral blood T cellsBlood T cellsMigration inhibitory factorGlucocorticoid-treated cellsAP-1Glucocorticoid immunosuppressionMIF promoterMIF genotypeMIF polymorphismsFactor antibodyInflammatory responseT lymphocytesActivator protein-1Glucocorticoid responsivenessInhibitory factor
2019
MIF and MIF2 have distinct but synergistic roles in CIA pathogenesis
Doherty E, Tilstam P, Bucala R. MIF and MIF2 have distinct but synergistic roles in CIA pathogenesis. The Journal Of Immunology 2019, 202: 180.13-180.13. DOI: 10.4049/jimmunol.202.supp.180.13.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorRheumatoid arthritisT cellsCIA inductionCollagen-induced arthritis (CIA) mouse modelSevere erosive rheumatoid arthritisEffector memory T cellsCytokine macrophage migration inhibitory factorRelapsing autoimmune diseaseErosive rheumatoid arthritisProgressive joint destructionT cell infiltrationMemory T cellsAbstract Rheumatoid arthritisArthritis mouse modelSecondary lymphoid organsMigration inhibitory factorCIA pathogenesisHigh expression alleleJoint inflammationJoint destructionLymph nodesWT miceAutoimmune diseasesReceptor CD74
2017
Adoptive transfer of a novel MIF receptor (CD74+) expressing memory T cell subpopulation is sufficient to transfer inflammatory arthritis
Doherty E, Piecychna M, Leng L, Bucala R. Adoptive transfer of a novel MIF receptor (CD74+) expressing memory T cell subpopulation is sufficient to transfer inflammatory arthritis. The Journal Of Immunology 2017, 198: 156.3-156.3. DOI: 10.4049/jimmunol.198.supp.156.3.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorEffector memory T cellsMemory T cellsRheumatoid arthritisT cellsJoint inflammationAdoptive transferCollagen-induced arthritis (CIA) mouse modelSevere erosive rheumatoid arthritisCytokine macrophage migration inhibitory factorMemory T-cell subpopulationsAutoimmune joint inflammationImmune checkpoints CTLA4MIF receptor CD74Effector memory phenotypeErosive rheumatoid arthritisAbstract Rheumatoid arthritisArthritis mouse modelT cell subpopulationsMigration inhibitory factorT cell activationExpression of STAT4High expression alleleInflammatory arthritisMIF receptor
2001
Peripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites
Abe R, Donnelly S, Peng T, Bucala R, Metz C. Peripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites. The Journal Of Immunology 2001, 166: 7556-7562. PMID: 11390511, DOI: 10.4049/jimmunol.166.12.7556.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCell DifferentiationCell MovementCells, CulturedCollagenFemaleFibroblastsFibrosisGelsHumansInjections, IntravenousLipopolysaccharide ReceptorsMiceMice, Inbred BALB CReceptors, ChemokineStem Cell TransplantationStem CellsTransforming Growth Factor betaTransforming Growth Factor beta1Wound HealingConceptsCultured fibrocytesTissue injuryChemokine/chemokine receptor interactionsUnique cell surface phenotypeCutaneous tissue injurySecondary lymphoid chemokineAlpha-smooth muscle actinWound healingWound healing myofibroblastsMononuclear cell populationsCCR7 chemokine receptorChemokine receptor interactionsPotent immunostimulatory activitySmooth muscle actinCell surface phenotypeBlood-borne cellsDifferentiation pathwayFibrocyte traffickingLymphoid chemokinesFibrocyte differentiationChemokine receptorsT cellsSurface phenotypePotent stimulusMuscle actinRegulation of the CTL Response by Macrophage Migration Inhibitory Factor
Abe R, Peng T, Sailors J, Bucala R, Metz C. Regulation of the CTL Response by Macrophage Migration Inhibitory Factor. The Journal Of Immunology 2001, 166: 747-753. PMID: 11145646, DOI: 10.4049/jimmunol.166.2.747.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAdoptive TransferAnimalsAntibodies, MonoclonalCD8-Positive T-LymphocytesCell MovementCells, CulturedCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFemaleInjections, IntraperitonealLymphocytes, Tumor-InfiltratingMacrophage Migration-Inhibitory FactorsMiceMice, Inbred C57BLNeoplasm TransplantationT-Lymphocytes, CytotoxicThymomaTumor Cells, CulturedConceptsMacrophage migration inhibitory factorMigration inhibitory factorTumor-bearing miceCTL activityCTL responsesT lymphocytesAnti-tumor T lymphocytesInhibitory factorCultures of splenocytesApoptotic tumor cellsT cell survivalIL-2RPivotal cytokineT cellsHistological examinationImmune responseIFN-gammaSplenocyte culturesAg stimulationMouse modelTumor growthTumor tissueTumor cellsMiceElevated expression
2000
Peripheral blood fibrocytes: Mesenchymal precursor cells and the pathogenesis of fibrosis
Chesney J, Bucala R. Peripheral blood fibrocytes: Mesenchymal precursor cells and the pathogenesis of fibrosis. Current Rheumatology Reports 2000, 2: 501-505. PMID: 11123104, DOI: 10.1007/s11926-000-0027-5.Peer-Reviewed Original ResearchConceptsPeripheral blood fibrocytesBlood fibrocytesTissue injuryDistinct cell surface phenotypePersistent T-cell activationNaïve T cellsPathogenesis of fibrosisCell surface phenotypeT cell activationCognate immunityAutoimmune disordersConnective tissue cellsImmunohistochemical studyForeign antigensT cellsSurface phenotypeMesenchymal precursor cellsScar formationFibrotic tissueFibrocytesGrowth factorPrecursor cellsNovel populationMatrix depositionInjury
1999
Interaction of Borrelia burgdorferi with Peripheral Blood Fibrocytes, Antigen-Presenting Cells with the Potential for Connective Tissue Targeting
Grab D, Lanners H, Martin L, Chesney J, Cai C, Adkisson H, Bucala R. Interaction of Borrelia burgdorferi with Peripheral Blood Fibrocytes, Antigen-Presenting Cells with the Potential for Connective Tissue Targeting. Molecular Medicine 1999, 5: 46-54. PMID: 10072447, PMCID: PMC2230375, DOI: 10.1007/bf03402138.Peer-Reviewed Original ResearchConceptsPeripheral blood fibrocytesBlood fibrocytesDifferent cell typesB. burgdorferiB. burgdorferi bindsInteraction of BorreliaMolecular mechanismsOspB proteinsAntigen presenting cellsCell typesFibroblast-like cellsCellular interactionsCell membraneJoint connective tissuesCollagen type ILyme arthritisPresent antigensInflammatory processT cellsFunctional capacityCellular collagenImmune systemFlow cytometryFibrocytesConnective tissue
1998
Neuroimmunomodulation by Macrophage Migration Inhibitory Factor (MIF)
BUCALA R. Neuroimmunomodulation by Macrophage Migration Inhibitory Factor (MIF). Annals Of The New York Academy Of Sciences 1998, 840: 74-82. PMID: 9629239, DOI: 10.1111/j.1749-6632.1998.tb09551.x.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorImmune responseRole of MIFInhibitory factorT cell cytokine productionAnti-inflammatory propertiesAnterior pituitary cellsT-lymphocyte factorSteroid therapyLymph nodesCytokine productionImmunosuppressive effectsInflammatory conditionsGlucocorticoid actionT cellsFirst cytokineInflammatory sitesTherapeutic strategiesProinflammatory stimuliPituitary cellsImmune systemLymphocyte factorsGlucocorticoidsInhibitory effectInvolvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis
Leech M, Metz C, Santos L, Peng T, Holdsworth S, Bucala R, Morand E. Involvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis. Arthritis & Rheumatism 1998, 41: 910-917. PMID: 9588744, DOI: 10.1002/1529-0131(199805)41:5<910::aid-art19>3.0.co;2-e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArthritis, ExperimentalBlotting, WesternCell CountCells, CulturedDose-Response Relationship, DrugEdemaEnzyme-Linked Immunosorbent AssayImmunohistochemistryMacrophage Migration-Inhibitory FactorsMacrophagesMaleNeutralization TestsRatsRats, Sprague-DawleySynovial MembraneConceptsMacrophage migration inhibitory factorRat adjuvant arthritisAdjuvant arthritisMigration inhibitory factorSynovial macrophagesRat synoviumLevels of MIFRole of MIFInhibitory factorMonoclonal antibodiesArthritis clinical scoresT cell accumulationDose-dependent inhibitionEnzyme-linked immunosorbentMIF treatmentAdjuvant injectionMAb treatmentClinical scoresED-1Leukocyte numbersSpecific monoclonal antibodiesClinical aspectsT cellsArthritisMacrophage activationRole of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes
Sherry B, Zybarth G, Alfano M, Dubrovsky L, Mitchell R, Rich D, Ulrich P, Bucala R, Cerami A, Bukrinsky M. Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 1758-1763. PMID: 9465090, PMCID: PMC19180, DOI: 10.1073/pnas.95.4.1758.Peer-Reviewed Original ResearchConceptsHIV-1 infectionT lymphocytesPrimary peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsHIV-1 virionsCyclophilin ACytokine-like mannerEarly eventsMononuclear cellsHIV therapyT cellsHIV-1Viral infectionViral infectivityInfectionCsA analoguesCellular receptorsLymphocytesCypAReceptorsCellsCell surfaceCD4Peptidyl-prolyl cis-trans isomerase activity
1997
Macrophage migration inhibitory factor: a regulator of glucocorticoid activity with a critical role in inflammatory disease
Donnelly S, Bucala R. Macrophage migration inhibitory factor: a regulator of glucocorticoid activity with a critical role in inflammatory disease. Trends In Molecular Medicine 1997, 3: 502-507. PMID: 9430786, DOI: 10.1016/s1357-4310(97)01133-7.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorAcute respiratory distress syndromeRelease of MIFFunction of MIFEfficacy of glucocorticoidsRespiratory distress syndromeAnti-inflammatory effectsMigration inhibitory factorEffects of glucocorticoidsAnterior pituitary glandARDS pathogenesisDistress syndromeGlucocorticoid activityInflammatory diseasesT cellsFirst cytokineInflammatory sitesPituitary glandInhibitory factorGlucocorticoidsCritical rolePhysiological responsesCytokinesPathogenesisSyndromeThe peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ
Chesney J, Bacher M, Bender A, Bucala R. The peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6307-6312. PMID: 9177213, PMCID: PMC21045, DOI: 10.1073/pnas.94.12.6307.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsAntigens, CDCell DifferentiationCell MovementCells, CulturedCicatrixCoculture TechniquesCrosses, GeneticFemaleFibroblastsFlow CytometryHIVHIV Core Protein p24HIV Envelope Protein gp120HLA-DR AntigensHumansImmunophenotypingLymphocyte ActivationMaleMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred DBANeutralization TestsSkinT-LymphocytesConceptsNaive T cellsAntigen presentationT cellsHuman fibrocytesDistinct cell surface phenotypePrime naive T cellsPotent antigen-presenting cellsMajor histocompatability complex (MHC) moleculesAdhesion molecules CD11aAntigen-specific immunityProximal lymph nodesPeripheral blood fibrocytesAntigen-presenting cellsCostimulatory molecules CD80T cell proliferationCell surface phenotypeBlood-borne cellsHIV protein p24Dendritic cellsLymph nodesBlood fibrocytesPotent APCsTissue injurySurface phenotypeCutaneous injuryRole of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response
Metz C, Bucala R. Role of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response. Advances In Immunology 1997, 66: 197-223. PMID: 9328642, DOI: 10.1016/s0065-2776(08)60598-2.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorT cellsImmune responseInhibitory factorAnti-inflammatory effectsInflammatory disease statesAnterior pituitary glandSeptic shockMIF actionMIF proteinInflammatory responseGlucocorticoid actionImmune systemPituitary glandCorticotrophic cellsGlucocorticoidsInhibitory effectDisease statesBaseline statePotential roleMacrophagesCellsResponseArthritis
1996
MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid‐induced regulator of the immune response
Bucala R. MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid‐induced regulator of the immune response. The FASEB Journal 1996, 10: 1607-1613. PMID: 9002552, DOI: 10.1096/fasebj.10.14.9002552.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorImmune responseSecretion of MIFMigration inhibitory factorAnterior pituitary cellsImmune cell activationT-lymphocyte productsProfound inhibitory effectFirst cytokine activitiesCounterregulatory hormonesLymph nodesCytokine productionImmunosuppressive effectsGlucocorticoid actionLymphocyte productsT cellsPituitary hormonesInflammatory sitesCytokine activityPituitary cellsSteroid concentrationsCell activationImmune systemInhibitory factorInhibitory effectDelayed‐type hypersensitivity mediates Bowman's capsule rupture in Tamm—Horsfall protein‐induced tubulointerstitial nephritis in the rat
Qing S, NIKOLIC‐PATERSON D, ATKINS R, BACHER M, BUCALA R, Hui Y. Delayed‐type hypersensitivity mediates Bowman's capsule rupture in Tamm—Horsfall protein‐induced tubulointerstitial nephritis in the rat. Nephrology 1996, 2: 417-427. DOI: 10.1111/j.1440-1797.1996.tb00123.x.Peer-Reviewed Original ResearchBowman's capsule ruptureTamm-Horsfall proteinCapsule ruptureBowman's capsuleT cellsCapsule integrityTubulointerstitial nephritisWeek 26Immune responseSkin delayed-type hypersensitivity responseDelayed-type hypersensitivity responseCytokine macrophage migration inhibitory factorMacrophage migration inhibitory factorIntercellular adhesion molecule-1Interstitial T cellsDelayed-type hypersensitivityCellular immune responsesHumoral immune responseMigration inhibitory factorAdhesion molecule-1Site of ruptureIntact Bowman's capsuleRat crescentic glomerulonephritisGroups of animalsMacrophage-like cells