2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Membrane StructuresClathrinEndocytosisHumansKineticsMicroscopy, Fluorescence
2010
Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nature Cell Biology 2010, 12: 902-908. PMID: 20729836, PMCID: PMC3338250, DOI: 10.1038/ncb2094.Peer-Reviewed Original ResearchActinsAcyltransferasesAdenosine TriphosphateAnimalsAntibodiesBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCattleCell LineCell MembraneCell Membrane StructuresCell-Free SystemClathrinCoated Pits, Cell-MembraneCytosolDynaminsEndocytosisFatty Acid-Binding ProteinsFibroblastsGuanosine 5'-O-(3-Thiotriphosphate)Guanosine TriphosphateHumansImaging, Three-DimensionalMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescenceModels, BiologicalPotoroidaeRatsReceptors, TransferrinThiazolidines
2004
Visualization of plasma membrane compartmentalization with patterned lipid bilayers
Wu M, Holowka D, Craighead HG, Baird B. Visualization of plasma membrane compartmentalization with patterned lipid bilayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13798-13803. PMID: 15356342, PMCID: PMC518836, DOI: 10.1073/pnas.0403835101.Peer-Reviewed Original ResearchConceptsLipid raftsLeaflet componentsPlasma membrane compartmentalizationReceptor clustersMembrane structural organizationLipid bilayersFcepsilon receptor ITyrosine phosphorylation activityMembrane compartmentalizationLyn kinaseInner leafletPhosphorylation activityActin polymerizationCellular componentsStructural organizationCell receptorRaftsCell activationReceptor IMast cell receptorsStructural reorganizationKinaseUnique insightsBilayersProtein