2023
The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection
McCollum C, Didychuk A, Liu D, Murray-Nerger L, Cristea I, Glaunsinger B. The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection. PLOS Pathogens 2023, 19: e1011163. PMID: 37068108, PMCID: PMC10138851, DOI: 10.1371/journal.ppat.1011163.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusGene expressionViral transcriptionKSHV gene expressionCellular RNA polymerasesHerpesvirus gene expressionTranscriptional activator proteinViral DNA packaging motorsPotential gene expressionTranscriptional activation complexLate gene expressionDNA packaging motorRNA polymeraseProximity labelingHost transcriptionSiRNA screenSarcoma-associated herpesvirusLate genesTranscription complexLate proteinsCatalytic subunitActivator proteinPackaging motorORF29Viral packaging
2020
The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
Castañeda A, Didychuk A, Louder R, McCollum C, Davis Z, Nogales E, Glaunsinger B. The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription. PLOS Pathogens 2020, 16: e1008843. PMID: 32886723, PMCID: PMC7498053, DOI: 10.1371/journal.ppat.1008843.Peer-Reviewed Original ResearchConceptsTATA box-binding proteinRNA polymerase IIN-terminal domainPol IIPolymerase IICellular TATA box binding proteinHost RNA polymerase IIRecruitment of RNA polymerase IIGene transcriptionLate gene transcriptionPol II recruitmentProtein interaction studiesProtein-protein contactsC-terminal domainEukaryotic transcriptionPolymerase recruitmentHuman cytomegalovirusPreinitiation complexHost transcriptionRNA PolLate genesMicroscopy-based imagingKaposi's sarcoma-associated virusTranscriptional activityPromoter recognition