2022
Better late than never: A unique strategy for late gene transcription in the beta- and gammaherpesviruses
Dremel S, Didychuk A. Better late than never: A unique strategy for late gene transcription in the beta- and gammaherpesviruses. Seminars In Cell And Developmental Biology 2022, 146: 57-69. PMID: 36535877, PMCID: PMC10101908, DOI: 10.1016/j.semcdb.2022.12.001.Peer-Reviewed Original ResearchConceptsViral transcriptional activityViral preinitiation complexPol IITranscription of late genesGene transcriptionCellular RNA polymerase IITATA-binding proteinRNA polymerase IIModified TATA boxCis-acting elementsSubfamily of herpesvirusesPolymerase IITATA boxPreinitiation complexConsensus sequenceLate genesTranscriptional activityGene promoterLytic replicationGenesTemporal cascadeTranscriptionViral mimicPolPromoter
2020
The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
Castañeda A, Didychuk A, Louder R, McCollum C, Davis Z, Nogales E, Glaunsinger B. The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription. PLOS Pathogens 2020, 16: e1008843. PMID: 32886723, PMCID: PMC7498053, DOI: 10.1371/journal.ppat.1008843.Peer-Reviewed Original ResearchConceptsTATA box-binding proteinRNA polymerase IIN-terminal domainPol IIPolymerase IICellular TATA box binding proteinHost RNA polymerase IIRecruitment of RNA polymerase IIGene transcriptionLate gene transcriptionPol II recruitmentProtein interaction studiesProtein-protein contactsC-terminal domainEukaryotic transcriptionPolymerase recruitmentHuman cytomegalovirusPreinitiation complexHost transcriptionRNA PolLate genesMicroscopy-based imagingKaposi's sarcoma-associated virusTranscriptional activityPromoter recognitionConserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34
Didychuk A, Castañeda A, Kushnir L, Huang C, Glaunsinger B. Conserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34. Journal Of Virology 2020, 94: 10.1128/jvi.01299-19. PMID: 31578296, PMCID: PMC6955276, DOI: 10.1128/jvi.01299-19.Peer-Reviewed Original ResearchConceptsViral preinitiation complexKaposi's sarcoma-associated herpesvirusC-terminal domainCysteine-rich motifHost transcription machineryPreinitiation complexGene promoterTranscription machinerySarcoma-associated herpesvirusLate genesGene transcriptionViral late gene expressionZinc finger motifsLate gene transcriptionSequence-specific bindingTranscriptional regulatory activityLate gene expressionLate gene promotersInfectious virionsProduction of capsid proteinsRelease of infectious virionsViral replication cycleFinger motifPromoter occupancySequence-specific