2019
RIG-I Recognition of RNA Targets: The Influence of Terminal Base Pair Sequence and Overhangs on Affinity and Signaling
Ren X, Linehan MM, Iwasaki A, Pyle AM. RIG-I Recognition of RNA Targets: The Influence of Terminal Base Pair Sequence and Overhangs on Affinity and Signaling. Cell Reports 2019, 29: 3807-3815.e3. PMID: 31851914, DOI: 10.1016/j.celrep.2019.11.052.Peer-Reviewed Original ResearchConceptsRNA moleculesRIG-I activationBase pair sequenceHost RNA moleculesViral RNA moleculesRIG-I recognitionMolecular basisRNA variantsRNA targetsPair sequenceHuman cellsBase pairsImmune receptorsMechanisms of evasionTerminal base pairsLigand affinityWhole animalInterferon responseDeadly pathogenRNA therapeuticsMarburg virusCellsOverhangMoleculesSignaling
2013
Defining the functional determinants for RNA surveillance by RIG‐I
Kohlway A, Luo D, Rawling DC, Ding SC, Pyle AM. Defining the functional determinants for RNA surveillance by RIG‐I. EMBO Reports 2013, 14: 772-779. PMID: 23897087, PMCID: PMC3790051, DOI: 10.1038/embor.2013.108.Peer-Reviewed Original ResearchConceptsMelanoma differentiation-associated gene 5Robust ATPase activityDuplex RNA substrateMinimal functional unitATPase activityRetinoic acid-inducible geneInnate immune machineryAcid-inducible geneRNA surveillanceDifferentiation-associated gene 5RNA substratesIntracellular RNA sensorsDuplex RNARNA complexRNA targetsGene 5RNA virusesDistinct conformationsRNA sensorsDsRNA complexImmune machineryRNA duplexesInterferon responseFunctional determinantsFunctional units
2000
[10] Using DNAzylnes to cut, process, and map RNA molecules for structural studies or modification
Pyle AM, Chu VT, Jankowsky E, Boudvillain M. [10] Using DNAzylnes to cut, process, and map RNA molecules for structural studies or modification. Methods In Enzymology 2000, 317: 140-146. PMID: 10829278, DOI: 10.1016/s0076-6879(00)17012-0.Peer-Reviewed Original ResearchConceptsStructural studiesTypes of DNAzymeTemplate-directed ligationDNAzyme reactionDNAzyme moleculesSimple DNA moleculesDNAzymeDivalent ionsAnalytical scaleMoleculesDNA moleculesAddition of Mg2Crystallographic applicationsRNA targetsRNA moleculesCatalytic coreIonsLong RNAsReactionModificationDifferent applicationsInexpensive wayRNA fragmentsMg2Incorporation