2021
MRTFA: A critical protein in normal and malignant hematopoiesis and beyond
Reed F, Larsuel ST, Mayday MY, Scanlon V, Krause DS. MRTFA: A critical protein in normal and malignant hematopoiesis and beyond. Journal Of Biological Chemistry 2021, 296: 100543. PMID: 33722605, PMCID: PMC8079280, DOI: 10.1016/j.jbc.2021.100543.Peer-Reviewed Original ResearchConceptsMalignant hematopoiesisActin cytoskeleton dynamicsCritical cellular functionsResponse factorSerum response factorTranscription factor ACellular rolesImmediate early genesProtein partnersTranscriptional regulationCytoskeleton dynamicsCellular functionsTranscriptional targetsTranscription factorsCytoskeletal proteinsCritical proteinsMRTFAEarly genesCell typesChromosomal translocationsHematopoietic cellsCell growthFactor AHematopoiesisMuscle cells
2018
The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification
Lu YC, Sanada C, Xavier-Ferrucio J, Wang L, Zhang PX, Grimes HL, Venkatasubramanian M, Chetal K, Aronow B, Salomonis N, Krause DS. The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell Reports 2018, 25: 2083-2093.e4. PMID: 30463007, PMCID: PMC6336197, DOI: 10.1016/j.celrep.2018.10.084.Peer-Reviewed Original ResearchMeSH KeywordsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCell CycleCell LineageGene Expression RegulationGene Regulatory NetworksHEK293 CellsHigh-Throughput Nucleotide SequencingHumansMegakaryocyte-Erythroid Progenitor CellsProto-Oncogene Proteins c-mycReproducibility of ResultsSignal TransductionTranscription, GeneticTumor Suppressor Protein p53ConceptsMegakaryocytic-erythroid progenitorsCommon myeloid progenitorsTranscription factorsCell cycleSingle-cell RNA sequencingRegulatory transcription factorsMegakaryocyte-erythroid progenitorsCell cycle regulatorsCell cycle activationFate specificationLineage specificationE lineageMalignant disease statesGenetic manipulationRNA sequencingE progenitorsErythroid maturationCycle regulatorsDifferential expressionHuman cellsHealthy human cellsCycle activationMegakaryocyte progenitorsMolecular signaturesMyeloid progenitors
2007
Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation
Ma X, Renda MJ, Wang L, Cheng EC, Niu C, Morris SW, S. AS, Krause DS. Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Molecular And Cellular Biology 2007, 27: 3056-3064. PMID: 17283045, PMCID: PMC1899951, DOI: 10.1128/mcb.01339-06.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBasic Helix-Loop-Helix Transcription FactorsCell NucleusCHO CellsCricetinaeCricetulusDNA-Binding ProteinsDrosophila ProteinsGene Expression ProfilingHomeodomain ProteinsImmunoglobulin J Recombination Signal Sequence-Binding ProteinMiceMolecular Sequence DataMyeloid CellsMyelopoiesisNuclear ProteinsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReceptors, NotchRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsTranscription Factor HES-1Transcription, GeneticTranscriptional ActivationConceptsN-terminusPromoter activityMyeloid differentiationCell linesCell type-specific mannerMyeloid precursor cell linePrimary murine cellsType-specific mannerDominant negative effectStimulation of NotchHematopoietic cell linesHuman erythroleukemia cellsPrecursor cell lineMurine cell linesHematopoietic stem cellsTranscriptional activationHes1 transcriptionRNA interferenceErythroleukemia cellsFusion proteinHes1 promoter activityMurine cellsFusion partnerHematopoietic cellsRBM15
2000
Regulation of CD34 transcription by Sp1 requires sites upstream and downstream of the transcription start site
Taranenko N, Krause D. Regulation of CD34 transcription by Sp1 requires sites upstream and downstream of the transcription start site. Experimental Hematology 2000, 28: 974-984. PMID: 10989198, DOI: 10.1016/s0301-472x(00)00492-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Antigens, NuclearBinding SitesDNADNA HelicasesDNA MethylationDNA ProbesDNA-Binding ProteinsGene Expression RegulationGuanineKu AutoantigenMiceNuclear ProteinsPromoter Regions, GeneticSp1 Transcription FactorTranscription FactorsTranscription, GeneticTransfectionTumor Cells, CulturedUntranslated RegionsConceptsTranscription start sitePromoter activityCD34 promoterStart siteDrosophila S2 cellsHematopoietic cellsTranscription factor bindsDNA binding sitesElectromobility shift assaysSequence-specific mannerS2 cellsTranscriptional regulationMolecular regulationMethylation interferenceDeletion analysisEarly hematopoiesisNuclear proteinsRegulatory regionsFactor bindsShift assaysGene expressionUntranslated regionHematopoietic stemSp1CD34 transcription
1998
Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines.
Lawson ND, Krause DS, Berliner N. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Experimental Hematology 1998, 26: 1178-85. PMID: 9808058.Peer-Reviewed Original ResearchConceptsNeutrophil differentiationDominant-negative retinoic acid receptor alphaCell linesNormal neutrophil differentiationRetinoic acid receptor alphaCD11b/CD18Macrophage colony-stimulating factorDownregulation of CD34Granulocyte/macrophage colony-stimulating factorTrans retinoic acidAcid receptor alphaColony-stimulating factorNeutrophil gelatinaseGranulocytic differentiation programReceptor alphaSerum-free mediumAppropriate cytokinesMyeloid lineageRetinoic acidInduces expressionLeukemic myelopoiesisHL60MyelopoiesisNeutrophilic differentiationDifferentiation programCD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb.
Krause DS, Mucenski ML, Lawler AM, May WS. CD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb. Experimental Hematology 1998, 26: 1086-92. PMID: 9766450.Peer-Reviewed Original ResearchConceptsC-MybDevelopmental hematopoiesisES cellsPromoter activityHematopoietic cellsD3 embryonic stem cellsStage-specific mannerES cell differentiationEmbryonic stem cellsPrimitive hematopoietic cellsDefinitive hematopoiesisBone marrow stemCell surface glycoproteinBlood islandsEmbryoid bodiesEndothelial cellsTransient transfectionCell differentiationEmbryosStem cellsProgenitor cellsYolk sacHematopoiesisNonhematopoietic cellsCD34 expression
1997
Regulation of CD34 expression in differentiating M1 cells.
Krause DS, Kapadia SU, Raj NB, May WS. Regulation of CD34 expression in differentiating M1 cells. Experimental Hematology 1997, 25: 1051-61. PMID: 9293902.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntigens, CD34Base SequenceBinding SitesCell DifferentiationCells, CulturedDNA-Binding ProteinsDown-RegulationGene Expression RegulationGene Expression Regulation, DevelopmentalGene Expression Regulation, NeoplasticHematopoiesisLeukemia, MyeloidMiceMolecular Sequence DataNuclear ProteinsRNA, MessengerTranscription, GeneticConceptsTranscription initiation siteUntranslated regionPromoter activityHematopoietic stemCell type-specific expressionSecondary structureTATA-less promoterPromoter-luciferase reporter constructsFull promoter activityUpstream genomic DNAProgenitor cellsTranslation start siteMature blood cellsType-specific expressionOptimal promoter activityExtensive secondary structureP1 nuclease digestionCell-specific factorsTranscriptional initiationGene regulationTranscription factorsConsensus sitesStart siteRegulatory elementsTATA element