2022
PUMILIO proteins promote colorectal cancer growth via suppressing p21
Gong Y, Liu Z, Yuan Y, Yang Z, Zhang J, Lu Q, Wang W, Fang C, Lin H, Liu S. PUMILIO proteins promote colorectal cancer growth via suppressing p21. Nature Communications 2022, 13: 1627. PMID: 35338151, PMCID: PMC8956581, DOI: 10.1038/s41467-022-29309-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological PhenomenaColorectal NeoplasmsMiceMice, KnockoutRNA, MessengerRNA-Binding ProteinsConceptsColorectal cancerAOM/DSS modelIntestine-specific knockoutColitis-associated cancerHuman CRC cellsOrthotopic colon cancer modelColorectal cancer growthG1/S transitionHuman colorectal cancerColorectal tumor growthColon cancer modelCancer cell growthCRC progressionCRC cellsIntravenous injectionTherapeutic targetCancer growthCancer modelTumor growthSignificant decreaseS transitionDirect targetP21 mRNACancerDSS modelPrecision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies
Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon MD, Neugebauer KM, Tebaldi T, Halene S. Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies. Molecular Cell 2022, 82: 1107-1122.e7. PMID: 35303483, PMCID: PMC8988922, DOI: 10.1016/j.molcel.2022.02.025.Peer-Reviewed Original Research
2021
Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs
Wang C, Lin H. Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs. Genome Biology 2021, 22: 27. PMID: 33419460, PMCID: PMC7792047, DOI: 10.1186/s13059-020-02221-x.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsRole of piRNAsPIWI-piRNA pathwayRNA-binding proteinSmall noncoding RNAsGermline mRNAsGermline developmentNoncoding RNAsRegulatory relationshipsGerm cellsRNAProteinTransposonMajor classesRNA levelsRecent studiesExpressionGenomeSubfamiliesGermlineLncRNAsMajor constituentsMRNARegulation
2020
Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis
Uyhazi KE, Yang Y, Liu N, Qi H, Huang XA, Mak W, Weatherbee SD, de Prisco N, Gennarino VA, Song X, Lin H. Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 7851-7862. PMID: 32198202, PMCID: PMC7148564, DOI: 10.1073/pnas.1916471117.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsTarget messenger RNAsPumilio proteinsPUM proteinsMessenger RNAEssential functionsStem cell maintenanceDistinct regulatory mechanismsEmbryonic day 8.5ESC pluripotencyTranslational regulatorPluripotency genesGene regulationEarly embryogenesisDifferentiation genesPosttranscriptional levelHigh homologyMRNA stabilityRegulatory mechanismsDouble mutant micePluripotencyDay 8.5Morula stagePluripotency markersEmbryogenesis
2017
Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins
Zhang M, Chen D, Xia J, Han W, Cui X, Neuenkirchen N, Hermes G, Sestan N, Lin H. Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins. Genes & Development 2017, 31: 1354-1369. PMID: 28794184, PMCID: PMC5580656, DOI: 10.1101/gad.298752.117.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCytoplasmDentate GyrusFemaleFragile X Mental Retardation ProteinGene Expression Regulation, DevelopmentalGene Knockout TechniquesGene SilencingLearning DisabilitiesMaleMemory DisordersMiceNeural Stem CellsNeurogenesisNeuronsRNA, MessengerRNA-Binding ProteinsStem CellsConceptsPost-transcriptional regulationPost-transcriptional regulatorsNeural stem cellsTarget mRNAsMost target mRNAsRNA-dependent interactionCross-linking immunoprecipitationThousands of mRNAsMental retardation proteinPUM proteinsPumilio proteinsPumilio 1Mouse neurogenesisMammalian neurogenesisPerinatal apoptosisPUM1PUM2Stem cellsProteinCommon targetMRNARegulatorNeurogenesisCell compositionRegulation
2016
An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline1
Mak W, Fang C, Holden T, Dratver MB, Lin H. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline1. Biology Of Reproduction 2016, 94: 134, 1-11. PMID: 27170441, PMCID: PMC4946805, DOI: 10.1095/biolreprod.115.137497.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA-Binding ProteinsFemaleMice, TransgenicNuclear ProteinsOocytesOogenesisOvarian FollicleReproductionRNA-Binding ProteinsConceptsPumilio 1PUF proteinsLate meiotic prophase IPumilio/FBF (PUF) proteinReproductive competencyPrimordial follicle poolFemale germ cell developmentMammalian germ cellsPrimordial folliculogenesisFemale reproductive competenceMeiotic prophase IGerm cell developmentFBF proteinsGermline establishmentTranslational regulatorTranslational regulationProphase IDiplotene stageCell developmentGerm cellsFollicle poolImportant roleDetectable functionMammalsReproductive competenceThe Role of PIWIL4, an Argonaute Family Protein, in Breast Cancer*
Wang Z, Liu N, Shi S, Liu S, Lin H. The Role of PIWIL4, an Argonaute Family Protein, in Breast Cancer*. Journal Of Biological Chemistry 2016, 291: 10646-10658. PMID: 26957540, PMCID: PMC4865913, DOI: 10.1074/jbc.m116.723239.Peer-Reviewed Original ResearchConceptsP-element-induced wimpy testisPIWI-interacting RNAsPIWI proteinsMDA-MB-231 cellsArgonaute family proteinsGermline developmentDiverse organismsWimpy testisFamily proteinsProteome analysisClass II proteinsPIWIL4Potential therapeutic targetStem cellsProteinMHC class II proteinsMigration abilityRNATherapeutic targetPIWIL4 expressionCancer tissuesBreast cancer tissuesCellsKey roleBiogenesis
2013
Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline
Saxe JP, Chen M, Zhao H, Lin H. Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. The EMBO Journal 2013, 32: 1869-1885. PMID: 23714778, PMCID: PMC3981179, DOI: 10.1038/emboj.2013.121.Peer-Reviewed Original ResearchConceptsPrimary piRNA biogenesisPiRNA biogenesisKH domain-containing proteinPiRNA biogenesis pathwayPing-pong cycleDomain-containing proteinsMature piRNAsPIWI proteinsBiogenesis pathwayMitochondrial proteinsEpigenetic programmingNuclear localizationCytoplasmic localizationZygotene stageBiogenesisTDRKHArginine residuesMeiotic arrestMIWI2MIWIDistinct populationsProteinMutantsGermlineRNA
2012
Pumilio 1 Suppresses Multiple Activators of p53 to Safeguard Spermatogenesis
Chen D, Zheng W, Lin A, Uyhazi K, Zhao H, Lin H. Pumilio 1 Suppresses Multiple Activators of p53 to Safeguard Spermatogenesis. Current Biology 2012, 22: 420-425. PMID: 22342750, PMCID: PMC3449084, DOI: 10.1016/j.cub.2012.01.039.Peer-Reviewed Original Research
2011
PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition
Liu L, Qi H, Wang J, Lin H. PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. Development 2011, 138: 1863-1873. PMID: 21447556, PMCID: PMC3074456, DOI: 10.1242/dev.059287.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedArgonaute ProteinsCarrier ProteinsDEAD-box RNA HelicasesDNA Transposable ElementsDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGene SilencingGerm CellsMaleModels, BiologicalMutagenesis, InsertionalPeptide Initiation FactorsProtein BindingRibonucleoproteinsRNA-Binding ProteinsRNA-Induced Silencing ComplexConceptsPiRNA pathway componentsPIWI-interacting RNAsArgonaute 3PiRNA pathwayPIWI proteinsTransposon activationPathway componentsPIWI protein AubergineTudor domain proteinsP-body componentsN-terminal domainNuage componentsPiRNA mutantsTransposon controlGermline developmentTudor domainMutant ovariesArginine methyltransferaseGermline genomeEpigenetic regulationPerinuclear structuresNuageAdult ovariesArginine residuesFunctional interactionGenome‐Wide Studies Reveal That Lin28 Enhances the Translation of Genes Important for Growth and Survival of Human Embryonic Stem Cells
Peng S, Chen L, Lei X, Yang L, Lin H, Carmichael GG, Huang Y. Genome‐Wide Studies Reveal That Lin28 Enhances the Translation of Genes Important for Growth and Survival of Human Embryonic Stem Cells. Stem Cells 2011, 29: 496-504. PMID: 21425412, DOI: 10.1002/stem.591.Peer-Reviewed Original ResearchConceptsRNA helicase AHuman embryonic stem cellsEmbryonic stem cellsLin28-dependent stimulationTranslation of genesStem cellsLet-7 microRNADominant negative inhibitorPolysome profilingGenes ImportantRibosomal proteinsCellular mRNAsTarget genesDeep sequencingReporter analysisMetabolic enzymesLin28Cell growthExpression levelsGenesTranslationCellsGrowthImmunoprecipitationMicroRNAs
2009
The Biogenesis and Function of PIWI Proteins and piRNAs: Progress and Prospect
Thomson T, Lin H. The Biogenesis and Function of PIWI Proteins and piRNAs: Progress and Prospect. Annual Review Of Cell And Developmental Biology 2009, 25: 355-376. PMID: 19575643, PMCID: PMC2780330, DOI: 10.1146/annurev.cellbio.24.110707.175327.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsGermline stem cell maintenanceStem cell maintenanceSmall noncoding RNAsGermline determinationAGO proteinsGermline developmentMetazoan speciesPiRNA speciesPIWI subfamiliesSmall RNAsIntergenic sequencesPosttranscriptional regulationCell maintenanceRNA precursorsNoncoding RNAsPiwi familyDiverse functionsDiverse tissuesRNANovel mechanismProteinBiogenesisSpecies
2006
Sex-lethal is a target of Bruno-mediated translational repression in promoting the differentiation of stem cell progeny during Drosophila oogenesis
Wang Z, Lin H. Sex-lethal is a target of Bruno-mediated translational repression in promoting the differentiation of stem cell progeny during Drosophila oogenesis. Developmental Biology 2006, 302: 160-168. PMID: 17067567, PMCID: PMC1904479, DOI: 10.1016/j.ydbio.2006.09.016.Peer-Reviewed Original ResearchConceptsBruno response elementCystoblast differentiationTranslational repressionElectrophoresis mobility shift assaysGermline stem cellsPotential mRNA targetsMobility shift assaysStem cell progenySex-lethalDrosophila ovaryDrosophila oogenesisMutant phenotypeBioinformatics approachMRNA targetsShift assaysType RNACDNA constructsUntranslated regionCell progenyResponse elementStem cellsNovel targetDifferentiationRepressionMS11
2005
Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation
Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Current Biology 2005, 15: 171-178. PMID: 15668175, DOI: 10.1016/j.cub.2005.01.005.Peer-Reviewed Original ResearchConceptsGermline stem cellsPiwi functionDrosophila ovarian germline stem cellsOvarian germline stem cellsStem Cell Self-RenewalFunction of PiwiDifferentiated daughter cellsCell Self-RenewalGerm cell divisionBam proteinCystoblast differentiationBam expressionMutant ovariesNiche signalsDouble mutantDaughter cellsDifferentiation genesPiwiCell divisionNiche cellsRegulatory relationshipsSelf-RenewalGerm cellsMutantsCystoblasts
2004
Nanos Maintains Germline Stem Cell Self-Renewal by Preventing Differentiation
Wang Z, Lin H. Nanos Maintains Germline Stem Cell Self-Renewal by Preventing Differentiation. Science 2004, 303: 2016-2019. PMID: 14976263, DOI: 10.1126/science.1093983.Peer-Reviewed Original ResearchConceptsGermline stem cellsPrimordial germ cellsGermline cystsStem Cell Self-RenewalTranslational repressor NanosCell Self-RenewalStem cellsDrosophila ovaryGene regulationExtrinsic signalingPrecocious entrySelf-RenewalGerm cellsCell typesDifferentiation factorCellsCystoblastsOogenesisSignalingDifferentiationRegulationOvariesTranslation