2023
Apoptosis recognition receptors regulate skin tissue repair in mice
Justynski O, Bridges K, Krause W, Forni M, Phan Q, Sandoval-Schaefer T, Carter K, King D, Hsia H, Gazes M, Vyce S, Driskell R, Miller-Jensen K, Horsley V. Apoptosis recognition receptors regulate skin tissue repair in mice. ELife 2023, 12: e86269. PMID: 38127424, PMCID: PMC10735221, DOI: 10.7554/elife.86269.Peer-Reviewed Original Research
2020
A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing
Dash BC, Setia O, Gorecka J, Peyvandi H, Duan K, Lopes L, Nie J, Berthiaume F, Dardik A, Hsia HC. A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing. Cells 2020, 9: 966. PMID: 32295218, PMCID: PMC7226960, DOI: 10.3390/cells9040966.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsSecretory functionHuman-induced pluripotent stem cellsParacrine secretionMuscle cellsCollagen fibrillar densitiesSecretory factorsVascular regenerationWound healingTissue-engineered vascular graftsHealingVascular graftsStem cellsPluripotent stem cellsCollagen scaffoldsScaffold propertiesScaffold materialsCellsPromising strategyDisease modelingFibrillar densityRegenerative wound healingCytokinesGraftInduced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing
Gorecka J, Gao X, Fereydooni A, Dash BC, Luo J, Lee SR, Taniguchi R, Hsia HC, Qyang Y, Dardik A. Induced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing. Regenerative Medicine 2020, 15: 1277-1293. PMID: 32228292, PMCID: PMC7304438, DOI: 10.2217/rme-2019-0086.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsMuscle cellsDiabetic wound healingWound healingPro-angiogenic cytokinesMurine AdiposeStem cellsType macrophagesCollagen scaffoldsCultured mediumM2-type macrophagesCellsNumber of totalNew candidatesAngiogenesisNude miceDiabetic woundsPromising new candidateScaffoldsHealingCytokinesExpressionSecreteWoundsAdipose
2019
Mouse Model of Pressure Ulcers After Spinal Cord Injury.
Kumar S, Tan Y, Yarmush ML, Dash BC, Hsia HC, Berthiaume F. Mouse Model of Pressure Ulcers After Spinal Cord Injury. Journal Of Visualized Experiments 2019 PMID: 30907872, DOI: 10.3791/58188.Peer-Reviewed Original ResearchConceptsSpinal cord injuryLevel of SCIPressure ulcersSCI miceCord injuryMouse modelComplete spinal cord injuryImpact of SCITraumatic spinal cord injuryComplete spinal cord transectionSpinal cord transectionAdult male miceRelevant mouse modelCord transectionIschemic areaMale miceTissue edemaPU developmentTherapeutic approachesAnimal modelsBony prominencesSuch woundsMiceSoft tissueHealing
2018
Myofibroblast proliferation and heterogeneity are supported by macrophages during skin repair
Shook BA, Wasko RR, Rivera-Gonzalez GC, Salazar-Gatzimas E, López-Giráldez F, Dash BC, Muñoz-Rojas AR, Aultman KD, Zwick RK, Lei V, Arbiser JL, Miller-Jensen K, Clark DA, Hsia HC, Horsley V. Myofibroblast proliferation and heterogeneity are supported by macrophages during skin repair. Science 2018, 362 PMID: 30467144, PMCID: PMC6684198, DOI: 10.1126/science.aar2971.Peer-Reviewed Original ResearchConceptsDifferential gene expressionAdipocyte precursorsExtracellular matrix moleculesGene expressionTransplantation assaysMatrix moleculesFactor C.Factor 1Insulin-like growth factor-1Cell populationsTissue resilienceDistinct subpopulationsGrowth factor-1Profibrotic cellsTissue repairMultiple mouse modelsECM depositionSkin repairTissue dysfunctionProliferationMouse modelMyofibroblastsWoundingMacrophagesRepairImpact of Complete Spinal Cord Injury on Healing of Skin Ulcers in Mouse Models
Kumar S, Yarmush ML, Dash BC, Hsia HC, Berthiaume F. Impact of Complete Spinal Cord Injury on Healing of Skin Ulcers in Mouse Models. Journal Of Neurotrauma 2018, 35: 815-824. PMID: 29160147, DOI: 10.1089/neu.2017.5405.Peer-Reviewed Original ResearchConceptsComplete spinal cord injurySpinal cord injuryPressure ulcersSCI miceSCI groupCord injuryMouse modelControl groupImpact of SCIExcisional woundsTraumatic spinal cord injuryAlpha-smooth muscle actinAdult male miceRelevant mouse modelLower blood vessel densitySuitable animal modelBlood vessel densityFull-thickness excisional woundsDynamics of skinBacks of miceIschemic areaMale miceTissue edemaSkin ulcersAnimal models
2015
SDF‐1 liposomes promote sustained cell proliferation in mouse diabetic wounds
Olekson MA, Faulknor R, Bandekar A, Sempkowski M, Hsia HC, Berthiaume F. SDF‐1 liposomes promote sustained cell proliferation in mouse diabetic wounds. Wound Repair And Regeneration 2015, 23: 711-723. PMID: 26110250, DOI: 10.1111/wrr.12334.Peer-Reviewed Original ResearchConceptsStromal cell-derived factor-1Acellular dermisCell-derived factor-1Cell proliferationRestoration of skinChronic skin woundsImproved wound closurePersistent cell proliferationCommon complicationGranulation tissue thicknessIschemic tissueProper revascularizationDay 28Day 21Diabetic woundsSuch woundsWound closureSkin woundsDermisFactor 1Tissue thicknessWoundsProliferation
2014
The fate of internalized α5 integrin is regulated by matrix-capable fibronectin
Hsia HC, Nair MR, Corbett SA. The fate of internalized α5 integrin is regulated by matrix-capable fibronectin. Journal Of Surgical Research 2014, 191: 268-279. PMID: 25062814, PMCID: PMC4160403, DOI: 10.1016/j.jss.2014.05.084.Peer-Reviewed Original ResearchConceptsFibronectin matrix assemblyΑ5 integrinFibronectin matrixMatrix assemblySpecific lysine residuesMouse embryo fibroblast cellsEmbryo fibroblast cellsExtracellular spaceIntegrin turnoverCytoplasmic tailTissue-remodeling processesAbsence of fibronectinInternalized receptorsLysine residuesReceptor turnoverIntegrinsTissue remodelingUbiquitinationProtein levelsChinese hamsterFibroblast cellsFibronectin dimersWound repairRapid degradationFate
2004
Coregulation of Fibronectin Signaling and Matrix Contraction by Tenascin-C and Syndecan-4
Midwood KS, Valenick LV, Hsia HC, Schwarzbauer JE. Coregulation of Fibronectin Signaling and Matrix Contraction by Tenascin-C and Syndecan-4. Molecular Biology Of The Cell 2004, 15: 5670-5677. PMID: 15483051, PMCID: PMC532045, DOI: 10.1091/mbc.e04-08-0759.Peer-Reviewed Original ResearchConceptsSyndecan-4 functionsSyndecan-4Actin stress fiber formationFocal adhesion kinaseMatrix contractionStress fiber formationExtracellular matrix proteinsEfficient tissue repairEffects of tenascinProvisional matrixTissue repairHeparan sulfate proteoglycanAdhesion kinaseCell spreadingSignaling pathwaysMatrix proteinsFibronectin signalingThree-dimensional fibrinExtracellular matrixFiber formationSulfate proteoglycanCell interactionsFibroblast morphologyTenascinFibroblast response