2021
STL-seq reveals pause-release and termination kinetics for promoter-proximal paused RNA polymerase II transcripts
Zimmer JT, Rosa-Mercado NA, Canzio D, Steitz JA, Simon MD. STL-seq reveals pause-release and termination kinetics for promoter-proximal paused RNA polymerase II transcripts. Molecular Cell 2021, 81: 4398-4412.e7. PMID: 34520723, PMCID: PMC9020433, DOI: 10.1016/j.molcel.2021.08.019.Peer-Reviewed Original ResearchConceptsPause releaseRNA polymerase II transcriptsRNA polymerase II moleculesCis-acting DNA elementsTATA box-containing promotersPolymerase II transcriptsPromoter-proximal pausingCritical regulatory functionsTranscriptional regulationRNA turnoverTranscriptional controlDNA elementsTranscriptional shutdownPause sitesHyperosmotic stressRegulatory mechanismsRegulatory functionsPrinciples of regulationHormonal stimuliPausingPremature terminationTranscriptsRegulationHyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression
Rosa-Mercado NA, Zimmer JT, Apostolidi M, Rinehart J, Simon MD, Steitz JA. Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression. Molecular Cell 2021, 81: 502-513.e4. PMID: 33400923, PMCID: PMC7867636, DOI: 10.1016/j.molcel.2020.12.002.Peer-Reviewed Original ResearchConceptsWidespread transcriptional repressionTranscriptional repressionPol IIIntegrator complex subunitsRNA polymerase IIGenome-wide lossStress-induced redistributionParental genesTranscriptional outputDoG inductionPolymerase IIChIP sequencingHuman cell linesUpstream geneComplex subunitsPolyadenylation factorsTranscription profilesReadthrough transcriptsCatalytic subunitIntegrator activityCellular stressHyperosmotic stressTranscriptional levelTranscription resultsGenes
2013
Mammalian 5′-Capped MicroRNA Precursors that Generate a Single MicroRNA
Xie M, Li M, Vilborg A, Lee N, Shu MD, Yartseva V, Šestan N, Steitz JA. Mammalian 5′-Capped MicroRNA Precursors that Generate a Single MicroRNA. Cell 2013, 155: 1568-1580. PMID: 24360278, PMCID: PMC3899828, DOI: 10.1016/j.cell.2013.11.027.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsBase SequenceBiosynthetic PathwaysDEAD-box RNA HelicasesGenome-Wide Association StudyGuanosineHumansKaryopherinsMiceMicroRNAsMolecular Sequence DataReceptors, Cytoplasmic and NuclearRibonuclease IIIRNA CapsRNA Polymerase IIRNA, Small InterferingTranscription Termination, GeneticConceptsCap-binding protein eIF4EMiRNA biogenesis pathwayNuclear-cytoplasmic transportGuide strand selectionShRNA expression constructsTranscription start siteBiogenesis pathwayCytoplasmic DicerMicroprocessor complexTranscription terminationProtein eIF4EExportin-5MicroRNA precursorsMiRNA hairpinsPrimary transcriptStrand selectionGene regulatorsStart siteDicer cleavageExpression constructsSingle microRNAMiRNAsMicroRNAsPathwayMicroRNPs
1988
Four novel U RNAs are encoded by a herpesvirus
Lee S, Murthy S, Trimble J, Desrosiers R, Steitz J. Four novel U RNAs are encoded by a herpesvirus. Cell 1988, 54: 599-607. PMID: 2842058, DOI: 10.1016/s0092-8674(88)80004-7.Peer-Reviewed Original ResearchConceptsHSUR 1U RNAU RNA genesTrimethylguanosine cap structureSmall nuclear ribonucleoproteinEnd formation signalsAAUAAA polyadenylation signalBase pairing interactionsRNA genesMarmoset T lymphocytesPolyadenylation signalNuclear ribonucleoproteinCap structureEnd sequencesPerfect complementarityViral genesSnRNP particlesLow abundanceHerpesvirus saimiriRNASm determinantsGenesFormation signalEnd regionSequence
1985
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
Black D, Chabot B, Steitz J. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. Cell 1985, 42: 737-750. PMID: 2996775, DOI: 10.1016/0092-8674(85)90270-3.Peer-Reviewed Original Research
1981
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
Hendrick JP, Wolin SL, Rinke J, Lerner MR, Steitz JA. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Molecular And Cellular Biology 1981, 1: 1138-1149. PMID: 6180298, PMCID: PMC369740, DOI: 10.1128/mcb.1.12.1138.Peer-Reviewed Original ResearchConceptsLa RNAsHuman cellsRNA polymerase III transcriptsNuclear transcription systemRNA polymerase IIIUninfected mammalian cellsPolymerase III transcriptsSmall cytoplasmic ribonucleoproteinsDifferent RNA moleculesSmall ribonucleic acidsProtein complexesSmall ribonucleoproteinTransfer RNAMammalian cellsRo RNPsPolymerase IIIRNA componentCytoplasmic ribonucleoproteinRNA moleculesTranscription systemMouse cellsReconstitution experimentsRNARNA-like moleculesCell cytoplasm