2013
Identification of a New Stromal Cell Type Involved in the Regulation of Inflamed B Cell Follicles
Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli ML, Ruddle NH, Nowak J, Aurrand-Lions M, Luche H, Bajénoff M. Identification of a New Stromal Cell Type Involved in the Regulation of Inflamed B Cell Follicles. PLOS Biology 2013, 11: e1001672. PMID: 24130458, PMCID: PMC3794863, DOI: 10.1371/journal.pbio.1001672.Peer-Reviewed Original ResearchConceptsStromal cell typesB cell folliclesT cell zonesCell typesFate-mapping systemStromal cellsCellular demandB cellsLymph Node Stromal CellsSurvival signalsStromal cell subsetsB cell ablationLN stromal cellsCell zoneAdhesive substrataCell ablationCell subsetsImmune responseCellsFolliclesInflammationSubstrataRegulationTransient boundaryLymphocytes
2012
Tertiary lymphoid organ development coincides with determinant spreading of the myelin-specific T cell response
Kuerten S, Schickel A, Kerkloh C, Recks MS, Addicks K, Ruddle NH, Lehmann PV. Tertiary lymphoid organ development coincides with determinant spreading of the myelin-specific T cell response. Acta Neuropathologica 2012, 124: 861-873. PMID: 22842876, DOI: 10.1007/s00401-012-1023-3.Peer-Reviewed Original ResearchConceptsTertiary lymphoid organsExperimental autoimmune encephalomyelitisMyelin-specific T cell responseCentral nervous systemB cell aggregatesT cell responsesMultiple sclerosisB cell aggregationDeterminant spreadingB cellsCell responsesActive immune responseMyelin basic proteinLymphoid neogenesisAutoimmune encephalomyelitisMS patientsAggressive diseaseAutoimmune pathologyPatient populationLymphoid organsDisease onsetDisease progressionT cellsImmune responsePathogenic contribution
2010
The role of AIRE in human autoimmune disease
Akirav EM, Ruddle NH, Herold KC. The role of AIRE in human autoimmune disease. Nature Reviews Endocrinology 2010, 7: 25-33. PMID: 21102544, DOI: 10.1038/nrendo.2010.200.Peer-Reviewed Original ResearchMeSH KeywordsAutoimmune DiseasesHumansMutationPolyendocrinopathies, AutoimmuneT-LymphocytesTranscription FactorsPrevention of Diabetes by FTY720-Mediated Stabilization of Peri-Islet Tertiary Lymphoid Organs
Penaranda C, Tang Q, Ruddle NH, Bluestone JA. Prevention of Diabetes by FTY720-Mediated Stabilization of Peri-Islet Tertiary Lymphoid Organs. Diabetes 2010, 59: 1461-1468. PMID: 20299465, PMCID: PMC2874707, DOI: 10.2337/db09-1129.Peer-Reviewed Original ResearchConceptsTertiary lymphoid organsPancreatic lymph nodesNOD miceLymph nodesDiabetes developmentDiabetic miceLymphoid organsSpontaneous type 1 diabetesB cell compartmentalizationExit of lymphocytesNonobese diabetic (NOD) miceAge-matched miceDevelopment of diabetesPrevention of diabetesNaive T cellsType 1 diabetesB cell compartmentWeeks of ageSignificant insulitisIslet destructionTreatment withdrawalAutoimmune destructionClinical scoresAccelerated diseaseDisease progression
2007
Tertiary Lymphoid Tissues Generate Effector and Memory T Cells That Lead to Allograft Rejection
Nasr IW, Reel M, Oberbarnscheidt MH, Mounzer RH, Baddoura FK, Ruddle NH, Lakkis FG. Tertiary Lymphoid Tissues Generate Effector and Memory T Cells That Lead to Allograft Rejection. American Journal Of Transplantation 2007, 7: 1071-1079. PMID: 17359505, DOI: 10.1111/j.1600-6143.2007.01756.x.Peer-Reviewed Original ResearchConceptsTertiary lymphoid tissueWild-type allograftsMemory T cellsSecondary lymphoid organsLymphoid tissueT cellsLymphoid organsRejection processPrimary alloimmune responsesSyngeneic graft recipientsMemory immune responsesNaïve T cell activationTertiary lymphoid structuresNaive T cellsT cell activationMurine transplantation modelChronic rejectionAllograft rejectionGraft recipientsAlloimmune responseLymphoid structuresChronic inflammationSkin allograftsNaïve lymphocytesTransplantation model
2006
Synchrony of High Endothelial Venules and Lymphatic Vessels Revealed by Immunization
Liao S, Ruddle NH. Synchrony of High Endothelial Venules and Lymphatic Vessels Revealed by Immunization. The Journal Of Immunology 2006, 177: 3369-3379. PMID: 16920978, DOI: 10.4049/jimmunol.177.5.3369.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersB-LymphocytesCell Adhesion MoleculesCell CommunicationCells, CulturedDendritic CellsDown-RegulationEndotheliumImmunizationLymphangiogenesisLymphatic VesselsLymphotoxin beta ReceptorMiceMice, Inbred C57BLMice, KnockoutMucoproteinsOxazolonePhenotypeReceptors, Tumor Necrosis FactorTime FactorsT-LymphocytesVenulesConceptsHigh endothelial venulesB cellsEndothelial venulesPLN high endothelial venulesPeripheral lymph node high endothelial venulesLymph node high endothelial venulesMature phenotypeLTbetaR-Ig treatmentT cell primingLYVE-1Evans blue dyeLymphotoxin beta receptorLTbetaR-IgCell primingLV functionOVA immunizationImmature phenotypeDay 7Day 4Functional insufficiencyImmunizationClose physical contactLVRemodeling processLymphatic vessels
2005
Lymphotoxin Plays a Crucial Role in the Development and Function of Nasal-Associated Lymphoid Tissue through Regulation of Chemokines and Peripheral Node Addressin
Ying X, Chan K, Shenoy P, Hill M, Ruddle NH. Lymphotoxin Plays a Crucial Role in the Development and Function of Nasal-Associated Lymphoid Tissue through Regulation of Chemokines and Peripheral Node Addressin. American Journal Of Pathology 2005, 166: 135-146. PMID: 15632007, PMCID: PMC1602284, DOI: 10.1016/s0002-9440(10)62239-0.Peer-Reviewed Original ResearchConceptsHigh endothelial venulesLymphoid chemokinesIntranasal immunizationNasal-Associated Lymphoid TissueB cell compartmentalizationB cell zonesCervical lymph nodesSerum IgG titersLower cytokine levelsExpression of lymphotoxinImmediate postnatal periodRole of cytokinesRegulation of chemokinesWild-type miceGlyCAM-1Peripheral node addressinLymphoid tissue developmentNALT developmentSplenic cytokinesVaginal IgACytokine levelsLymph nodesIgG titersVascular addressinsLymphoid tissue
2002
Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice
Graesser D, Solowiej A, Bruckner M, Osterweil E, Juedes A, Davis S, Ruddle NH, Engelhardt B, Madri JA. Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice. Journal Of Clinical Investigation 2002, 109: 383-392. PMID: 11827998, PMCID: PMC150854, DOI: 10.1172/jci13595.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisPECAM-1-deficient miceEndothelial cellsAutoimmune encephalomyelitisVascular permeabilityDevelopment of EAET lymphocyte transendothelial migrationEarly onsetHuman autoimmune disease multiple sclerosisAutoimmune disease multiple sclerosisCell adhesion molecule-1Altered vascular permeabilityCNS vascular permeabilityMononuclear cell extravasationDisease multiple sclerosisPlatelet/endothelial cell adhesion molecule-1Wild-type miceAdhesion molecule-1Endothelial cell adhesion molecule-1Subsets of leukocytesPECAM-1 expressionLymphocyte transendothelial migrationEarly time pointsHistamine challengeMultiple sclerosis
2001
Resident and Infiltrating Central Nervous System APCs Regulate the Emergence and Resolution of Experimental Autoimmune Encephalomyelitis
Juedes A, Ruddle N. Resident and Infiltrating Central Nervous System APCs Regulate the Emergence and Resolution of Experimental Autoimmune Encephalomyelitis. The Journal Of Immunology 2001, 166: 5168-5175. PMID: 11290800, DOI: 10.4049/jimmunol.166.8.5168.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigen PresentationAntigen-Presenting CellsB7-1 AntigenBrainCell LineCell MovementDown-RegulationEncephalomyelitis, Autoimmune, ExperimentalFemaleGrowth InhibitorsHistocompatibility Antigens Class IHistocompatibility Antigens Class IILymphocyte ActivationMacromolecular SubstancesMacrophagesMiceMice, Inbred C57BLMice, KnockoutMicrogliaMolecular Sequence DataMyelin ProteinsMyelin-Associated GlycoproteinMyelin-Oligodendrocyte GlycoproteinNitric OxideSpinal CordT-LymphocytesConceptsExperimental autoimmune encephalomyelitisT cell proliferationT cellsT cell linesCNS APCCNS APCsAutoimmune encephalomyelitisMHC-IITarget organsInducible NO synthase-deficient miceT cell cytokine productionTh1 T cellsAutoreactive T cellsCell cytokine productionIFN-gamma productionB7-1 expressionSynthase-deficient miceCell proliferationMac-1 cellsCell linesInitiation of diseaseProduction of NOResident microgliaMyelin oligodendrocyteCytokine productionICOS co-stimulatory receptor is essential for T-cell activation and function
Dong C, Juedes A, Temann U, Shresta S, Allison J, Ruddle N, Flavell R. ICOS co-stimulatory receptor is essential for T-cell activation and function. Nature 2001, 409: 97-101. PMID: 11343121, DOI: 10.1038/35051100.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibody FormationAntigens, CDAntigens, Differentiation, T-LymphocyteCell DifferentiationCells, CulturedEncephalomyelitis, Autoimmune, ExperimentalGene TargetingHemocyaninsInducible T-Cell Co-Stimulator ProteinInterleukin-13Interleukin-4Lymph NodesLymphocyte ActivationMiceMice, KnockoutMolecular Sequence DataMyelin ProteinsMyelin-Associated GlycoproteinMyelin-Oligodendrocyte GlycoproteinT-LymphocytesConceptsInducible co-stimulatory moleculeT cell activationCo-stimulatory moleculesT cellsICOS-/- miceICOS-deficient miceInflammatory autoimmune diseaseExperimental autoimmune encephalomyelitisInjection of lipopolysaccharideCo-stimulatory receptorsHumoral immune responseNon-immune tissuesT lymphocyte activationAutoimmune encephalomyelitisAutoimmune diseasesImmune responseInterleukin-4Immune functionCD28/CTLA4 familyB cellsProtective roleEnhanced susceptibilityActivationReceptorsHigh affinity
1997
Distinct Roles in Lymphoid Organogenesis for Lymphotoxins α and β Revealed in Lymphotoxin β–Deficient Mice
Koni P, Sacca R, Lawton P, Browning J, Ruddle N, Flavell R. Distinct Roles in Lymphoid Organogenesis for Lymphotoxins α and β Revealed in Lymphotoxin β–Deficient Mice. Immunity 1997, 6: 491-500. PMID: 9133428, DOI: 10.1016/s1074-7613(00)80292-7.Peer-Reviewed Original ResearchConceptsMesenteric lymph nodesLT alpha-deficient miceAlpha-deficient miceFollicular dendritic cellsBeta-deficient miceLymph nodesDendritic cellsDeficient miceLymphoid organogenesisTumor necrosis factor receptor type ILTbeta-deficient micePeripheral lymph nodesReceptor type ISplenic germinal centersLymphotoxin βPeyer's patchesGerminal centersLymphotoxin alphaLT-alphaLT alpha3Lymphotoxin αMiceUnidentified receptorType IAlpha
1994
Abnormal Development of Peripheral Lymphoid Organs in Mice Deficient in Lymphotoxin
De Togni P, Goellner J, Ruddle N, Streeter P, Fick A, Mariathasan S, Smith S, Carlson R, Shornick L, Strauss-Schoenberger J, Russell J, Karr R, Chaplin D. Abnormal Development of Peripheral Lymphoid Organs in Mice Deficient in Lymphotoxin. Science 1994, 264: 703-707. PMID: 8171322, DOI: 10.1126/science.8171322.Peer-Reviewed Original ResearchConceptsPeripheral lymphoid organsPeripheral bloodLymphoid organsT cellsDetectable lymph nodesT cell subsetsLymph nodesCD4-CD8Cell subsetsPeyer's patchesMice DeficientImmunoglobulin MWhite pulpLymphotoxinLytic functionNormal ratioAbnormal developmentSpleenStem cellsBloodNormal developmentOrgansCellsLymphocytesThymus
1993
The lymphotoxin promoter is stimulated by HTLV-I tax activation of NF-κB in human T-cell lines
Paul N, Millet I, Ruddle N. The lymphotoxin promoter is stimulated by HTLV-I tax activation of NF-κB in human T-cell lines. Cytokine 1993, 5: 372-378. PMID: 7505113, DOI: 10.1016/1043-4666(93)90070-l.Peer-Reviewed Original Research1-Methyl-3-isobutylxanthineBase SequenceBinding SitesCell LineCell NucleusChloramphenicol O-AcetyltransferaseColforsinCyclic AMPCytotoxicity, ImmunologicGene Products, taxHuman T-lymphotropic virus 1HumansLymphotoxin-alphaMolecular Sequence DataNF-kappa BOligodeoxyribonucleotidesPromoter Regions, GeneticRepetitive Sequences, Nucleic AcidT-LymphocytesTransfectionTumor Cells, Cultured
1986
Isolation of antigen-binding membrane molecules from an antigen-specific T-cell hybrid
Cone R, Beaman K, Ruddle N. Isolation of antigen-binding membrane molecules from an antigen-specific T-cell hybrid. Cellular Immunology 1986, 99: 257-264. PMID: 3489563, DOI: 10.1016/0008-8749(86)90233-9.Peer-Reviewed Original Research
1982
A critical analysis of the T cell hybrid technique
Beezley B, Ruddle N. A critical analysis of the T cell hybrid technique. Journal Of Immunological Methods 1982, 52: 269-281. PMID: 6752275, DOI: 10.1016/0022-1759(82)90001-1.Peer-Reviewed Original Research
1981
T Cell Tumors, Clones, and Hybrids
Ruddle N. T Cell Tumors, Clones, and Hybrids. Chemical Immunology And Allergy 1981, 29: 222-268. PMID: 7036181, DOI: 10.1159/000400099.Peer-Reviewed Original Research
1980
Antigen-specific T-cell hybrids I. Ovalbumin binding T-cell hybrid
Whitaker R, Ruddle N. Antigen-specific T-cell hybrids I. Ovalbumin binding T-cell hybrid. Cellular Immunology 1980, 55: 56-65. PMID: 6159106, DOI: 10.1016/0008-8749(80)90136-7.Peer-Reviewed Original ResearchRegulation of self-recognition by T-cell hybrids
Ruddle N, Beezley B, Eardley D. Regulation of self-recognition by T-cell hybrids. Cellular Immunology 1980, 55: 42-55. PMID: 6968629, DOI: 10.1016/0008-8749(80)90135-5.Peer-Reviewed Original ResearchAntigen specific T cell hybrids—II T cell hybrids which bind azobenzenearsonate
Ruddle N, Beezley B, Lewis G, Goodman J. Antigen specific T cell hybrids—II T cell hybrids which bind azobenzenearsonate. Molecular Immunology 1980, 17: 925-931. PMID: 6163974, DOI: 10.1016/0161-5890(80)90041-3.Peer-Reviewed Original ResearchAntigen-specific molecules from murine T lymphocytes and T cell hybridomas
Goodman J, Lewis G, Primi D, Hornbeck P, Ruddle N. Antigen-specific molecules from murine T lymphocytes and T cell hybridomas. Molecular Immunology 1980, 17: 933-945. PMID: 6163975, DOI: 10.1016/0161-5890(80)90042-5.Peer-Reviewed Original ResearchConceptsJ miceT cellsABA-specific T cellsAntigen-specific suppressor activitySuppressor T cell responseSuppressor T cellsT cell responsesAntigen-specific moleculesLight chain determinantsABA-protein conjugatesT cell moleculesT cell hybridomasIa markersMurine T lymphocytesT lymphocytesRosette formationCell responsesMajor idiotypeSerological analysisCell hybridomasBW 5147Suppressor activityCell lysatesCell moleculesHybridoma cell lines