Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients
Qu R, Gupta K, Dong D, Jiang Y, Landa B, Saez C, Strickland G, Levinsohn J, Weng PL, Taketo MM, Kluger Y, Myung P. Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients. Developmental Cell 2022, 57: 1053-1067.e5. PMID: 35421372, PMCID: PMC9050909, DOI: 10.1016/j.devcel.2022.03.011.Peer-Reviewed Original ResearchConceptsMorphogen gradientsCell fate specificationWnt/β-cateninFate specificationShape tissuesOrgan formationCell fateDermal condensatesGenetic perturbationsNiche formationDifferentiation processSpatiotemporal patterningCell behaviorΒ-cateninMore intermediatesComputational approachProliferationMorphogenesisScRNAOrganogenesisShhKey componentProgenitorsCritical transitionDKK1Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis
Gupta K, Levinsohn J, Linderman G, Chen D, Sun TY, Dong D, Taketo MM, Bosenberg M, Kluger Y, Choate K, Myung P. Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis. Developmental Cell 2018, 48: 17-31.e6. PMID: 30595533, PMCID: PMC6361530, DOI: 10.1016/j.devcel.2018.11.032.Peer-Reviewed Original ResearchConceptsDermal condensatesSingle-cell RNA sequencingUnbiased single-cell RNA sequencingWnt/β-catenin signalingΒ-catenin signalingTranscriptional statesDifferentiation trajectoriesAppendage morphogenesisRNA sequencingHF morphogenesisCellular eventsRecent progenyCell differentiationMorphogenesisClusters of cellsMolecular differencesDistinctive populationCell analysisCellsDifferentiationHF developmentProgenySignalingDC cellsSequencing