2022
ÎČ Cellâspecific deletion of Zfp148 improves nutrient-stimulated ÎČ cell Ca2+ responses
Emfinger CH, de Klerk E, Schueler KL, Rabaglia ME, Stapleton DS, Simonett SP, Mitok KA, Wang Z, Liu X, Paulo JA, Yu Q, Cardone RL, Foster HR, Lewandowski SL, Perales JC, Kendziorski CM, Gygi SP, Kibbey RG, Keller MP, Hebrok M, Merrins MJ, Attie AD. ÎČ Cellâspecific deletion of Zfp148 improves nutrient-stimulated ÎČ cell Ca2+ responses. JCI Insight 2022, 7: e154198. PMID: 35603790, PMCID: PMC9220824, DOI: 10.1172/jci.insight.154198.Peer-Reviewed Original ResearchConceptsCell-specific deletionÎ-cell Ca2Insulin secretionAmino acid metabolismLow glucose conditionsRNA-seqPancreatic ÎČ-cellsLevels of enzymesZfp148Glutamate dehydrogenaseIntermediary metabolismChannel closureEnhanced insulin secretionWestern-style dietControl mice fedElevated glucose levelsAcid metabolismÎ-cellsCell functionGlucose toleranceCell Ca2Elevated sensitivityGlucose conditionsMetabolic challengesMice fed
2020
Pyruvate Kinase Controls Signal Strength in the Insulin Secretory Pathway
Lewandowski SL, Cardone RL, Foster HR, Ho T, Potapenko E, Poudel C, VanDeusen HR, Sdao SM, Alves TC, Zhao X, Capozzi ME, de Souza AH, Jahan I, Thomas CJ, Nunemaker CS, Davis DB, Campbell JE, Kibbey RG, Merrins MJ. Pyruvate Kinase Controls Signal Strength in the Insulin Secretory Pathway. Cell Metabolism 2020, 32: 736-750.e5. PMID: 33147484, PMCID: PMC7685238, DOI: 10.1016/j.cmet.2020.10.007.Peer-Reviewed Original ResearchConceptsPyruvate kinaseATP/ADPÎ-cell metabolismAppropriate insulin secretionPotential therapeutic routeSecretory pathwayMitochondrial fuelsPancreatic ÎČ-cellsInsulin secretory pathwayOxidative phosphorylationCell metabolismNutrient metabolismPhosphoenolpyruvateCell sensingPK activatorÎ-cellsCell functionInsulin secretionPK activityOxidative functionMembrane depolarizationMitochondriaPK activationÎ-cell functionADPGlucose Response by Stem Cell-Derived ÎČ Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis
Davis JC, Alves TC, Helman A, Chen JC, Kenty JH, Cardone RL, Liu DR, Kibbey RG, Melton DA. Glucose Response by Stem Cell-Derived ÎČ Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis. Cell Reports 2020, 31: 107623. PMID: 32402282, PMCID: PMC7433758, DOI: 10.1016/j.celrep.2020.107623.Peer-Reviewed Original ResearchConceptsSC-ÎČ cellsÎ-cellsHuman isletsReduced glucose-stimulated insulin secretionGlucose-stimulated insulin secretionStem cell-derived ÎČ cellsHuman ÎČ-cellsEnzyme glyceraldehydeTCA cycleGlucose challengeInsulin secretionInsulin releaseDiabetes treatmentGlucose responseCadaveric isletsBottleneck resultsEarly glycolysisTransplantable isletsCell functionIsletsIntermediate metabolitesConsistency of responsesGlycolysisCellsKinase