2024
β‑Amino Acids Reduce Ternary Complex Stability and Alter the Translation Elongation Mechanism
Cruz-Navarrete F, Griffin W, Chan Y, Martin M, Alejo J, Brady R, Natchiar S, Knudson I, Altman R, Schepartz A, Miller S, Blanchard S. β‑Amino Acids Reduce Ternary Complex Stability and Alter the Translation Elongation Mechanism. ACS Central Science 2024, 10: 1262-1275. PMID: 38947208, PMCID: PMC11212133, DOI: 10.1021/acscentsci.4c00314.Peer-Reviewed Original ResearchNon-natural amino acidsComplex stabilityTernary complex stabilityTemplate synthesisDetection limitEnergy transfer measurementsTernary complex formationComplex formationSingle-molecule fluorescence resonance energy transfer measurementsIn vitro detection limitTernary complexFluorescence resonance energy transfer measurementsAmino acidsMechanism of protein synthesisResonance energy transfer measurementsSynthesis of proteinsRate of translocationMRNA decodingElongation substratesStabilityElongation factorIsomersRibosome utilizationAminoacyl-tRNAStereoisomers
2017
Identification and Partial Structural Characterization of Mass Isolated Valsartan and Its Metabolite with Messenger Tagging Vibrational Spectroscopy
Gorlova O, Colvin SM, Brathwaite A, Menges FS, Craig SM, Miller SJ, Johnson MA. Identification and Partial Structural Characterization of Mass Isolated Valsartan and Its Metabolite with Messenger Tagging Vibrational Spectroscopy. Journal Of The American Society For Mass Spectrometry 2017, 28: 2414-2422. PMID: 28801884, DOI: 10.1007/s13361-017-1767-z.Peer-Reviewed Original ResearchVibrational spectroscopyStructural characterizationRole of conformersOH stretching fundamentalsPartial structural characterizationStructural isomersIsomeric formsTarget ionsMass spectrometryN2 moleculesDrug valsartanRf ion trapIR absorptionIon trapFingerprint regionK ionsIon approachSpectroscopyIonsIsomersCharacterizationConformersRecent advancesSpectrometryOH
2015
Enantioselective Synthesis of 3‑Arylquinazolin-4(3H)‑ones via Peptide-Catalyzed Atroposelective Bromination
Diener ME, Metrano AJ, Kusano S, Miller SJ. Enantioselective Synthesis of 3‑Arylquinazolin-4(3H)‑ones via Peptide-Catalyzed Atroposelective Bromination. Journal Of The American Chemical Society 2015, 137: 12369-12377. PMID: 26343278, PMCID: PMC5134330, DOI: 10.1021/jacs.5b07726.Peer-Reviewed Original ResearchConceptsAtroposelective brominationDensity functional theory calculationsBroad substrate scopeX-ray crystallographyΒ-turn peptideFunctional theory calculationsCross-coupling sequenceFree catalystsSubstrate scopeAmination procedureStereochemical informationEnantioselective synthesisTheory calculationsHigh enantioselectivityRotational barriersMechanistic studiesSubsequent transformationBrominationMono-orthoProduct of interestCatalystEnantioinductionCrystallographyEnantioselectivityIsomers