2015
Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2
Pimienta G, Fok V, Haslip M, Nagy M, Takyar S, Steitz JA. Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2. PLOS ONE 2015, 10: e0124638. PMID: 26121143, PMCID: PMC4487896, DOI: 10.1371/journal.pone.0124638.Peer-Reviewed Original ResearchConceptsMRNA-seq dataHost cell nucleusBJAB cellsCell proliferationGene expression featuresPro-survival effectsProtein adaptersAlternative splicingMRNA transcriptomeUpregulated proteinsSILAC dataRich elementsAkt activationPI3K-AktBiochemical assaysCell nucleiEBV latencySwitch eventsProteinMaintenance of latencyCell linesVEGFA proteinMechanistic explanationUpregulated oncogenesPIK3AP1
2005
mRNA Helicase Activity of the Ribosome
Takyar S, Hickerson RP, Noller HF. mRNA Helicase Activity of the Ribosome. Cell 2005, 120: 49-58. PMID: 15652481, DOI: 10.1016/j.cell.2004.11.042.Peer-Reviewed Original ResearchConceptsHelicase activitySecondary structureHelicase active siteMutation of residuesVitro translation systemMRNA secondary structureDownstream mRNA secondary structureAbility of ribosomesDownstream helicesProtein S3Most mRNAsBase-paired helicesRibosomesTranslation systemHelixActive siteDisplacement assayDownstream tunnelHelicaseCodonProcessivitySubunitsMutationsMRNAResidues
2002
Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site
Takyar S, Gowans E, Lott W. Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site. Journal Of Molecular Biology 2002, 319: 1-8. PMID: 12051931, DOI: 10.1016/s0022-2836(02)00300-5.Peer-Reviewed Original ResearchConceptsIRES-dependent translationRibosome entry siteHepatitis C virus internal ribosome entry siteInternal ribosome entry siteEntry siteTranslation inhibitor cycloheximideS ribosomal complexCap-dependent mRNAMRNA-specificGradient centrifugation analysisSucrose gradient centrifugation analysisCompetitive binding mechanismHCV IRESRibosomal complexesTranslation inhibitorsInhibitor cycloheximideCentrifugation analysisMRNAComplex formationCycloheximideBinding mechanism