2005
mRNA Helicase Activity of the Ribosome
Takyar S, Hickerson RP, Noller HF. mRNA Helicase Activity of the Ribosome. Cell 2005, 120: 49-58. PMID: 15652481, DOI: 10.1016/j.cell.2004.11.042.Peer-Reviewed Original ResearchConceptsHelicase activitySecondary structureHelicase active siteMutation of residuesVitro translation systemMRNA secondary structureDownstream mRNA secondary structureAbility of ribosomesDownstream helicesProtein S3Most mRNAsBase-paired helicesRibosomesTranslation systemHelixActive siteDisplacement assayDownstream tunnelHelicaseCodonProcessivitySubunitsMutationsMRNAResidues
2002
Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site
Takyar S, Gowans E, Lott W. Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site. Journal Of Molecular Biology 2002, 319: 1-8. PMID: 12051931, DOI: 10.1016/s0022-2836(02)00300-5.Peer-Reviewed Original ResearchConceptsIRES-dependent translationRibosome entry siteHepatitis C virus internal ribosome entry siteInternal ribosome entry siteEntry siteTranslation inhibitor cycloheximideS ribosomal complexCap-dependent mRNAMRNA-specificGradient centrifugation analysisSucrose gradient centrifugation analysisCompetitive binding mechanismHCV IRESRibosomal complexesTranslation inhibitorsInhibitor cycloheximideCentrifugation analysisMRNAComplex formationCycloheximideBinding mechanism