NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome
Fang EF, Hou Y, Lautrup S, Jensen MB, Yang B, SenGupta T, Caponio D, Khezri R, Demarest TG, Aman Y, Figueroa D, Morevati M, Lee HJ, Kato H, Kassahun H, Lee JH, Filippelli D, Okur MN, Mangerich A, Croteau DL, Maezawa Y, Lyssiotis CA, Tao J, Yokote K, Rusten TE, Mattson MP, Jasper H, Nilsen H, Bohr VA. NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome. Nature Communications 2019, 10: 5284. PMID: 31754102, PMCID: PMC6872719, DOI: 10.1038/s41467-019-13172-8.Peer-Reviewed Original ResearchMeSH KeywordsAging, PrematureAnimalsAutophagy-Related Protein-1 HomologCaenorhabditis elegansCation Transport ProteinsDisease Models, AnimalDrosophila melanogasterHumansIntracellular Signaling Peptides and ProteinsMitophagyMutationNADNicotinamide-Nucleotide AdenylyltransferaseWerner SyndromeWerner Syndrome HelicaseConceptsWerner syndromeWerner DNA helicasePremature aging diseaseDrosophila melanogaster modelStem cell dysfunctionCaenorhabditis elegansDNA helicaseOrganismal levelImpaired mitochondrial functionMitochondrial qualityWS phenotypeImpaired mitophagyMitophagyMitochondrial functionDCT-1Ubiquitous moleculeSevere metabolic phenotypeMetabolic phenotypePhenotypeW patientsMetabolic dysfunctionCell dysfunctionMetabolic deficitsTherapeutic interventionsUnderlying mechanismHydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells
Libiad M, Vitvitsky V, Bostelaar T, Bak DW, Lee HJ, Sakamoto N, Fearon E, Lyssiotis CA, Weerapana E, Banerjee R. Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells. Journal Of Biological Chemistry 2019, 294: 12077-12090. PMID: 31213529, PMCID: PMC6690701, DOI: 10.1074/jbc.ra119.009442.Peer-Reviewed Original ResearchConceptsSulfide quinone oxidoreductaseSulfide oxidation pathwayElectron transfer chainColon epithelial cellsMetabolic reprogrammingGlutamine-dependent reductive carboxylationMitochondrial bioenergeticsMitochondrial sulfide oxidation pathwayCentral carbon metabolismEpithelial cellsHost-microbiome interfaceS oxidation pathwayCarbon metabolismHuman colonic cryptsOxidation pathwayPathway enzymesCellular bioenergeticsGut microbial metabolismStress responseMicrobial metabolismTransfer chainReductive carboxylationReductive shiftOxygen consumption rateQuinone oxidoreductase