Featured Publications
A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics
Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Nature Methods 2022, 19: 1371-1375. PMID: 36280721, PMCID: PMC9847208, DOI: 10.1038/s41592-022-01638-5.Peer-Reviewed Original ResearchConceptsPhosphorylation site localizationPhosphorylation positionsCellular signalingPosition of phosphorylationProtein phosphorylationComplex biological systemsPhosphorylated isomersData analysis pipelinePhosphoproteomicsAnalysis pipelinePhosphopeptide standardsPhosphorylationPhosphopeptidesCentral roleBiological systemsWeb toolLocalizationSignalingFragmentation spectra
2023
System‐wide optimization of an orthogonal translation system with enhanced biological tolerance
Mohler K, Moen J, Rogulina S, Rinehart J. System‐wide optimization of an orthogonal translation system with enhanced biological tolerance. Molecular Systems Biology 2023, 19: msb202110591. PMID: 37477096, PMCID: PMC10407733, DOI: 10.15252/msb.202110591.Peer-Reviewed Original ResearchConceptsOrthogonal translation systemHost interactionsNon-standard amino acidsPost-translational modificationsSystems-level biologyStress response activationTranslation systemSynthetic biological systemsCellular physiologyProtein phosphorylationOTS performanceHost physiologyCellular environmentAmino acidsCellular mechanismsDeleterious interactionsResponse activationBiological systemsPhysiologyOTS developmentUnparalleled accessPhosphorylationHost toxicityBiologyInteraction