Featured Publications
Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function
Schiapparelli P, Pirman NL, Mohler K, Miranda-Herrera PA, Zarco N, Kilic O, Miller C, Shah SR, Rogulina S, Hungerford W, Abriola L, Hoyer D, Turk BE, Guerrero-Cázares H, Isaacs FJ, Quiñones-Hinojosa A, Levchenko A, Rinehart J. Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function. Cell Reports 2021, 36: 109416. PMID: 34289367, PMCID: PMC8379681, DOI: 10.1016/j.celrep.2021.109416.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell Line, TumorCell MovementCell ProliferationEscherichia coliFemaleGlioblastomaHEK293 CellsHumansMaleMice, NudeMiddle AgedPhosphorylationPhosphoserineProtein Serine-Threonine KinasesRecombinant ProteinsSignal TransductionSmall Molecule LibrariesSubstrate SpecificityWNK Lysine-Deficient Protein Kinase 1ConceptsKinase networkAuthentic post-translational modificationsGenetic code expansionPost-translational modificationsProduction of proteinsSmall molecule kinase inhibitorsKinase inhibitorsGenetic codePhosphorylated proteinsCode expansionKinase proteinWNK kinasesPhysiological functionsWNK4 kinaseBiochemical propertiesGlioblastoma cellsKinaseBacterial strainsProteinDistinct sitesPhosphoserineSPAKBacteriaCellular systemsCells
2013
Src‐family protein tyrosine kinase (SFK) stimulates KCNJ10 K channels in the basolateral membrane of distal convoluted tubules (DCT).
Wang W, Zhang C, Lin D, Yue P, Wang L, Rinehart J. Src‐family protein tyrosine kinase (SFK) stimulates KCNJ10 K channels in the basolateral membrane of distal convoluted tubules (DCT). The FASEB Journal 2013, 27: 911.1-911.1. DOI: 10.1096/fasebj.27.1_supplement.911.1.Peer-Reviewed Original ResearchSrc family protein tyrosine kinasesPhosphorylation sitesWhole-cell K currentsTyrosine phosphorylationTyr-8Tyr-9Tyrosine phosphorylation sitesProtein tyrosine kinasesTyrosine phosphorylated proteinsBasolateral K channelsPhosphorylated proteinsBasolateral Cl(-) exitSFK activityHigh K dietTyrosine kinaseK channelsPhysiological roleHEK293 cellsCl exitHEK cellsDCT1Patch-clamp experimentsBasolateral membraneKCNJ10Phosphorylation