The U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome
Sontheimer E, Steitz J. The U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome. Science 1993, 262: 1989-1996. PMID: 8266094, DOI: 10.1126/science.8266094.Peer-Reviewed Original ResearchConceptsSmall nuclear RNANuclear RNAPrecursor messenger RNA splicingLariat intermediateU6 small nuclear RNAMessenger RNA splicingExon 1Self-splicing intronsActive site componentsRNA splicingMammalian spliceosomeU6 RNARNA contactsMechanistic parallelsPre-mRNAVitro splicingSubsequent splicingSplicingSpliceosomeSplice siteLast residueFirst residueFunctional interactionIntron productsRNAMutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo.
Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo. The EMBO Journal 1993, 12: 5181-5189. PMID: 8262061, PMCID: PMC413781, DOI: 10.1002/j.1460-2075.1993.tb06213.x.Peer-Reviewed Original ResearchConceptsSplice siteRabbit beta-globin geneBeta-globin transcriptsBeta-globin geneU5 genesConserved loopMRNA splicingU5 snRNASecond intronFirst exonExon sequencesLoop mutantsSecond exonExpression vectorTransient transfectionCryptic sitesNovel siteHeLa cellsGT dinucleotideExonsExon 1U5 sequencesSnRNAMutationsVivo system