2017
Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats
Kitay AM, Link A, Geibel JP. Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats. Cellular Physiology And Biochemistry 2017, 44: 1606-1615. PMID: 29212068, DOI: 10.1159/000485755.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseGastric acid secretionAcid secretionL-arginineL-NAMENO/sGC/cGMP pathwayNitric oxideSGC/cGMP pathwayParietal cellsNitric oxide synthase stimulationSGC inhibitor ODQL-arginine exposureAcid-related diseasesPossible intracellular mechanismsNovel secretagogueRat parietal cellsIsolated gastric glandsUlcer diseaseOxide synthaseCGMP pathwayImmune functionImportant mediatorIntracellular mechanismsSecretionGastric glandsAcute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation
Aldajani MM, Vanicek CN, Alhazzaa N, Lysyy T, Agarwal R, Geibel JP. Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation. Cellular Physiology And Biochemistry 2017, 44: 377-387. PMID: 29132138, DOI: 10.1159/000484914.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAscorbic AcidCell MembraneColonHydrogen-Ion ConcentrationIn Vitro TechniquesIntestinal MucosaMaleRatsRats, Sprague-DawleySodiumSodium-Coupled Vitamin C TransportersConceptsVitamin CColonic cryptsAcute effectsDependent intracellular pH recoveryTumor formationIntracellular pH recoveryNew therapeutic agentsMM vitamin CColorectal cancerDistal colonLeading causeTranscellular passive diffusionBasolateral membraneAcute additionNormal colonic cryptsC exposureNutraceutical agentTherapeutic agentsPH recoveryIntracellular pH changesCancer cellsVitamin C.CryptsLow intracellularTight junctions
2013
Effect of NSAIDs on Na+/H+ exchanger activity in rat colonic crypts
Roginiel AC, Kohut DL, Kaur S, Saleh AM, Weber T, Geibel P, Singh H, Geibel JP. Effect of NSAIDs on Na+/H+ exchanger activity in rat colonic crypts. American Journal Of Physiology - Cell Physiology 2013, 305: c512-c518. PMID: 23739181, DOI: 10.1152/ajpcell.00303.2012.Peer-Reviewed Original ResearchConceptsRat colonic cryptsExchanger activityNonsteroidal anti-inflammatory drugsUpper GI injuryColonic cryptsLower GI bleedingUse of NSAIDsChronic inflammatory stateLower GI tractUse of suppositoriesEffects of NSAIDsAnti-inflammatory drugsGI injuryNSAIDs aspirinGI bleedingPain reliefRheumatoid diseaseEpithelial erosionsInflammatory stateMyocardial infarctionChronic conditionsMucosal barrierUlcer formationColonic tissueGastrointestinal tractThe calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule
Capasso G, Geibel PJ, Damiano S, Jaeger P, Richards WG, Geibel JP. The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule. Kidney International 2013, 84: 277-284. PMID: 23615500, DOI: 10.1038/ki.2013.137.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsCalcimimetic AgentsCalciumHydrogen-Ion ConcentrationIn Vitro TechniquesKidney Tubules, ProximalMaleMiceMice, KnockoutNuclear ProteinsPerfusionPuncturesRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, G-Protein-CoupledSodium-Hydrogen ExchangersTranscription FactorsConceptsProximal tubulesLuminal calcium concentrationFluid reabsorptionAcid secretionKnockout miceCalcium ion concentrationCalcium concentrationRole of CaSRActivation of CaSRCalcium sensing receptorCalcium-sensing receptorMouse proximal tubulesProton secretionSodium-hydrogen exchangerCalcimimetic agentBicarbonate absorptionSensing receptorCaSRFluid absorptionSecretionReabsorptionMiceCalcimimeticsTubulesLuminal membraneGastric Acid, Calcium Absorption, and Their Impact on Bone Health
Kopic S, Geibel JP. Gastric Acid, Calcium Absorption, and Their Impact on Bone Health. Physiological Reviews 2013, 93: 189-268. PMID: 23303909, DOI: 10.1152/physrev.00015.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntacidsBone and BonesBone Diseases, MetabolicCalcitoninCalciumGastric AcidGastric MucosaHistamine H2 AntagonistsHomeostasisHumansHydrogen-Ion ConcentrationIntestinal AbsorptionIntestinal MucosaIntestinesParathyroid HormoneProton Pump InhibitorsReceptors, Calcium-SensingSignal TransductionVitamin DConceptsBone healthCalcium absorptionIntestinal calcium absorptionGastric acid secretionSystemic calcium homeostasisAdequate intestinal absorptionGastric acidityCalcium balanceGastric acidAcid secretionIntestinal absorptionCalcium homeostasisNeuroendocrine regulationCalcium uptakePhysiological processesPhysiological basisPotential linkHealthSecretionIntestine
2011
Angiotensin II Stimulates H+-ATPase Activity in Intercalated Cells from Isolated Mouse Connecting Tubules and Cortical Collecting Ducts
Wagner CA, Mohebbi N, Uhlig U, Giebisch GH, Breton S, Brown D, Geibel JP. Angiotensin II Stimulates H+-ATPase Activity in Intercalated Cells from Isolated Mouse Connecting Tubules and Cortical Collecting Ducts. Cellular Physiology And Biochemistry 2011, 28: 513-520. PMID: 22116365, PMCID: PMC3709473, DOI: 10.1159/000335112.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsAnion Transport ProteinsBicarbonatesCell MembraneChloridesColchicineHydrogen-Ion ConcentrationImmunohistochemistryKidney CortexKidney Tubules, CollectingMacrolidesMaleMiceMice, Inbred C57BLReceptors, AngiotensinSaralasinSodiumSulfate TransportersVacuolar Proton-Translocating ATPasesConceptsCNT/CCDAngiotensin IICortical collecting ductsBicarbonate secretionChloride absorptionCollecting ductsType 1 angiotensin II receptorAngiotensin II stimulatesAngiotensin II receptorsAcid-base statusConnecting tubuleATPase activityII receptorsSpecific subtypesNephron segmentsIndependent intracellularAcid extrusionType BSecretionTubulesDuct systemDuctPendrinPresent studyCellsFunctional role of NHE4 as a pH regulator in rat and human colonic crypts
Arena EA, Longo WE, Roberts KE, Geibel P, Nateqi J, Brandstetter M, Geibel JP. Functional role of NHE4 as a pH regulator in rat and human colonic crypts. American Journal Of Physiology - Cell Physiology 2011, 302: c412-c418. PMID: 22049213, DOI: 10.1152/ajpcell.00163.2011.Peer-Reviewed Original ResearchConceptsHuman colonic cryptsNHE4 activityColonic cryptsElective colon resectionEffects of aldosteroneSprague-Dawley ratsColon resectionRat modelGastrointestinal tractAldosteroneColonic samplesΜM forskolinFluid homeostasisRatsHuman colonHuman specimensCAMP levelsIntracellular pathwaysCryptsNHE4ColonSignificant changesLittle descriptionFunctional roleRecent studiesThe flavonone naringenin inhibits chloride secretion in isolated colonic epithelia
Collins D, Kopic S, Geibel JP, Hogan AM, Medani M, Baird AW, Winter DC. The flavonone naringenin inhibits chloride secretion in isolated colonic epithelia. European Journal Of Pharmacology 2011, 668: 271-277. PMID: 21762688, DOI: 10.1016/j.ejphar.2011.06.052.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChloridesColonCyclic AMPElectric ConductivityEpitheliumFlavanonesHumansHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular SpaceMaleRatsRats, WistarConceptsHuman colonic mucosaChloride secretionAntisecretory actionColonic mucosaCo-transporter inhibitorsEffect of naringeninIntracellular cAMP generationColonic cell lineAntisecretory effectColonic functionColorectal cancerProtective effectUssing chambersColonic epitheliumSecretory functionCAMP generationInhibitory actionBasolateral NKCC1Human colonRatsSecretionIntracellular acidificationConflicting resultsCell linesMucosa
2010
Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion
Kirchhoff P, Socrates T, Sidani S, Duffy A, Breidthardt T, Grob C, Viehl CT, Beglinger C, Oertli D, Geibel JP. Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion. The American Journal Of Gastroenterology 2010, 106: 62. PMID: 20736941, DOI: 10.1038/ajg.2010.327.Peer-Reviewed Original ResearchConceptsProton pump inhibitorsGastric acid secretionAcid secretionRat gastric glandsRandomized cross-over studyIntact rat stomachAcid suppression therapyGastric glandsCross-over studyOnset of actionLong-term efficacyReturn of symptomsHealthy human volunteersSecretagogue-induced acid secretionStomach acid secretionProlonged therapySuppression therapyIntragastric pHPump inhibitorsSingle doseWhole stomachLifetime prevalenceRat modelRat stomachFaster onsetEthanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity
Kopic S, Corradini S, Sidani S, Murek M, Vardanyan A, Föller M, Ritter M, Geibel JP. Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity. Cellular Physiology And Biochemistry 2010, 25: 195-202. PMID: 20110680, DOI: 10.1159/000276553.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionSecretagogue-induced acid secretionInhibits gastric acid secretionShort-term ethanol exposurePH-sensitive dye BCECFLow-dose ethanolCompound CAMPK inhibitor compound CSpecific AMPK inhibitor compound CEffects of ethanolInhibitor compound CEthanol exposureRat gastric glandsActivation of AMPKInteraction of ethanolRat gastric parietal cellsFluorescence digital imagingGastric parietal cellsAMP kinase activityDye BCECFInhibitory effectSecretionParietal cellsAMPK pathway
2009
Revisiting the parietal cell
Kopic S, Murek M, Geibel JP. Revisiting the parietal cell. American Journal Of Physiology - Cell Physiology 2009, 298: c1-c10. PMID: 19889964, DOI: 10.1152/ajpcell.00478.2009.Peer-Reviewed Original ResearchAMP-activated protein kinase: a physiological off switch for murine gastric acid secretion
Sidani S, Kopic S, Socrates T, Kirchhoff P, Föller M, Murek M, Capasso A, Geibel JP. AMP-activated protein kinase: a physiological off switch for murine gastric acid secretion. Pflügers Archiv - European Journal Of Physiology 2009, 459: 39. PMID: 19621238, DOI: 10.1007/s00424-009-0698-3.Peer-Reviewed Original ResearchConceptsProtein kinaseAMPK activationSecretagogue-induced acid secretionCellular ATP levelsPresence of AMPKEnergy-dependent transportersImmunofluorescent localizationParietal cellsGastric glandsCellular ATP consumptionGastric parietal cellsCompound CAMPKProton effluxATP consumptionMetabolic stressRate of intracellularATP levelsKinaseAdenosine monophosphateRegulatorATPLarge abundanceCellsActivation
2007
Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells
Barmeyer C, Ye JH, Sidani S, Geibel J, Binder HJ, Rajendran VM. Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 441-450. PMID: 17492310, DOI: 10.1007/s00424-007-0213-7.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceBicarbonatesCell LineChloridesCloning, MolecularColonDNA PrimersFatty Acids, VolatileHumansHydrogen-Ion ConcentrationIon TransportMolecular Sequence DataRatsRecombinant ProteinsSequence Homology, Amino AcidSulfate TransportersTransfectionConceptsRat distal colonDistal colonHEK-293 cellsExtracellular Cl- concentrationCrypt cellsMRNA expressionColonRatsDigestive tractCharacteristics of ratsAdenomasHigh rateDIDSCl- influxSurface cellsIntracellular acidApical membrane vesiclesCellsDisulfonic acidApical membraneCl- uptakePrior observationsUptakeLungJejunum
2006
Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands
Kirchhoff P, Andersson K, Socrates T, Sidani S, Kosiek O, Geibel J. Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands. AJP Gastrointestinal And Liver Physiology 2006, 291: g838-g843. PMID: 16798725, DOI: 10.1152/ajpgi.00120.2006.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionRat gastric glandsPotassium-competitive acid blockerGastric glandsPeptic ulcer diseaseProton pump inhibitor omeprazoleAcid-related diseasesCompetitive acid blockerPresence of omeprazoleLow-dose inhibitorsFree perfusateUlcer diseasePharmacological treatmentAZD0865High success rateAcid blockerLow doseRapid onsetSensitive dye BCECFInhibitor omeprazoleAcid loadOmeprazoleDye BCECFSecretionDistal tubule acidification.
Geibel JP. Distal tubule acidification. Journal Of Nephrology 2006, 19 Suppl 9: s18-26. PMID: 16736436.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsHydrogen-Ion ConcentrationIon TransportKidney Tubules, DistalProton-Translocating ATPases
2005
An amino acid transporter involved in gastric acid secretion
Kirchhoff P, Dave MH, Remy C, Kosiek O, Busque SM, Dufner M, Geibel JP, Verrey F, Wagner CA. An amino acid transporter involved in gastric acid secretion. Pflügers Archiv - European Journal Of Physiology 2005, 451: 738-748. PMID: 16308696, DOI: 10.1007/s00424-005-1507-2.Peer-Reviewed Original ResearchConceptsAmino acid transportersAcid transportersAmino acidsHeteromeric amino acid transportersHeterodimeric amino acid transportersGastric acid secretionSystem L amino acid transportProtein-rich dietSystem L amino acid transportersSystem L aminoL amino acid transportersAmino acid transportParietal cellsAcid secretionPresence of glutamineNovel roleAcid transportVariety of stimuliIsolated rat gastric glandsTransportersATPase activityNon-metabolizable substratesSystem L.Serum amino acidsGlutamineThe B1-subunit of the H+ ATPase is required for maximal urinary acidification
Finberg KE, Wagner CA, Bailey MA, Păunescu T, Breton S, Brown D, Giebisch G, Geibel JP, Lifton RP. The B1-subunit of the H+ ATPase is required for maximal urinary acidification. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13616-13621. PMID: 16174750, PMCID: PMC1224669, DOI: 10.1073/pnas.0506769102.Peer-Reviewed Original ResearchConceptsMaximal urinary acidificationNormal urinary acidificationMetabolic acidosisUrinary acidificationDistal renal tubular acidosisAcid challengeRenal tubular acidosisAcute intracellular acidificationB1 subunitLumen-negative potentialFurosemide infusionTubular acidosisAlkaline urineDistal nephronGreater severityAcidosisMiceLoss of B1Intracellular acidificationApical expressionUrineDuctFurther decreaseIsoformsPlasma membraneRole of short-chain fatty acids in colonic HCO3 secretion
Vidyasagar S, Barmeyer C, Geibel J, Binder HJ, Rajendran VM. Role of short-chain fatty acids in colonic HCO3 secretion. AJP Gastrointestinal And Liver Physiology 2005, 288: g1217-g1226. PMID: 15677553, DOI: 10.1152/ajpgi.00415.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesButyric AcidChlorineColonCyclic AMPDown-RegulationFatty Acids, VolatileHydrogen-Ion ConcentrationIntestinal MucosaIon ChannelsMaleRatsRats, Sprague-DawleyConceptsShort-chain fatty acidsSCFA/SCFA transportApical membrane vesiclesMM butyrateSurface epithelial cellsFatty acidsSCFA metabolismColonic mucosaHCO3- secretionCrypt cellsCarrier-mediated processLucite chamberSecretionColonic cryptsEpithelial cellsComparable degreeButyrate stimulationChannel activityButyrate uptakeStat techniqueCryptsAnion channel activityDIDSButyrate
2004
Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium
Fidzinski P, Salvador-Silva M, Choritz L, Geibel J, Coca-Prados M. Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium. American Journal Of Physiology - Cell Physiology 2004, 287: c655-c663. PMID: 15140751, DOI: 10.1152/ajpcell.00552.2003.Peer-Reviewed Original ResearchConceptsC-type natriuretic peptideAtrial natriuretic peptideBrain natriuretic peptideNatriuretic peptideIntraocular pressureCiliary epitheliumNHE activityNatriuretic peptides atrial natriuretic peptideInhibitory effectType B receptorsNHE-1 activityNonpigmented ciliary epitheliumCell layerGap junction blockersDose-dependent mannerInhibitors of NHEOcular ciliary epitheliumRate of intracellularHypotensive effectJunction blockersTrabecular meshworkMammalian eyeExchanger activityB receptorNHE-1Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone
Winter C, Schulz N, Giebisch G, Geibel JP, Wagner CA. Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 2636-2641. PMID: 14983061, PMCID: PMC357002, DOI: 10.1073/pnas.0307321101.Peer-Reviewed Original ResearchConceptsAcid-base homeostasisMineralocorticoid receptorStimulatory effectAldosterone-induced stimulationSystemic acid-base homeostasisDistal tubular acidosisRenal tubule cellsAcid-base statusNongenomic modulationNongenomic stimulationRenal collecting ductsAngiotensin IITransepithelial pH gradientTubular acidosisUrine acidificationAldosteroneATPase activityRenal acidificationHydrogen secretionIntracellular Ca2Complete reabsorptionATPase stainingTubule cellsCollecting ductsNongenomic pathways