2019
Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification
Penny SA, Malaker SA. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification. Methods In Molecular Biology 2019, 2024: 235-243. PMID: 31364053, DOI: 10.1007/978-1-4939-9597-4_14.ChaptersConceptsMass spectrometryAntibody conjugationImmunoaffinity purificationSurface of cellsMajor histocompatibility complexAutoimmune diseasesT cellsImmune componentsDisease processMoleculesHistocompatibility complexAntigenic peptidesMHCAssociated peptidePurificationPeptidesSurfaceSpectrometryElutionCellsComplexesConjugationCD8CD4CancerMass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways
Malaker SA, Ferracane MJ. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways. Methods In Molecular Biology 2019, 2024: 269-285. PMID: 31364056, DOI: 10.1007/978-1-4939-9597-4_17.Chapters
2017
Shared peptide binding of HLA Class I and II alleles associate with cutaneous nevirapine hypersensitivity and identify novel risk alleles
Pavlos R, McKinnon EJ, Ostrov DA, Peters B, Buus S, Koelle D, Chopra A, Schutte R, Rive C, Redwood A, Restrepo S, Bracey A, Kaever T, Myers P, Speers E, Malaker SA, Shabanowitz J, Jing Y, Gaudieri S, Hunt DF, Carrington M, Haas DW, Mallal S, Phillips EJ. Shared peptide binding of HLA Class I and II alleles associate with cutaneous nevirapine hypersensitivity and identify novel risk alleles. Scientific Reports 2017, 7: 8653. PMID: 28819312, PMCID: PMC5561238, DOI: 10.1038/s41598-017-08876-0.Peer-Reviewed Original ResearchConceptsHypersensitivity reactionsHLA moleculesTreatment-limiting hypersensitivity reactionClass IDrug hypersensitivity syndromeHuman leukocyte antigen (HLA) systemHLA class IHLA-C allelesClass II allelesLeukocyte antigen systemHLA-B allelesNevirapine hypersensitivityHypersensitivity syndromeHLA associationsIndependent associationHLA-DRB1T cellsImmune responseNovel risk allelesPathogenic mechanismsDrug specific interactionsMultiple class IAntigen systemPrimary predispositionRisk alleles
2013
MHC Class I–Associated Phosphopeptides Are the Targets of Memory-like Immunity in Leukemia
Cobbold M, De La Peña H, Norris A, Polefrone JM, Qian J, English AM, Cummings KL, Penny S, Turner JE, Cottine J, Abelin JG, Malaker SA, Zarling AL, Huang HW, Goodyear O, Freeman SD, Shabanowitz J, Pratt G, Craddock C, Williams ME, Hunt DF, Engelhard VH. MHC Class I–Associated Phosphopeptides Are the Targets of Memory-like Immunity in Leukemia. Science Translational Medicine 2013, 5: 203ra125. PMID: 24048523, PMCID: PMC4071620, DOI: 10.1126/scitranslmed.3006061.Peer-Reviewed Original ResearchConceptsT cellsImmune surveillanceAllogeneic stem cell transplantationMajor histocompatibility complex class IStem cell transplantationT cell responsesHistocompatibility complex class IAdoptive transfer immunotherapyCancer immune surveillanceHuman leukocyte antigenComplex class ICell linesPrimary leukemia cellsT cell linesClinical outcomesCell transplantationLeukocyte antigenLeukemia patientsMemory compartmentHematological tumorsHealthy individualsLeukemia cell linesCell responsesMalignant transformationNormal tissues