2020
Cutting Edge: The Use of Topical Aminoglycosides as an Effective Pull in "Prime and Pull" Vaccine Strategy.
Gopinath S, Lu P, Iwasaki A. Cutting Edge: The Use of Topical Aminoglycosides as an Effective Pull in "Prime and Pull" Vaccine Strategy. The Journal Of Immunology 2020, 204: 1703-1707. PMID: 32122994, DOI: 10.4049/jimmunol.1900462.Peer-Reviewed Original ResearchConceptsTissue-resident memory T cellsGenital herpes infectionMemory T cellsT cellsHerpes infectionVirus-specific effector T cellsVaginal applicationTopical vaginal applicationCD8 T cellsEffector T cellsProtective immune responseSingle topical applicationTopical aminoglycosidesGenital mucosaChemokine expressionVaccine strategiesImmune responseVaginal mucosaTopical applicationBarrier tissuesMiceRobust activationAminoglycoside antibioticsMucosaInfectionChapter 43 Mucosal Vaccines for Genital Herpes
Oh J, Iwasaki A. Chapter 43 Mucosal Vaccines for Genital Herpes. 2020, 723-734. DOI: 10.1016/b978-0-12-811924-2.00043-2.Peer-Reviewed Original ResearchGenital herpesMucosal vaccinesHSV-2Immune responseSensory neuronsHerpes simplex virus type 2Simplex virus type 2Genital mucosal epitheliaLocal immune responseDorsal root gangliaHSV type 1Host immune responseVirus type 2Painful ulcersGenital mucosaMucosal immunityAsymptomatic sheddingHIV transmissionRoot gangliaRisk factorsSystemic immunityClinical trialsLifelong infectionMucosal epitheliumAntiviral drugs
2016
Vaginal Exposure to Zika Virus during Pregnancy Leads to Fetal Brain Infection
Yockey LJ, Varela L, Rakib T, Khoury-Hanold W, Fink SL, Stutz B, Szigeti-Buck K, Van den Pol A, Lindenbach BD, Horvath TL, Iwasaki A. Vaginal Exposure to Zika Virus during Pregnancy Leads to Fetal Brain Infection. Cell 2016, 166: 1247-1256.e4. PMID: 27565347, PMCID: PMC5006689, DOI: 10.1016/j.cell.2016.08.004.Peer-Reviewed Original ResearchMeSH KeywordsAbortion, HabitualAnimalsBrainBrain DiseasesDisease Models, AnimalFemaleFetal Growth RetardationInterferon Regulatory Factor-3MiceMice, Inbred C57BLMice, Mutant StrainsPregnancyPregnancy Complications, InfectiousReceptor, Interferon alpha-betaVaginaVirus ReplicationZika VirusZika Virus InfectionConceptsZika virusFetal brain infectionFetal growth restrictionLocal viral replicationWild-type miceType I interferon receptorZIKV challengeTranscription factor IRF3Vaginal exposureGenital mucosaBrain infectionWT miceEarly pregnancyZIKV infectionGrowth restrictionPregnant damsVaginal infectionsZIKV replicationFetal brainMouse modelIFN pathwayVaginal tractUnborn fetusViral replicationDisease consequences
2014
A local macrophage chemokine network sustains protective tissue-resident memory CD4 T cells
Iijima N, Iwasaki A. A local macrophage chemokine network sustains protective tissue-resident memory CD4 T cells. Science 2014, 346: 93-98. PMID: 25170048, PMCID: PMC4254703, DOI: 10.1126/science.1257530.Peer-Reviewed Original ResearchConceptsMemory lymphocyte clustersMemory T cellsT cellsCD8 tissue-resident memory T cellsTissue-resident memory CD4 T cellsHerpes simplex virus 2 infectionTissue-resident memory T cellsSimplex virus 2 infectionMemory CD4 T cellsEfficient local controlPool of CD4Role of CD4CD4 T cellsVirus 2 infectionVaginal CD4Parabiotic miceGenital mucosaVaccine strategiesChemokine networkLymphocyte clustersHSV-2Viral infectionCD4Local controlInfection
2010
Antiviral immune responses in the genital tract: clues for vaccines
Iwasaki A. Antiviral immune responses in the genital tract: clues for vaccines. Nature Reviews Immunology 2010, 10: 699-711. PMID: 20829886, PMCID: PMC3678359, DOI: 10.1038/nri2836.Peer-Reviewed Original ResearchConceptsGenital mucosaT cellsGenital tractHerpes simplex virus type 2Simplex virus type 2Innate immune cellsT cell populationsAntiviral immune responseFemale genital tractPattern recognition receptorsVirus type 2Immune correlatesMemory CD4Memory CD8Viral clearanceHuman papillomavirusImmune cellsMemory lymphocytesSuccessful vaccineHIV-1Immune responseSevere diseaseVaginal epitheliumAdaptive immunityTarget cell type
2008
Dendritic cells and macrophages in the genitourinary tract
Iijima N, Thompson J, Iwasaki A. Dendritic cells and macrophages in the genitourinary tract. Mucosal Immunology 2008, 1: 451-459. PMID: 19079212, PMCID: PMC2684461, DOI: 10.1038/mi.2008.57.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsDendritic cellsGenitourinary tractGU tractUnderstanding of DCsSubsets of DCsImmune defenseFemale genital mucosaInnate immune defenseGenital mucosaMaternal toleranceSex hormonesAdaptive immunityReproductive functionMacrophagesTractNumerous agentsSTIsImportant reproductive functionsCellsDivergent rolesMucosaFetusesInfectionHormone
2004
MAdCAM-1 Expressing Sacral Lymph Node in the Lymphotoxin β-Deficient Mouse Provides a Site for Immune Generation Following Vaginal Herpes Simplex Virus-2 Infection
Soderberg KA, Linehan MM, Ruddle NH, Iwasaki A. MAdCAM-1 Expressing Sacral Lymph Node in the Lymphotoxin β-Deficient Mouse Provides a Site for Immune Generation Following Vaginal Herpes Simplex Virus-2 Infection. The Journal Of Immunology 2004, 173: 1908-1913. PMID: 15265924, DOI: 10.4049/jimmunol.173.3.1908.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralCD4-Positive T-LymphocytesCell Adhesion MoleculesDendritic CellsFemaleHerpes GenitalisHerpesvirus 2, HumanImmunoglobulin GImmunoglobulinsLymph NodesLymphocyte ActivationLymphotoxin-alphaLymphotoxin-betaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMucoproteinsSacrococcygeal RegionSplenectomyT-Cell Antigen Receptor SpecificityTh1 CellsVaginitisConceptsBeta-deficient miceSacral lymph nodesLymph nodesMesenteric lymph nodesWild-type miceGenital mucosaHerpes simplex virus 2 infectionIntravaginal HSV-2 infectionLT alpha-deficient miceMucosal addressin cell adhesion molecule-1Simplex virus 2 infectionCell adhesion molecule-1Mucosal lymph nodesAlpha-deficient miceCervical lymph nodesHSV-2 infectionVirus 2 infectionHSV type 2Potent immune responsesAdhesion molecule-1Intravaginal infectionTh1 responseDendritic cellsIgG responsesIliac arteryIn Vivo Role of Nectin-1 in Entry of Herpes Simplex Virus Type 1 (HSV-1) and HSV-2 through the Vaginal Mucosa
Linehan MM, Richman S, Krummenacher C, Eisenberg RJ, Cohen GH, Iwasaki A. In Vivo Role of Nectin-1 in Entry of Herpes Simplex Virus Type 1 (HSV-1) and HSV-2 through the Vaginal Mucosa. Journal Of Virology 2004, 78: 2530-2536. PMID: 14963155, PMCID: PMC369262, DOI: 10.1128/jvi.78.5.2530-2536.2004.Peer-Reviewed Original ResearchConceptsHSV-2HSV-1Viral entryNectin-1Genital herpesGenital mucosaVaginal epitheliumVaginal mucosaHerpes simplex virus type 2Simplex virus type 2Primary genital herpesHerpes simplex virus type 1Mouse vaginal epitheliumFemale genital tractSimplex virus type 1Virus type 1Virus type 2Intravaginal inoculationHSV infectionMenstrual cycleVaginal infectionsGenital tractMouse modelEstrous cycleType 2
2003
The role of dendritic cells in immune responses against vaginal infection by herpes simplex virus type 2
Iwasaki A. The role of dendritic cells in immune responses against vaginal infection by herpes simplex virus type 2. Microbes And Infection 2003, 5: 1221-1230. PMID: 14623018, DOI: 10.1016/j.micinf.2003.09.006.Peer-Reviewed Original ResearchConceptsHerpes simplex virus type 2Simplex virus type 2Virus type 2Dendritic cellsType 2Female genital mucosaSpecialized dendritic cellsEffector immunityGenital ulcersGenital herpesGenital infectionGenital mucosaProtective immunityLeading causeVaginal infectionsImmune responseAdaptive immunityMore womenImmunityRecent evidenceInfectionSpecific subsetCellsUlcersHerpes
2002
Expression of DC-SIGN by Dendritic Cells of Intestinal and Genital Mucosae in Humans and Rhesus Macaques
Jameson B, Baribaud F, Pöhlmann S, Ghavimi D, Mortari F, Doms RW, Iwasaki A. Expression of DC-SIGN by Dendritic Cells of Intestinal and Genital Mucosae in Humans and Rhesus Macaques. Journal Of Virology 2002, 76: 1866-1875. PMID: 11799181, PMCID: PMC135921, DOI: 10.1128/jvi.76.4.1866-1875.2002.Peer-Reviewed Original ResearchConceptsDC-SIGN expressionDendritic cellsDC-SIGNHuman immunodeficiency virus (HIV) transmissionRhesus macaquesDC-SIGNR expressionDistinct DC populationsSolitary lymphoid nodulesSubepithelial dendritic cellsSubepithelial dome regionTriple colour analysisPlasmacytoid dendritic cellsVirus transmissionDC-SIGNRIntact vaginal mucosaSpecific ICAM-3DC populationsHIV coreceptorsGenital mucosaLangerhans cellsPeyer's patchesGenital tractTerminal ileumInterfollicular regionsVaginal epithelium