2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivityWhy does Japan have so few cases of COVID‐19?
Iwasaki A, Grubaugh ND. Why does Japan have so few cases of COVID‐19? EMBO Molecular Medicine 2020, 12: emmm202012481. PMID: 32275804, PMCID: PMC7207161, DOI: 10.15252/emmm.202012481.Peer-Reviewed Original Research
2006
Dual recognition of herpes simplex viruses by TLR2 and TLR9 in dendritic cells
Sato A, Linehan MM, Iwasaki A. Dual recognition of herpes simplex viruses by TLR2 and TLR9 in dendritic cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 17343-17348. PMID: 17085599, PMCID: PMC1859932, DOI: 10.1073/pnas.0605102103.Peer-Reviewed Original ResearchConceptsToll-like receptorsHerpes simplex virusMultiple Toll-like receptorsDendritic cellsSimplex virusBone marrow-derived dendritic cellsMarrow-derived dendritic cellsMultiple pathogen-associated molecular patternsPrimary clinical isolatesIL-12 secretionConventional dendritic cellsSame dendritic cellInnate immune systemPathogen-associated molecular patternsViral infection resultsProinflammatory cytokinesSurface TLR2IL-6Infection resultsTLR2TLR9Viral recognitionImmune systemClinical isolatesAdditional agonists