2000
Binding of the human papillomavirus type 16 E7 oncoprotein and the adeno-associated virus Rep78 major regulatory protein in vitro and in yeast and the potential for downstream effects.
Hermonat PL, Santin AD, Zhan D. Binding of the human papillomavirus type 16 E7 oncoprotein and the adeno-associated virus Rep78 major regulatory protein in vitro and in yeast and the potential for downstream effects. Human Virology 2000, 3: 113-24. PMID: 10881991.Peer-Reviewed Original ResearchMeSH KeywordsAlanineBinding SitesBlotting, WesternChromatography, AffinityDependovirusDNA PrimersDNA-Binding ProteinsDNA, ComplementaryDNA, ViralHumansOncogene Proteins, ViralPapillomaviridaePapillomavirus E7 ProteinsProtein BindingRecombinant Fusion ProteinsTATA-Binding Protein Associated FactorsTATA-Box Binding ProteinTranscription Factor TFIIDTranscription FactorsViral ProteinsYeastsConceptsAAV life cycleHuman papillomavirusTerminal repeat DNACompetitive affinity chromatographyCancer-promoting activityRep78E7 oncoproteinsAAVRep78 proteinDownstream effectsHPV-16 E7 oncoproteinSeries of aminoHuman papillomavirus type 16 E7 oncoproteinHPV 16/18Cervical cancerHPV 16Variety of proteinsType 1Bovine papillomavirus type 1P97 promoterOncogenic transformationPapillomavirus type 1AdenoPotential downstream effectsDosage-dependent mannerTransduction and Utility of the Granulocyte-Macrophage Colony-Stimulating Factor Gene into Monocytes and Dendritic Cells by Adeno-Associated Virus
Liu Y, Santin AD, Mane M, Chiriva-Internati M, Parham GP, Ravaggi A, Hermonat PL. Transduction and Utility of the Granulocyte-Macrophage Colony-Stimulating Factor Gene into Monocytes and Dendritic Cells by Adeno-Associated Virus. Journal Of Interferon & Cytokine Research 2000, 20: 21-30. PMID: 10670649, DOI: 10.1089/107999000312702.Peer-Reviewed Original ResearchConceptsDendritic cellsMature dendritic cellsIL-4GM-CSFAntigen-presenting dendritic cellsAdherent monocytesTumor necrosis factor-alpha treatmentGranulocyte-macrophage colony-stimulating factorGM-CSF secretionInterleukin-4 treatmentColony-stimulating factorExogenous GM-CSFGranulocyte-macrophage colonyAntiviral protocolsDC precursorsAAV vectorsImmune response
1999
Binding of the Human Papillomavirus Type 16 p97 Promoter by the Adeno-associated Virus Rep78 Major Regulatory Protein Correlates with Inhibition*
Zhan D, Santin A, Liu Y, Parham G, Li C, Meyers C, Hermonat P. Binding of the Human Papillomavirus Type 16 p97 Promoter by the Adeno-associated Virus Rep78 Major Regulatory Protein Correlates with Inhibition*. Journal Of Biological Chemistry 1999, 274: 31619-31624. PMID: 10531369, DOI: 10.1074/jbc.274.44.31619.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBovine papillomavirus 1Cell Transformation, NeoplasticCell Transformation, ViralChromatography, AffinityColony-Forming Units AssayDependovirusDNA-Binding ProteinsGene Expression Regulation, ViralMolecular Sequence DataMutationOncogene Proteins, ViralPapillomaviridaePromoter Regions, GeneticProtein BindingRepetitive Sequences, Nucleic AcidRepressor ProteinsViral ProteinsChromosomal latency and expression at map unit 96 of a wild-type plus adeno-associated virus (AAV)/Neo vector and identification of p81, a new AAV transcriptional promoter.
Hermonat PL, Santin AD, De Greve J, De Rijcke M, Bishop BM, Han L, Mane M, Kokorina N. Chromosomal latency and expression at map unit 96 of a wild-type plus adeno-associated virus (AAV)/Neo vector and identification of p81, a new AAV transcriptional promoter. Human Virology 1999, 2: 359-68. PMID: 10774553.Peer-Reviewed Original Research
1998
Inhibition/Stimulation of Bovine Papillomavirus by Adeno-Associated Virus Is Time as Well as Multiplicity Dependent
Hermonat P, Meyers C, Parham G, Santin A. Inhibition/Stimulation of Bovine Papillomavirus by Adeno-Associated Virus Is Time as Well as Multiplicity Dependent. Virology 1998, 247: 240-250. PMID: 9705917, DOI: 10.1006/viro.1998.9256.Peer-Reviewed Original ResearchThe Adeno-Associated Virus Rep78 Major Regulatory Protein Binds the Cellular TATA-Binding Proteinin Vitroandin Vivo
Hermonat P, Santin A, Batchu R, Zhan D. The Adeno-Associated Virus Rep78 Major Regulatory Protein Binds the Cellular TATA-Binding Proteinin Vitroandin Vivo. Virology 1998, 245: 120-127. PMID: 9614873, DOI: 10.1006/viro.1998.9144.Peer-Reviewed Original ResearchConceptsPossible protein-protein interactionsBasal transcription factorsMajor regulatory proteinTranscription factorsRegulatory proteinsTwo-hybrid systemTranscription initiation complexProtein-protein interactionsNumerous interactionsYeast GAL4Human TATAInitiation complexHeterologous promoterAmino halfRep78Biological roleGene expressionWestern analysisVivo interactionAAV promotersPromoterTATAProteinElectrophoretic mobilityRegulatory abilityInvolvement of protein-DNA interaction in adeno-associated virus Rep78-mediated inhibition of HIV-1.
Kokorina NA, Santin AD, Li C, Hermonat PL. Involvement of protein-DNA interaction in adeno-associated virus Rep78-mediated inhibition of HIV-1. Human Virology 1998, 1: 441-50. PMID: 10195265.Peer-Reviewed Original ResearchConceptsProtein-DNA interactionsDNA binding abilityGene expressionHIV-1 long terminal repeat (LTR) sequencesTATA box regionLong terminal repeat sequencesHIV-LTR DNATerminal repeat sequencesRep78 proteinTranscription initiationTranscription factorsTATA boxRepeat sequencesVirus Rep78Rep78Box regionAmino acidsDNAInhibition of TatLikely targetMechanism of inhibitionMutantsHIV LTRElectrophoretic mobilityBinding
1997
Multiple cellular proteins are recognized by the adeno‐associated virus Rep78 major regulatory protein and the amino‐half of Rep78 is required for many of these interactions
Hermonat P, Carter C, Parham G, Quirk J, Santin A. Multiple cellular proteins are recognized by the adeno‐associated virus Rep78 major regulatory protein and the amino‐half of Rep78 is required for many of these interactions. IUBMB Life 1997, 43: 409-420. PMID: 9350349, DOI: 10.1080/15216549700204201.Peer-Reviewed Original ResearchConceptsMultiple cellular proteinsCellular proteinsHeterologous gene expressionHuman chromosome 19Multi-functional proteinMajor regulatory proteinSite-specific integrationAAV DNA replicationLarge T antigenRep78 proteinReplication proteinsDNA replicationProtein interactionsRegulatory proteinsTranscriptional levelChromosome 19Rep78Assay systemGene expressionCellular extractsT antigenProteinAAV DNASpecific integrationCoimmunoprecipitationAdeno-Associated Virus Rep78 Inhibits Oncogenic Transformation of Primary Human Keratinocytes by a Human Papillomavirus Type 16-rasChimeric
Hermonat PL, Plott RT, Santin AD, Parham GP, Flick JT. Adeno-Associated Virus Rep78 Inhibits Oncogenic Transformation of Primary Human Keratinocytes by a Human Papillomavirus Type 16-rasChimeric. Gynecologic Oncology 1997, 66: 487-494. PMID: 9299265, DOI: 10.1006/gyno.1997.4789.Peer-Reviewed Original ResearchConceptsHuman papillomavirusCervical cancerCervical carcinogenesisPapillomavirus typesHPV 16/18 infectionHuman papillomavirus typesHuman papillomavirus type 16Primary human foreskin keratinocytesCell typesHPV-16 p97 promoterPapillomavirus type 16Ability of AAVPrimary human keratinocytesSeroepidemiologic studiesInverse associationHuman foreskin keratinocytesVirus infectionType 16Host cell typesTransient CAT assaysType 1P97 promoterOncogenic transformationForeskin keratinocytesHuman keratinocytes
1996
Role of the terminal repeat GAGC trimer, the major Rep78 binding site, in adeno‐associated virus DNA replication
Bishop BM, Santin AD, Quirk JG, Hermonat PL. Role of the terminal repeat GAGC trimer, the major Rep78 binding site, in adeno‐associated virus DNA replication. FEBS Letters 1996, 397: 97-100. PMID: 8941722, DOI: 10.1016/s0014-5793(96)01149-0.Peer-Reviewed Original Research