2018
Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
Montemayor E, Didychuk A, Yake A, Sidhu G, Brow D, Butcher S. Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA. Nature Communications 2018, 9: 1749. PMID: 29717126, PMCID: PMC5931518, DOI: 10.1038/s41467-018-04145-4.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNAU6 snRNPPre-mRNA substratePrecursor messenger RNAProtein-protein contactsC-terminal regionSaccharomyces cerevisiaeHeteroheptameric ringMature mRNAActive siteU6 snRNPsMRNA decayNuclear RNAPost-transcriptionallySpliceosomeLsm2Prp24SnRNPMessenger RNARNAMRNAIntronLSm8
2017
Structural Characterization of the Recognition of U6 snRNA by the Yeast U6 Biogenesis Protein Usb1
DeLaitsch A, Didychuk A, Montemayor E, Larson M, Lucarelli S, Butcher S. Structural Characterization of the Recognition of U6 snRNA by the Yeast U6 Biogenesis Protein Usb1. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.910.8.Peer-Reviewed Original ResearchU6 snRNAU6 RNAMutation of active site residuesTerminal phosphateDenaturing polyacrylamide gel electrophoresisBinding of RNACatalytically active spliceosomePrecursor messenger RNANon-coding intronsActive site residuesPolyacrylamide gel electrophoresisSnRNA biogenesisExoribonuclease activityS. cerevisiaeSequence identityActive spliceosomeEukaryotic cellsConserved UFluorescent RNADiffracted X-raysU6 snRNPActive siteProtein complexesSite residuesUSB1
2012
Structure of Pisum sativum Rubisco with bound ribulose 1,5‐bisphosphate
Loewen P, Didychuk A, Switala J, Perez-Luque R, Fita I, Loewen M. Structure of Pisum sativum Rubisco with bound ribulose 1,5‐bisphosphate. Acta Crystallographica Section F: Structural Biology Communications 2012, 69: 10-14. PMID: 23295478, PMCID: PMC3539695, DOI: 10.1107/s1744309112047549.Peer-Reviewed Original ResearchConceptsRibulose 1,5-bisphosphateRibulose-1,5-bisphosphate carboxylase/oxygenaseSubstrate ribulose 1,5-bisphosphateHanging-drop vapor diffusionSmall subunit sequenceRibulose-1,5-bisphosphateDiffraction-quality crystalsActive-site conformationActive siteRubisco structureRubiscoClosed conformationHexadecameric complexPisum sativumA-resolutionQuaternary structureGarden peaVapor diffusionX-ray diffraction dataPulse cropsCarboxylase/oxygenaseLys201ConformationSitesDiffraction data