2016
A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex
Rodgers M, Didychuk A, Butcher S, Brow D, Hoskins A. A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex. Nucleic Acids Research 2016, 44: 10912-10928. PMID: 27484481, PMCID: PMC5159527, DOI: 10.1093/nar/gkw686.Peer-Reviewed Original ResearchConceptsSmall nuclear RNATri-snRNPU4/U6 unwindingU6 small nuclear RNAImpairs yeast growthSpliceosome active siteU4/U6 di-snRNPSmall nuclear ribonucleoproteinTri-snRNP assemblySpliceosome formationStrand invasionDi-snRNPYeast growthNuclear ribonucleoproteinNuclear RNARNA complexResonance energy transferSpliceosomeRNA duplexesForster resonance energy transferConformational rearrangementsDNA oligonucleotidesStem IIUnwindingU4/U6
2015
Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly
Didychuk A, Montemayor E, Brow D, Butcher S. Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly. Nucleic Acids Research 2015, 44: 1398-1410. PMID: 26673715, PMCID: PMC4756825, DOI: 10.1093/nar/gkv1374.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding, CompetitiveKineticsModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationProtein BindingProtein Structure, TertiaryRibonucleoprotein, U4-U6 Small NuclearRibonucleoproteins, Small NuclearRNA, FungalRNA, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsU6 RNADi-snRNPRemodeling of RNA structureAnnealing in vitroTri-snRNP complexLarge-scale remodelingStable ternary complexRNA bindingRNA structureRibonucleoprotein complexU6 snRNPU6 snRNABase pairsPrp24RNATernary complexU4/U6Electropositive characterRate enhancementAssemblyStructural requirementsSnRNASnRNPLsm2Annealing rate