2018
Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
Montemayor E, Didychuk A, Yake A, Sidhu G, Brow D, Butcher S. Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA. Nature Communications 2018, 9: 1749. PMID: 29717126, PMCID: PMC5931518, DOI: 10.1038/s41467-018-04145-4.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNAU6 snRNPPre-mRNA substratePrecursor messenger RNAProtein-protein contactsC-terminal regionSaccharomyces cerevisiaeHeteroheptameric ringMature mRNAActive siteU6 snRNPsMRNA decayNuclear RNAPost-transcriptionallySpliceosomeLsm2Prp24SnRNPMessenger RNARNAMRNAIntronLSm8
2017
Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core
Montemayor E, Didychuk A, Liao H, Hu P, Brow D, Butcher S. Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core. Acta Crystallographica Section D, Structural Biology 2017, 73: 1-8. PMID: 28045380, PMCID: PMC5331471, DOI: 10.1107/s2059798316018222.Peer-Reviewed Original ResearchInternal stem-loopRNA recognition motifSmall nuclear ribonucleoproteinU6 internal stem-loopSplicing of precursor messenger RNAU6 small nuclear RNAProtein-RNA interfacesU6 small nuclear ribonucleoproteinsPrecursor messenger RNASmall nuclear RNAGel shift assaysStem-loopRibonucleoprotein complexCrystal structureGenetic dataNuclear ribonucleoproteinNuclear RNAConformational plasticityWild-typePrp24RNASpliceosomeElectrophoretic mobilityMessenger RNARibonucleoprotein