2018
Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
Montemayor E, Didychuk A, Yake A, Sidhu G, Brow D, Butcher S. Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA. Nature Communications 2018, 9: 1749. PMID: 29717126, PMCID: PMC5931518, DOI: 10.1038/s41467-018-04145-4.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNAU6 snRNPPre-mRNA substratePrecursor messenger RNAProtein-protein contactsC-terminal regionSaccharomyces cerevisiaeHeteroheptameric ringMature mRNAActive siteU6 snRNPsMRNA decayNuclear RNAPost-transcriptionallySpliceosomeLsm2Prp24SnRNPMessenger RNARNAMRNAIntronLSm8The life of U6 small nuclear RNA, from cradle to grave
Didychuk A, Butcher S, Brow D. The life of U6 small nuclear RNA, from cradle to grave. RNA 2018, 24: 437-460. PMID: 29367453, PMCID: PMC5855946, DOI: 10.1261/rna.065136.117.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNANuclear RNAProcess of RNA splicingCatalyzes intron removalEukaryotic gene expressionPre-mRNA substrateUridine-rich small nuclear RNAsRemoval of intronsPrecursor messenger RNACryo-EM structureSplicing cycleNoncoding transcriptsCatalytic coreProtein partnersRNA splicingIntron removalSplice siteGenetic dataMacromolecular machinesSpliceosomeGene expressionSplicingConformational changes
2017
Structural Characterization of the Recognition of U6 snRNA by the Yeast U6 Biogenesis Protein Usb1
DeLaitsch A, Didychuk A, Montemayor E, Larson M, Lucarelli S, Butcher S. Structural Characterization of the Recognition of U6 snRNA by the Yeast U6 Biogenesis Protein Usb1. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.910.8.Peer-Reviewed Original ResearchU6 snRNAU6 RNAMutation of active site residuesTerminal phosphateDenaturing polyacrylamide gel electrophoresisBinding of RNACatalytically active spliceosomePrecursor messenger RNANon-coding intronsActive site residuesPolyacrylamide gel electrophoresisSnRNA biogenesisExoribonuclease activityS. cerevisiaeSequence identityActive spliceosomeEukaryotic cellsConserved UFluorescent RNADiffracted X-raysU6 snRNPActive siteProtein complexesSite residuesUSB1Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core
Montemayor E, Didychuk A, Liao H, Hu P, Brow D, Butcher S. Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core. Acta Crystallographica Section D, Structural Biology 2017, 73: 1-8. PMID: 28045380, PMCID: PMC5331471, DOI: 10.1107/s2059798316018222.Peer-Reviewed Original ResearchInternal stem-loopRNA recognition motifSmall nuclear ribonucleoproteinU6 internal stem-loopSplicing of precursor messenger RNAU6 small nuclear RNAProtein-RNA interfacesU6 small nuclear ribonucleoproteinsPrecursor messenger RNASmall nuclear RNAGel shift assaysStem-loopRibonucleoprotein complexCrystal structureGenetic dataNuclear ribonucleoproteinNuclear RNAConformational plasticityWild-typePrp24RNASpliceosomeElectrophoretic mobilityMessenger RNARibonucleoprotein