2018
Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
Montemayor E, Didychuk A, Yake A, Sidhu G, Brow D, Butcher S. Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA. Nature Communications 2018, 9: 1749. PMID: 29717126, PMCID: PMC5931518, DOI: 10.1038/s41467-018-04145-4.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNAU6 snRNPPre-mRNA substratePrecursor messenger RNAProtein-protein contactsC-terminal regionSaccharomyces cerevisiaeHeteroheptameric ringMature mRNAActive siteU6 snRNPsMRNA decayNuclear RNAPost-transcriptionallySpliceosomeLsm2Prp24SnRNPMessenger RNARNAMRNAIntronLSm8The life of U6 small nuclear RNA, from cradle to grave
Didychuk A, Butcher S, Brow D. The life of U6 small nuclear RNA, from cradle to grave. RNA 2018, 24: 437-460. PMID: 29367453, PMCID: PMC5855946, DOI: 10.1261/rna.065136.117.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNANuclear RNAProcess of RNA splicingCatalyzes intron removalEukaryotic gene expressionPre-mRNA substrateUridine-rich small nuclear RNAsRemoval of intronsPrecursor messenger RNACryo-EM structureSplicing cycleNoncoding transcriptsCatalytic coreProtein partnersRNA splicingIntron removalSplice siteGenetic dataMacromolecular machinesSpliceosomeGene expressionSplicingConformational changes
2017
Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities
Didychuk A, Montemayor E, Carrocci T, DeLaitsch A, Lucarelli S, Westler W, Brow D, Hoskins A, Butcher S. Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities. Nature Communications 2017, 8: 497. PMID: 28887445, PMCID: PMC5591277, DOI: 10.1038/s41467-017-00484-w.Peer-Reviewed Original ResearchMeSH KeywordsCatalytic DomainCrystallography, X-RayEvolution, MolecularGenetic VariationHumansModels, MolecularPhosphoric Diester HydrolasesProtein BindingProtein DomainsRibonucleoprotein, U4-U6 Small NuclearRNA, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSubstrate SpecificityConceptsU6 snRNP assemblySmall nuclear ribonucleoproteinSnRNP assemblyCognate RNA-binding proteinsTerminal 3'-phosphateU6 small nuclear ribonucleoproteinsRNA-binding proteinsAnti-cooperative interactionsCyclic phosphodiesterase activitySpliceosome assemblyU6 RNAHuman orthologNuclear ribonucleoproteinUSB1SpliceosomeYeastProteinPhosphodiesterase activityAntagonist proteinComplex seriesAssemblyPrp24LHP1OrthologsSnRNA
2016
A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex
Rodgers M, Didychuk A, Butcher S, Brow D, Hoskins A. A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex. Nucleic Acids Research 2016, 44: 10912-10928. PMID: 27484481, PMCID: PMC5159527, DOI: 10.1093/nar/gkw686.Peer-Reviewed Original ResearchMeSH KeywordsBase PairingKineticsRNA StabilityRNA, Double-StrandedRNA, FungalRNA, Small NuclearSaccharomyces cerevisiaeConceptsSmall nuclear RNATri-snRNPU4/U6 unwindingU6 small nuclear RNAImpairs yeast growthSpliceosome active siteU4/U6 di-snRNPSmall nuclear ribonucleoproteinTri-snRNP assemblySpliceosome formationStrand invasionDi-snRNPYeast growthNuclear ribonucleoproteinNuclear RNARNA complexResonance energy transferSpliceosomeRNA duplexesForster resonance energy transferConformational rearrangementsDNA oligonucleotidesStem IIUnwindingU4/U6
2015
Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly
Didychuk A, Montemayor E, Brow D, Butcher S. Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly. Nucleic Acids Research 2015, 44: 1398-1410. PMID: 26673715, PMCID: PMC4756825, DOI: 10.1093/nar/gkv1374.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding, CompetitiveKineticsModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationProtein BindingProtein Structure, TertiaryRibonucleoprotein, U4-U6 Small NuclearRibonucleoproteins, Small NuclearRNA, FungalRNA, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsU6 RNADi-snRNPRemodeling of RNA structureAnnealing in vitroTri-snRNP complexLarge-scale remodelingStable ternary complexRNA bindingRNA structureRibonucleoprotein complexU6 snRNPU6 snRNABase pairsPrp24RNATernary complexU4/U6Electropositive characterRate enhancementAssemblyStructural requirementsSnRNASnRNPLsm2Annealing rateStructural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA
Cornilescu G, Didychuk A, Rodgers M, Michael L, Burke J, Montemayor E, Hoskins A, Butcher S. Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal Of Molecular Biology 2015, 428: 777-789. PMID: 26655855, PMCID: PMC4790120, DOI: 10.1016/j.jmb.2015.11.026.Peer-Reviewed Original ResearchConceptsU4/U6 di-snRNAScattering dataTri-snRNPAnalysis of RNA structureCryo-electron microscopy structureResonance energy transfer dataX-ray scattering dataMagnetic susceptibility anisotropyRNA structureAssembled spliceosomeRibonucleoprotein complexTertiary interactionsU4/U6Energy transfer dataAlignment mediaSusceptibility anisotropyCryo-electronAlignment tensorStructural investigationsRNAX-rayNucleic acidsStructural analysisWAXS dataY-shaped structure