2023
The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection
McCollum C, Didychuk A, Liu D, Murray-Nerger L, Cristea I, Glaunsinger B. The viral packaging motor potentiates Kaposi’s sarcoma-associated herpesvirus gene expression late in infection. PLOS Pathogens 2023, 19: e1011163. PMID: 37068108, PMCID: PMC10138851, DOI: 10.1371/journal.ppat.1011163.Peer-Reviewed Original ResearchMeSH KeywordsGene ExpressionGene Expression Regulation, ViralHerpesvirus 8, HumanViral Genome PackagingViral ProteinsVirus ReplicationConceptsKaposi's sarcoma-associated herpesvirusGene expressionViral transcriptionKSHV gene expressionCellular RNA polymerasesHerpesvirus gene expressionTranscriptional activator proteinViral DNA packaging motorsPotential gene expressionTranscriptional activation complexLate gene expressionDNA packaging motorRNA polymeraseProximity labelingHost transcriptionSiRNA screenSarcoma-associated herpesvirusLate genesTranscription complexLate proteinsCatalytic subunitActivator proteinPackaging motorORF29Viral packaging
2022
DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production
Hartenian E, Mendez A, Didychuk A, Khosla S, Glaunsinger B. DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production. Nucleic Acids Research 2022, 51: 182-197. PMID: 36537232, PMCID: PMC9841436, DOI: 10.1093/nar/gkac1190.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA, ViralExonucleasesGene ExpressionGene Expression Regulation, ViralHerpesvirus 8, HumanHumansMammalsVirionVirus ReplicationConceptsKaposi's sarcoma-associated herpesvirusAlkaline exonucleaseInfectious virion productionViral gene expressionDNA processingStructure-guided functional analysesGene expressionDNA substrate preferenceVirion productionKingdoms of lifeCleavage of mRNAGammaherpesvirus Kaposi's sarcoma-associated herpesvirusViral DNA processingLifecycles of virusesBacteriophage LSoxS mutantFunctional conservationGenome replicationGenetic conservationSarcoma-associated herpesvirusPhage LSubstrate preferenceDNA bindingHuman gammaherpesviruses Kaposi's sarcoma-associated herpesvirusSOX activity
2021
A pentameric protein ring with novel architecture is required for herpesviral packaging
Didychuk A, Gates S, Gardner M, Strong L, Martin A, Glaunsinger B. A pentameric protein ring with novel architecture is required for herpesviral packaging. ELife 2021, 10: e62261. PMID: 33554858, PMCID: PMC7889075, DOI: 10.7554/elife.62261.Peer-Reviewed Original ResearchConceptsViral genomeAccessory factorsBind double-stranded DNAPositively charged central channelContext of KSHV infectionDouble-stranded DNA virusesPositively charged residuesOncogenic herpesvirus Kaposi's sarcoma-associated herpesvirusKaposi's sarcoma-associated herpesvirusDouble-stranded DNASarcoma-associated herpesvirusGenome packagingHomologous proteinsNascent capsidsDNA bindingProtein ringGenomePackaging motorDNA virusesCharged residuesProgeny virionsMolecular motorsCentral channelKSHV infectionMutants