2022
A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains
Morgens D, Nandakumar D, Didychuk A, Yang K, Glaunsinger B. A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains. PLOS Pathogens 2022, 18: e1010236. PMID: 35041709, PMCID: PMC8797222, DOI: 10.1371/journal.ppat.1010236.Peer-Reviewed Original ResearchMeSH KeywordsCRISPR-Cas SystemsGene Expression Regulation, ViralGenes, ViralHEK293 CellsHerpesvirus 8, HumanHumansConceptsViral DNA replicationKaposi's sarcoma-associated herpesvirusViral genomeDsDNA virusesLate genesDNA replicationTranscriptional activityViral sequencesViral transcriptional activityCatalytic domainIndividual mutantsSgRNA librarySarcoma-associated herpesvirusTiling screensDNA bindingDeep sequencingBase pairsFunctional screeningCut sitePooled screeningTranscriptional modifiersDNA virusesGenomeEssential domainsGene expression
2021
A pentameric protein ring with novel architecture is required for herpesviral packaging
Didychuk A, Gates S, Gardner M, Strong L, Martin A, Glaunsinger B. A pentameric protein ring with novel architecture is required for herpesviral packaging. ELife 2021, 10: e62261. PMID: 33554858, PMCID: PMC7889075, DOI: 10.7554/elife.62261.Peer-Reviewed Original ResearchConceptsViral genomeAccessory factorsBind double-stranded DNAPositively charged central channelContext of KSHV infectionDouble-stranded DNA virusesPositively charged residuesOncogenic herpesvirus Kaposi's sarcoma-associated herpesvirusKaposi's sarcoma-associated herpesvirusDouble-stranded DNASarcoma-associated herpesvirusGenome packagingHomologous proteinsNascent capsidsDNA bindingProtein ringGenomePackaging motorDNA virusesCharged residuesProgeny virionsMolecular motorsCentral channelKSHV infectionMutants
2020
The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
Castañeda A, Didychuk A, Louder R, McCollum C, Davis Z, Nogales E, Glaunsinger B. The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription. PLOS Pathogens 2020, 16: e1008843. PMID: 32886723, PMCID: PMC7498053, DOI: 10.1371/journal.ppat.1008843.Peer-Reviewed Original ResearchConceptsTATA box-binding proteinRNA polymerase IIN-terminal domainPol IIPolymerase IICellular TATA box binding proteinHost RNA polymerase IIRecruitment of RNA polymerase IIGene transcriptionLate gene transcriptionPol II recruitmentProtein interaction studiesProtein-protein contactsC-terminal domainEukaryotic transcriptionPolymerase recruitmentHuman cytomegalovirusPreinitiation complexHost transcriptionRNA PolLate genesMicroscopy-based imagingKaposi's sarcoma-associated virusTranscriptional activityPromoter recognitionConserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34
Didychuk A, Castañeda A, Kushnir L, Huang C, Glaunsinger B. Conserved CxnC Motifs in Kaposi’s Sarcoma-Associated Herpesvirus ORF66 Are Required for Viral Late Gene Expression and Are Essential for Its Interaction with ORF34. Journal Of Virology 2020, 94: 10.1128/jvi.01299-19. PMID: 31578296, PMCID: PMC6955276, DOI: 10.1128/jvi.01299-19.Peer-Reviewed Original ResearchConceptsViral preinitiation complexKaposi's sarcoma-associated herpesvirusC-terminal domainCysteine-rich motifHost transcription machineryPreinitiation complexGene promoterTranscription machinerySarcoma-associated herpesvirusLate genesGene transcriptionViral late gene expressionZinc finger motifsLate gene transcriptionSequence-specific bindingTranscriptional regulatory activityLate gene expressionLate gene promotersInfectious virionsProduction of capsid proteinsRelease of infectious virionsViral replication cycleFinger motifPromoter occupancySequence-specific