2024
Spatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification
Kang T, Bocci F, Nie Q, Onuchic J, Levchenko A. Spatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification. ELife 2024, 12: rp89262. PMID: 38376371, PMCID: PMC10942579, DOI: 10.7554/elife.89262.Peer-Reviewed Original ResearchConceptsCell fate specificationFate specificationNotch signalingMorphogenic processesCell-cell communicationComplex morphogenic processesCell fateDynamics of spatial patternsDepletion of fibronectinTip cellsSprout extensionAngiogenic morphogenesisHypoxic micro-environmentCell plasticityCellsComputational analysisPre-existing onesCell patternMicro-environmentSpatial patternsLocal enrichmentMorphogenesisEndothelial cellsAngiogenesis modelFibronectin
2022
Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells
Kshitiz, Afzal J, Suhail Y, Chang H, Hubbi M, Hamidzadeh A, Goyal R, Liu Y, Sun P, Nicoli S, Dang C, Levchenko A. Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells. Cell Systems 2022, 13: 1048-1064.e7. PMID: 36462504, PMCID: PMC10012408, DOI: 10.1016/j.cels.2022.11.003.Peer-Reviewed Original ResearchConceptsHIF-1α activityActivation of genesChaperone-mediated autophagyCancer cellsCell divisionIndividual cancer cellsRegulated processPatient-derived cancer cellsSubset of cellsMolecular mechanismsFluorescent reportersSingle-cell responsesCancer cell linesCell linesGenesHypoxic tumor cellsHIF-1αHypoxic conditionsCellsBroad suppressionAggressive growthTumor cellsHypoxic cellsOscillatory activityExtracellular lactate
2009
Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy
Kim D, Seo C, Han K, Kwon K, Levchenko A, Suh K. Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy. Advanced Functional Materials 2009, 19: 1579-1586. DOI: 10.1002/adfm.200801174.Peer-Reviewed Original ResearchVariable local densityCell culture substratesCell elongationCell-material interfaceCell shapeMigration of cellsCell migrationCell morphologyCulture substrateMicrotextured SubstratesAdvanced tissue engineeringCell orientationCellsSingle experimentUV-assisted capillary force lithographyTissue engineeringSubstrate
2006
Micro Fluidic Chemostat with Deformable Membranes: Intracellular Biofilm-Like Structure Model
Cho H, Groisman A, Campbell J, Flores S, Levchenko A. Micro Fluidic Chemostat with Deformable Membranes: Intracellular Biofilm-Like Structure Model. 2006, 96-96. DOI: 10.1109/bmn.2006.330906.Peer-Reviewed Original ResearchHost cell membraneMicrofluidic chemostatBacterial cell-cell communicationCell-cell communicationCell membraneIndividual cell behaviorsGene expressionStress responseProtein techniquesHigh-density coloniesBiological stressCell behaviorEscherichia coliBiofilm formationBacterial biofilmsHost organsIntriguing questionChemostatBacterial studiesPathogenic microbiologyMembraneBiofilmsExperimental toolCellsExact mechanismMicrofluidic Chemostat with Deformable Membranes: Intracellular Biofilm-Like Structure Model
Cho H, Groisman A, Campbell J, Flores S, Levchenko A. Microfluidic Chemostat with Deformable Membranes: Intracellular Biofilm-Like Structure Model. 2006, 14-14. DOI: 10.1109/bmn.2006.330900.Peer-Reviewed Original ResearchMicrofluidic chemostatHost cell membraneBacterial cell-cell communicationCell-cell communicationCell membraneIndividual cell behaviorsGene expressionStress responseProtein techniquesHigh-density coloniesBiological stressCell behaviorEscherichia coliBiofilm formationBacterial biofilmsHost organsIntriguing questionChemostatBacterial studiesPathogenic microbiologyMembraneBiofilmsExperimental toolCellsExact mechanism
2005
Intercellular transfer of P-glycoprotein mediates acquired multidrug resistance in tumor cells
Levchenko A, Mehta B, Niu X, Kang G, Villafania L, Way D, Polycarpe D, Sadelain M, Larson S. Intercellular transfer of P-glycoprotein mediates acquired multidrug resistance in tumor cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 1933-1938. PMID: 15671173, PMCID: PMC545583, DOI: 10.1073/pnas.0401851102.Peer-Reviewed Original ResearchConceptsFunctional P-gpP-gpTumor cellsMultidrug resistanceP-gp-mediated resistanceP-gp-negative cellsToxic drug concentrationsP-gp-positive cellsIntercellular transferResistant tumor cellsDifferent tumor cell typesTumor cell typesTumor massTumor stromaP-gp transferTumor typesP-glycoproteinStromal elementsDrug resistanceDrug concentrationsTumor samplesBiologic propertiesCancer therapyCell typesCells
2004
The Systems Biology of Glycosylation
Murrell M, Yarema K, Levchenko A. The Systems Biology of Glycosylation. ChemBioChem 2004, 5: 1334-1347. PMID: 15457533, DOI: 10.1002/cbic.200400143.Peer-Reviewed Original ResearchConceptsSystems biologyRegulation of differentiationRegulation of glycosylationEukaryotic cellsBiochemical systems analysisCell regulation processesCell decisionsSignal transductionGlycosylationBiologyRegulation processesRegulationTransductionApoptosisDifferentiationProfound influenceCellsNovel research methodology
2003
Allovalency: A Case of Molecular Entanglement
Levchenko A. Allovalency: A Case of Molecular Entanglement. Current Biology 2003, 13: r876-r878. PMID: 14614843, DOI: 10.1016/j.cub.2003.10.049.Peer-Reviewed Original Research
2001
A general framework for achieving integral control in chemotactic biological signaling mechanisms
Iglesias P, Levchenko A. A general framework for achieving integral control in chemotactic biological signaling mechanisms. 2001, 1: 843-848 vol.1. DOI: 10.1109/cdc.2001.980211.Peer-Reviewed Original Research