2020
RNA-based CRISPR-Mediated Loss-of-Function Mutagenesis in Human Pluripotent Stem Cells
Leung AW, Broton C, Bogacheva MS, Xiao AZ, Garcia-Castro MI, Lou YR. RNA-based CRISPR-Mediated Loss-of-Function Mutagenesis in Human Pluripotent Stem Cells. Journal Of Molecular Biology 2020, 432: 3956-3964. PMID: 32339532, DOI: 10.1016/j.jmb.2020.04.017.Peer-Reviewed Original ResearchConceptsHuman pluripotent stem cellsPluripotent stem cellsTargeting efficiencyShelf cell productsTransfection protocolShort palindromic repeatsStem cellsSelection markerGenome editingPS cell linesFunction mutagenesisImproved protocolBroad applicationsPalindromic repeatsClustered RegularlyAssociated 9Human therapyEfficiencyProtocolCell productsCRISPRCrRNARegularlyApplicationsEditing
2015
Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells
Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz N, Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban S, Buganim Y. Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells. Cell Stem Cell 2015, 17: 543-556. PMID: 26412562, DOI: 10.1016/j.stem.2015.08.006.Peer-Reviewed Original ResearchConceptsNuclear reprogrammingEmbryonic stem cellsStem-like cellsTransient pluripotent stateStem cellsDNA methylation profilesPluripotent stem cellsPluripotent stateLineage conversionTranscriptional profilesTransient expressionMethylation profilesReprogrammingEvidence of passageMouse fibroblastsPluripotencyChimera assaysLineagesCellsHigh degreeTranscriptomeTFAP2CMethylationDifferentiationEomes