Gene transfer via in vivo electroporation and in combination with electrophysiology – a novel and efficient high‐throughput assay to assess neuronal functionality
Huttner A, Kasten M, Anderson M. Gene transfer via in vivo electroporation and in combination with electrophysiology – a novel and efficient high‐throughput assay to assess neuronal functionality. The FASEB Journal 2007, 21: lb20-lb20. DOI: 10.1096/fasebj.21.6.lb20-a.Peer-Reviewed Original ResearchGene functionRecent large-scale screensCell type-specific expression patternsEmbryonic lethal phenotypeCell type-specific promotersMultitude of genesSpecific expression patternsLarge-scale screenHigh-throughput assaysCre/loxP technologyEmbryonic day 14.5Lethal phenotypeGenetic manipulationSpecific promotersMouse embryosExpression patternsSingle-cell patch-clamp recordingsIndividual cellsGene transferCNS developmentFluorescence microscopyDifferential interference contrastLoxP technologyTemporal controlCell morphology