2024
Covalent Polymer‐RNA Conjugates for Potent Activation of the RIG‐I Pathway
Palmer C, Pastora L, Kimmel B, Pagendarm H, Kwiatkowski A, Stone P, Arora K, Francini N, Fedorova O, Pyle A, Wilson J. Covalent Polymer‐RNA Conjugates for Potent Activation of the RIG‐I Pathway. Advanced Healthcare Materials 2024, e2303815. PMID: 38648653, PMCID: PMC11493851, DOI: 10.1002/adhm.202303815.Peer-Reviewed Original ResearchChemical design principlesActivation of RIG-I.Retinoic acid-inducible gene ICovalent conjugationRIG-I pathwayNuclease degradationThioether linkageRIG-I.Gene IRNA ligandsElectrostatic interactionsRIG-IDevelopment of therapeuticsPotent activityOligonucleotide therapeuticsNucleaseRNAAnalog carrierNanoparticlesDrug delivery barriersConjugatePolymeric carriersImmunostimulatory activityThioethersIn vivo delivery
2020
Structural Optimization of Polymeric Carriers to Enhance the Immunostimulatory Activity of Molecularly Defined RIG‑I Agonists
Jacobson ME, Becker KW, Palmer CR, Pastora LE, Fletcher RB, Collins KA, Fedorova O, Duvall CL, Pyle AM, Wilson JT. Structural Optimization of Polymeric Carriers to Enhance the Immunostimulatory Activity of Molecularly Defined RIG‑I Agonists. ACS Central Science 2020, 6: 2008-2022. PMID: 33274278, PMCID: PMC7706089, DOI: 10.1021/acscentsci.0c00568.Peer-Reviewed Original ResearchPolymeric carriersNew structure-activity relationshipsPH-responsive propertiesAlkyl methacrylate monomersIntracellular deliveryAlkyl chain lengthInefficient intracellular deliverySeries of PEGEndosomal escapeNovel polymeric carriersNucleic acid therapeuticsLead carrierStructure-activity relationshipsPolymer structureMethacrylate monomerPolymer compositionChain lengthPolymeric deliveryMonomer compositionPolymersMajor clearance organVariable compositionDiameter particlesMolecularlyCarriers