2023
Structural insights into intron catalysis and dynamics during splicing
Xu L, Liu T, Chung K, Pyle A. Structural insights into intron catalysis and dynamics during splicing. Nature 2023, 624: 682-688. PMID: 37993708, PMCID: PMC10733145, DOI: 10.1038/s41586-023-06746-6.Peer-Reviewed Original Research
2022
A molecular beacon assay for monitoring RNA splicing
Omran QQ, Fedorova O, Liu T, Pyle AM. A molecular beacon assay for monitoring RNA splicing. Nucleic Acids Research 2022, 50: e74-e74. PMID: 35438748, PMCID: PMC9303364, DOI: 10.1093/nar/gkac242.Peer-Reviewed Original ResearchConceptsMolecular beaconsMolecular beacon assayHigher fluorescent signalHigh-throughput screening platformSmall moleculesAutocatalyzed reactionMolecular beacon approachKinetic characterizationReactionSmall molecule targetingInhibition constantsScreening platformSmall molecule inhibitorsFluorescent signalMolecule targetingMolecule inhibitorsMolecules
2003
Domains 2 and 3 Interact to Form Critical Elements of the Group II Intron Active Site
Fedorova O, Mitros T, Pyle AM. Domains 2 and 3 Interact to Form Critical Elements of the Group II Intron Active Site. Journal Of Molecular Biology 2003, 330: 197-209. PMID: 12823961, DOI: 10.1016/s0022-2836(03)00594-1.Peer-Reviewed Original Research
1995
Branch-point attack in group II introns is a highly reversible transesterification, providing a potential proofreading mechanism for 5'-splice site selection.
Chin K, Pyle AM. Branch-point attack in group II introns is a highly reversible transesterification, providing a potential proofreading mechanism for 5'-splice site selection. RNA 1995, 1: 391-406. PMID: 7493317, PMCID: PMC1482411.Peer-Reviewed Original Research