1996
[16] Purification of yeast RNA polymerase II holoenzymes
Koleske AJ, Chao DM, Young RA. [16] Purification of yeast RNA polymerase II holoenzymes. Methods In Enzymology 1996, 273: 176-184. PMID: 8791611, DOI: 10.1016/s0076-6879(96)73018-5.Peer-Reviewed Original ResearchConceptsRNA polymerase II holoenzymeRNA polymerase IIPolymerase IIYeast RNA polymerase II holoenzymeGeneral transcription factors TBPTranscription factors TBPHigh molecular weight complexesMolecular weight complexesTranscriptional regulationTranscription initiationYeast SaccharomycesTranscription studiesHoloenzymeHoloenzyme preparationWeight complexesTranscriptionLow salt bufferExciting cluesLarge formSalt concentrationTFIIBTFIIEAssaysSaccharomycesPurification
1993
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
Thompson C, Koleske A, Chao D, Young R. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 1993, 73: 1361-1375. PMID: 8324825, DOI: 10.1016/0092-8674(93)90362-t.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesDNA Mutational AnalysisDNA-Binding ProteinsFungal ProteinsGene Expression RegulationMediator ComplexMolecular Sequence DataMultienzyme ComplexesRecombinant ProteinsRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTATA-Box Binding ProteinTranscription FactorsTranscription, GeneticConceptsTATA-binding proteinRNA polymerase II carboxy-terminal domainCarboxy-terminal domainMultisubunit complexLarge multisubunit complexFunctional preinitiation complexRNA polymerase IIEfficient transcription initiationTranscription initiation complexSRB proteinsCTD proteinsExtragenic suppressorsCTD functionPolymerase IIPreinitiation complexTranscription initiationInitiation complexComplex bindsTruncation mutationsSRB2Srb5ProteinBiochemical evidenceComplexesSRB4