2023
DOT1L promotes spermatid differentiation by regulating expression of genes required for histone-to-protamine replacement
Malla A, Rainsford S, Smith Z, Lesch B. DOT1L promotes spermatid differentiation by regulating expression of genes required for histone-to-protamine replacement. Development 2023, 150 PMID: 37082969, PMCID: PMC10259660, DOI: 10.1242/dev.201497.Peer-Reviewed Original ResearchConceptsHistone replacementMale sterilityProtamine exchangeSpermatid differentiationHistone H3 lysine 79Chromatin remodeling factorsRNA polymerase IIH3 lysine 79Expression of genesMature sperm headSperm headPostmeiotic germ cellsHistone methyltransferase DOT1LPolymerase IILysine 79Embryonic lethalityRemodeling factorsProtamine transitionProtamine replacementTranscriptional dysregulationMethyltransferase DOT1LIndispensable regulatorDOT1LHistonesGerm cells
2020
H3K4me1 Distribution Predicts Transcription State and Poising at Promoters
Bae S, Lesch BJ. H3K4me1 Distribution Predicts Transcription State and Poising at Promoters. Frontiers In Cell And Developmental Biology 2020, 8: 289. PMID: 32432110, PMCID: PMC7214686, DOI: 10.3389/fcell.2020.00289.Peer-Reviewed Original ResearchGerm cellsGene regulatory statesDifferent epigenetic marksTranscription start siteEmbryonic stem cellsMouse germ cellsGene expression levelsTranscription stateChromatin stateEpigenetic memoryEpigenetic stateEpigenetic marksLysine 4Histone H3Somatic cellsDistal enhancerStart siteActive promotersH3K4me1Transcriptional activityPromoter regionH3K4me3Possible rolePromoterCell types
2016
Parallel evolution of male germline epigenetic poising and somatic development in animals
Lesch BJ, Silber SJ, McCarrey JR, Page DC. Parallel evolution of male germline epigenetic poising and somatic development in animals. Nature Genetics 2016, 48: 888-894. PMID: 27294618, DOI: 10.1038/ng.3591.Peer-Reviewed Original Research
2014
Poised chromatin in the mammalian germ line
Lesch BJ, Page DC. Poised chromatin in the mammalian germ line. Development 2014, 141: 3619-3626. PMID: 25249456, PMCID: PMC4197577, DOI: 10.1242/dev.113027.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMammalian germ lineGerm cell identityCell identityGerm lineMammalian germ cellsEmbryonic stem cellsChromatin stateEpigenetic inheritanceHistone modificationsRegulatory genesGene activationDNA methylationEpigenetic featuresChromatinGerm cellsStem cellsSomatic developmentGenesPromoterFetal stageTotipotencyCellsMeiosisGametogenesisRepression
2013
A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis
Lesch BJ, Dokshin GA, Young RA, McCarrey JR, Page DC. A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 16061-16066. PMID: 24043772, PMCID: PMC3791702, DOI: 10.1073/pnas.1315204110.Peer-Reviewed Original ResearchConceptsSet of genesGerm cellsPostmeiotic male germ cellsGerm line nucleiSexual differentiationRNA-seq analysisFemale germ cellsSubset of genesEmbryonic stem cellsMale germ cellsRegulators of differentiationEnd of meiosisCompletion of meiosisMouse germ cellsTotipotent zygoteDifferentiated gametesMulticellular organismsGamete precursorsDevelopmental genesChIP-seqGerm lineGamete unionExtraembryonic tissuesMeiotic initiationPostmeiotic cellsThe Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells
Okumura LM, Lesch BJ, Page DC. The Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells. PLOS ONE 2013, 8: e66062. PMID: 23762465, PMCID: PMC3676325, DOI: 10.1371/journal.pone.0066062.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDown-RegulationExonsFemaleFetusGene Expression Regulation, DevelopmentalGene TargetingGerm CellsHomeodomain ProteinsLigandsMeiosisMiceMice, Inbred C57BLMice, Mutant StrainsMutationNanog Homeobox ProteinNuclear Receptor Subfamily 6, Group A, Member 1Octamer Transcription Factor-3OogenesisOvaryPhenotypePluripotent Stem CellsProtein BindingProtein Structure, TertiarySequence DeletionStructure-Activity RelationshipConceptsFetal ovarian germ cellsOvarian germ cellsPluripotency genesSomatic cellsGerm cellsSilencing of Oct4Initiation of meiosisEmbryonic stem cellsLigand binding domainsGCNF geneEmbryonic day 14.5Tamoxifen-inducible CreDifferent developmental timepointsBinding domainsDifferentiated stateGCNFDevelopmental timepointsNanogGenesConditional ablationStem cellsDay 14.5CellsExpressionE8.0
2012
Genetics of germ cell development
Lesch BJ, Page DC. Genetics of germ cell development. Nature Reviews Genetics 2012, 13: 781-794. PMID: 23044825, DOI: 10.1038/nrg3294.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsGerm cell developmentGerm cellsHaploid genomeMultiple speciesCell developmentPluripotent stem cell systemsRepressive chromatin configurationGerm cell identityRNA polymerase IIGerm cell biologyStem cell systemEarly germ cellsPolymerase IICell identityDiploid genomeChromatin configurationIndividual organismsCell biologyMitotic proliferationGenomeSame speciesDifferent speciesGermline precursorsLater stepsMeiosis