Reversed-phase liquid chromatographic purification and isolation of a radio-iodinated selective probe for mu opioid receptors in the brain
Miller M, Gould B, Joshi D, Bennett H, James S, Billiar R. Reversed-phase liquid chromatographic purification and isolation of a radio-iodinated selective probe for mu opioid receptors in the brain. Journal Of Neuroscience Methods 1992, 41: 93-99. PMID: 1314320, DOI: 10.1016/0165-0270(92)90052-f.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoradiographyBrainChromatography, High Pressure LiquidD-Ala(2),MePhe(4),Met(0)-ol-enkephalinFemaleIodine RadioisotopesMiceMice, Inbred C57BLReceptors, OpioidReceptors, Opioid, muConceptsAdrenocorticotropin hormoneBlock ligand bindingOpioid receptorsMouse corpus striatumN-methyl-Phe4Nucleus accumbens septiGly-ol5 enkephalinDiagonal band of BrocaDecreased ligand bindingLight microscopic autoradiographyPresence of agonistAccumbens septiHigh-performance liquid chromatography columnLigand bindingD-Ala2Beta-endorphinCorpus striatumRadioligand bindingLiquid chromatography columnDiagonal bandCingulate cortexAnalysis of ligandsIncreasing dosesIodine reagentsLiquid chromatographic purification